15期

最新文章

• Proximity- activated DNA scanning encoded sequencing for massive access to membrane proteins nanoscale organization AI翻译
  作者：Zhao, Xueqi; Zhao, Yue; Li, Zhu; Liu, Huan; Fu, Wenhao; Chen, Feng; Sun, Ying; Song, Daqian; Fan, Chunhai; Weitz, David; Zhao, Yongxi
  作者单位：Jilin University; Xi'an Jiaotong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Xi'an Jiaotong University; Xi'an Jiaotong University
  摘要：Cellular structure maintenance and function regulation critically depend on the composition and spatial distribution of numerous membrane proteins. However, current methods face limitations in spatial coverage and data scalability, hindering the comprehensive analysis of protein interactions in complex cellular nanoenvironment. Herein, we introduce proximity-activated DNA scanning encoded sequencing (PADSE-seq), an innovative technique that utilizes flexible DNA probes with adjustable lengths....
• DprA recruits ComM to facilitate recombination during natural transformation in Gram- negative bacteria AI翻译
  作者：Dalia, Triana N.; Machouri, Merick; Lacrouts, Celine; Fauconnet, Yoann; Guerois, Raphael; Andreani, Jessica; Radicella, J. Pablo; Dalia, Ankur B.
  作者单位：Indiana University System; Indiana University Bloomington; Universite Paris Saclay; CEA; Universite Paris Cite; Universite Paris Cite; CEA; Universite Paris Saclay; Centre National de la Recherche Scientifique (CNRS); CEA
  摘要：Natural transformation (NT) represents one of the major modes of horizontal gene transfer in bacterial species. During NT, cells can take up free DNA from the environment and integrate it into their genome by homologous recombination. While NT has been studied for >90 y, the molecular details underlying this recombination remain poorly understood. Recent work has demonstrated that ComM is an NT-specific hexameric helicase that promotes recombinational branch migration in Gram-negative bacteria...
• Bourbon and Mycbp function with Otu to promote Sxl protein expression in the Drosophila female germline AI翻译
  作者：Mercer, Marianne; Dasgupta, Anirban; Pawlowski, Krzysztof; Buszczak, Michael
  作者单位：University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; Howard Hughes Medical Institute
  摘要：In Drosophila ovaries, germ cells differentiate through several stages of cyst development before entering meiosis. This early differentiation program depends on both the stepwise deployment of specific regulatory mechanisms and on maintenance of germline sexual identity. The study of female sterile mutations that result in formation of germ cell tumors has been invaluable in identifying the mechanisms that control these developmental events. Here, we characterize the germ cell-enriched gene b...
• Downregulation of Nesprin1 by Runx2 deficiency is critical for the development of skeletal laminopathy-like pathology AI翻译
  作者：Saito, Akiko; Nagayama, Kazuaki; Okada, Hiroyuki; Onodera, Shoko; Aida, Natsuko; Nakamura, Takashi; Sawada, Takashi; Hojo, Hironori; Kato, Shigeaki; Azuma, Toshifumi
  作者单位：Tokyo Dental College; Tokyo Dental College; Ibaraki University; University of Tokyo; University of Tokyo; Tokyo Dental College; Iryo Sosei University
  摘要：Runx2 is a master regulator of bone formation, and its dysfunction causes cleidocranial dysplasia (CCD) in humans. When iPS cells were generated from patients with CCD and Runx2-deficient iPS cells were generated using gene-editing techniques, abnormal laminopathy-like nuclei were observed. Runx2-deficient cells showed reduced Lamin A/C expression, but not protein levels. However, in Runx2-deficient cells, both the gene expression and protein levels of Nesprin1 were reduced, perinuclear actin ...
• Emergent gauge fields in band insulators AI翻译
  作者：Han, Zhaoyu; Kivelson, Steven A.
  作者单位：Stanford University
  摘要：By explicit microscopic construction involving a mapping to a quantum vertex model subject to the ice rule, we show that an electronically trivial band insulator with suitable vibrational (phonon) degrees of freedom can host a resonating valence-bond state-a quantum phase with emergent gauge fields. This type of band insulator is identifiable by the existence of emergent gapless photon modes and deconfined excitations, the latter of which carry nonquantized mobile charges. We suggest that such...
• Structural basis for immune cell binding of Fusobacterium nucleatum via the trimeric autotransporter adhesin CbpF AI翻译
  作者：Marongiu, Gian Luca; Fink, Uwe; Schoepf, Felix; Oder, Andreas; von Kries, Jens Peter; Roderer, Daniel
  摘要：Fusobacterium nucleatum (Fn), a commensal in the human oral cavity, is overrepresented in the colon microbiota of colorectal cancer (CRC) patients and is linked to tumor chemoresistance, metastasis, and a poor therapeutic prognosis. Fn produces numerous adhesins that mediate tumor colonization and downregulation of the host's antitumor immune response. One of these, the trimeric autotransCEACAM1 on T- cells and has been associated with immune evasion of Fn- colonized tumors. Whereas the role o...
• Enamel-dentine junction morphology reveals population replacement and mobility in the late prehistoric Middle Nile Valley AI翻译
  作者：Martin, Nicolas; Thibeault, Adrien; Varadzinova, Lenka; Usai, Donatella; Ambrose, Stanley H.; Antoine, Daniel; Havelkova, Petra Brukner; Honegger, Matthieu; Irish, Joel D.; Jesse, Friederike; Marechal, Laura; Osypinska, Marta; Osypinski, Piotr; Santos, Frederic; Vanderesse, Nicolas; Varadzin, Ladislav; Whiting, Rebecca J.; Zanolli, Clement; Veleminsky, Petr; Crevecoeur, Isabelle
  作者单位：Universite de Bordeaux; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Ecology & Environment (INEE); Charles University Prague; University of Illinois System; University of Illinois Urbana-Champaign; National Museum; Liverpool John Moores University; University of Wuppertal; University of Geneva; University of Wroclaw; Polish Academy of Sciences; Institute of Archaeology & Ethnology of the Polish Academy of Sciences; Czech Academy of Sciences; Institute of Archaeology of the Czech Academy of Sciences - Prague
  摘要：Transitions from foraging to food-production represent a worldwide turning point in recent human history. In the Middle Nile Valley this cultural shift occurred between the sixth and beginning of the fifth millennium BCE. Significant craniodental morphological differences remain inadequately tested by biometric analyses of ancestry and may reflect population origins or diet change between the last hunter-fisher-gatherers (Mesolithic) and first food-producers (Neolithic). Moreover, with no anci...
• Arf1 and ARFGEF2/Sec71 control neuroblast polarity by anchoring nonmuscle myosin II AI翻译
  作者：Gujar, Mahekta R.; Tan, Ye Sing; Gao, Yang; Wang, Hongyan
  作者单位：National University of Singapore; National University of Singapore; National University of Singapore
  摘要：Neural stem cells (NSCs) can self- renew and undergo differentiation via asymmetric division. Dysregulation in the balance between self- renewal and differentiation can lead to tumor formation or neurodevelopmental disorders. However, the regulation localization during asymmetric division in dividing cells is not well established. Here, we show that the Golgi proteins Arf1 and ARFGEF2/Sec71 control asymmetric division of Drosophila NSCs by facilitating the localization of myosin II regulatory ...
• FAO-fueled OXPHOS and NRF2mediated stress resilience in MICs drive lymph node metastasis AI翻译
  作者：Li, Shan-Shan; Zhang, Baifeng; Huang, Cuicui; Fu, Yuying; Zhao, Yuying; Gong, Lanqi; Tan, Yanan; Wang, Huali; Chen, Wenqi; Luo, Jie; Zhang, Yu; Ma, Stephanie; Fu, Li; Liu, Chenli; Huang, Jiandong; Ju, Huai-Qiang; Lee, Anne Wing-Mui; Guan, Xin-Yuan
  作者单位：University of Hong Kong; Advanced Energy Science & Technology Guangdong Laboratory; University of Hong Kong; State Key Lab Oncology South China; Sun Yat Sen University; University of Hong Kong; Shenzhen University; Chinese Academy of Sciences; Shenzhen Institute of Advanced Technology, CAS
  摘要：Metastasis is an inefficient process requiring cancer cells to adapt metabolically for survival and colonization in new environments. The contributions of tumor metabolic reprogramming to lymph node (LN) metastasis and its underlying mechanisms remain elusive. Through single-cell RNA sequencing, we identified rare metastasis-initiating cells (MICs) with stem-like properties that drive early LN metastasis. Integrated transcriptome, lipidomic, metabolomic, and functional analyses demonstrated th...
• Degenerate domain walls in supersymmetric theories AI翻译
  作者：Che, Shi; Ievlev, Evgenii; Shifman, Mikhail
  作者单位：University of Minnesota System; University of Minnesota Twin Cities; University of Minnesota System; University of Minnesota Twin Cities
  摘要：In supersymmetric Yang-Mills theories tension-degenerate domain walls are typical. Adding matter fields in fundamental representation, we arrive at supersymmetric quantum chromodynamics (SQCD) supporting similar walls. We demonstrate that the degenerate domain walls can belong to one of two classes: i) locally distinguishable, i.e. those which differ from each other locally (which could be detected in local measurements); and ii) those which have identical local structure and are differentiate...