Structural basis for immune cell binding of Fusobacterium nucleatum via the trimeric autotransporter adhesin CbpF

• 成果类型: Article
• 署名作者: Marongiu, Gian Luca; Fink, Uwe; Schoepf, Felix; Oder, Andreas; von Kries, Jens Peter; Roderer, Daniel
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8992
• DOI: 10.1073/pnas.2418155122
• 发表日期: 2025-04-15
• 关键词: carcinoembryonic antigen family; cryo-em; molecule; receptor; ceacam1; protein; uspa1; expression; cd66a

摘要

Fusobacterium nucleatum (Fn), a commensal in the human oral cavity, is overrepresented in the colon microbiota of colorectal cancer (CRC) patients and is linked to tumor chemoresistance, metastasis, and a poor therapeutic prognosis. Fn produces numerous adhesins that mediate tumor colonization and downregulation of the host's antitumor immune response. One of these, the trimeric autotransCEACAM1 on T- cells and has been associated with immune evasion of Fn- colonized tumors. Whereas the role of CEACAM1 in homophilic and heterophilic cell interactions and immune evasion is well described, the mechanistic details of its interaction with fusobacterial CbpF remain unknown due to the lack of a high- resolution structure of the adhesin-receptor complex. Here, we present two structures of CbpF alone and in complex with CEACAM1, obtained by cryogenic electron microscopy and single particle analysis. They reveal that CbpF forms a stable homotrimeric complex whose N- terminal part of the extracellular domain comprises a 64 & Aring; long beta roll domain with a unique lateral loop extension. CEACAM1 binds to this loop with high affinity via its N- terminal IgV- like domain with a nanomolar dissociation constant as determined by surface plasmon resonance. This study provides a comprehensive structural description of a fusobacterial TAA, illustrates a yet undescribed CEACAM1 binding mode, and paves the way for rational drug design targeting Fn in CRC.