Arf1 and ARFGEF2/Sec71 control neuroblast polarity by anchoring nonmuscle myosin II

• 成果类型: Article
• 署名作者: Gujar, Mahekta R.; Tan, Ye Sing; Gao, Yang; Wang, Hongyan
• 署名单位: National University of Singapore; National University of Singapore; National University of Singapore
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9215
• DOI: 10.1073/pnas.2419468122
• 发表日期: 2025-04-15
• 关键词: guanine-nucleotide-exchange; asymmetric cell-division; balancing self-renewal; light-chain; spindle orientation; neural progenitors; tumor suppressors; factors big1; stem-cells; g-proteins

摘要

Neural stem cells (NSCs) can self- renew and undergo differentiation via asymmetric division. Dysregulation in the balance between self- renewal and differentiation can lead to tumor formation or neurodevelopmental disorders. However, the regulation localization during asymmetric division in dividing cells is not well established. Here, we show that the Golgi proteins Arf1 and ARFGEF2/Sec71 control asymmetric division of Drosophila NSCs by facilitating the localization of myosin II regulatory light chain, Sqh, to the NSC cortex. Arf1 can physically associate with Sqh and Vibrator, a type I PITP that stimulates phospholipid PI4K activity for PI(4)P production. Further, Arf1 and Sec71 facilitate PI(4)P localization to the cell cortex of neuroblasts. Our data provide evidence that the Golgi proteins Arf1 and its GEF Sec71 facilitate neuroblast polarity through phospholipid- dependent nonmuscle myosin II cortical localization.