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作者：Bragado, Laureano; Pozzi, Berta; Beckerman, Ines; Magalnik, Melina; Srebrow, Anabella
作者单位：University of Buenos Aires; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); University of Buenos Aires
摘要：The noncoding small nuclear RNAs (snRNAs) associate with a large set of proteins to form small nuclear ribonucleoprotein particles (snRNPs). While the function of snRNAs is well characterized, the regulation of their transcription remains poorly understood. Recently, we demonstrated that SUMO conjugation regulates snRNA3 ' end processing. To further study the connection between SUMOylation and snRNA biogenesis, we generated a CRISPR/dCas9 tool comprising a catalytically inactive Cas9 (dCas9) f...
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作者：Chuang, Po-Kai; Chung, Cinya; Liao, Kuo-Shiang; Wong, Chi-Huey
作者单位：Scripps Research Institute; National Sun Yat Sen University; Kaohsiung Medical University; Kaohsiung Medical University Hospital; Academia Sinica - Taiwan
摘要：Pancreatic cancer remains one of the most lethal malignancies with limited therapeutic options. Among the alternative targets explored for pancreatic cancer, the glycolipid stage-specific embryonic antigen-4 (SSEA4) has been extensively studied. We have previously demonstrated that the chimeric anti-SSEA4 antibody with alpha-2,6-sialylated complex type biantennary glycan (SCT) or its 3-fluorosialyl derivative (FSCT) attached to the Fc-Asn297 residue exhibited enhanced binding to the Fc-recepto...
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作者：Chen, Zhengyun; Lian, Justin Z.; Huang, Haozhou; Zhu, Hengyi; Kanye, Lynda Thubelihle; Chen, Mateng; Zhang, Zhihao; Liu, Boxin; Zhang, Jiawei; Ahmed, Ajaz; Barbarossa, Valerio; Cucurachi, Stefano; Dong, Bin
作者单位：China Pharmaceutical University; Leiden University; Leiden University - Excl LUMC; New York University; China Pharmaceutical University; China Pharmaceutical University; Imperial College London; University of California System; University of California Los Angeles; China Pharmaceutical University
摘要：This study presents a comparative cradle-to-gate environmental and economic evaluation of nine synthetic routes for Molnupiravir (EIDD-2801), a broad-spectrum antiviral drug. Integrating Life Cycle Assessment (LCA) with Life Cycle Costing (LCC), we quantified the environmental impacts alongside capital and operational costs, net present costs, and break-even prices of each route. The results reveal significant variation across the schemes, with Schemes #1 and #2 exhibiting the highest impacts ...
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作者：Angelini, Maria Chiara; Palazzi, Saverio; Parisi, Giorgio; Rizzo, Tommaso
作者单位：Sapienza University Rome; Istituto Nazionale di Fisica Nucleare (INFN); Beihang University; Consiglio Nazionale delle Ricerche (CNR); Istituto di Nanotecnologia (NANOTEC-CNR); Consiglio Nazionale delle Ricerche (CNR); Istituto dei Sistemi Complessi (ISC-CNR)
摘要：We analyze the spin-glass transition in a field in finite dimension D below the upper critical dimension directly at zero temperature using a recently introduced perturbative loop expansion around the Bethe lattice solution. The expansion is generated by the so-called M-layer construction, and it has 1/M as the associated small parameter. Computing analytically and numerically these nonstandard diagrams at first order in the 1/M expansion, we construct an e-expansion around the upper critical ...
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作者：Jiang, Zhimin; Pan, Wenliang; Chen, Yu; Zhou, Dongliang; Ren, Shuning; Tong, Qi; Liu, Litao; Sun, Honglei; Sun, Yipeng; Bi, Yuhai; Wang, Dayan; Lu, Lu; Pu, Juan; Chang, Kin - Chow; Liu, Jinhua
作者单位：China Agricultural University; Chinese Academy of Sciences; Institute of Microbiology, CAS; Chinese Center for Disease Control & Prevention; National Institute for Viral Disease Control & Prevention, Chinese Center for Disease Control & Prevention; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); Roslin Institute; University of Edinburgh; University of Nottingham
摘要：Influenza A virus (IAV) infection causes significantly greater morbidity and mortality in the elderly population, but the molecular mechanisms in the aging process responsible for severe infection remain unclear. In this study, we found that increased severity in IAV infection and reduced innate immune response correlated with extensive mitophagy in senescent human cells and in the lung of aged mice. Apolipoprotein D (ApoD) was identified as strongly elevated in the lungs and sera of aged huma...
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作者：Yu, Hongsheng; Pathak, Amit
作者单位：Washington University (WUSTL)
摘要：During wound healing, tumor growth, and organ formation, epithelial cells migrate and cluster in layered tissue environments. Although cellular mechanosensing of adhered extracellular matrices is now well recognized, it is unclear how deeply cells sense through distant matrix layers. Since single cells can mechanosense stiff basal surfaces through soft hydrogels of <10 mu m thickness, here we ask whether cellular collectives can perform such depth-mechanosensing through thicker matrix layers. ...
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作者：Afzal, Junaid; Suhail, Yasir; Du, Wenqiang; Liu, Yamin; Ramasamy, Ramalakshmi; Liu, Zukai; Goyal, Ruchi; Novin, Ashkan; Suhail, Sameera; Maziarz, Jamie; Wali, Khadija; Robson, Paul; Wagner, Gunter P.; Kshitiz
作者单位：University of California System; University of California San Francisco; University of Connecticut; Jackson Laboratory; Yale University; Yale University
摘要：Coopetition is a term from game theory that describes a mix of cooperative and competitive behavior. The maternal-fetal interface (MFI) among eutherian mammals presents close interaction of two distinct individuals. These interactions have resulted in a remarkable diversity in MFI structure, often interpreted as the outcome of maternal-fetal conflict. Nevertheless, the fetus and the mother share evolutionary interests since 50% of fetal genes are maternal. In hemochorial species, characterized...
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作者：Ando, Jun; Murai, Kazue; Michiyuki, Tomoe; Takahashi, Ikuko; Iida, Tatsuya; Kogo, Yasushi; Toyoda, Masashi; Saito, Yuko; Murayama, Shigeo; Kurihara, Masanori; Watanabe, Rikiya
作者单位：RIKEN
摘要：Digital bioanalysis enables highly sensitive detection of biomolecules at the single-molecule level, making it a widely used technique in biomedical research. However, conventional approaches typically rely on fluorescence detection of single-enzyme reactions, which limits molecular selectivity and the ability to analyze multiple targets simultaneously. To address these limitations, we developed a digital bioanalysis platform based on surface-enhanced Raman scattering spectroscopy and microcha...
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作者：Mittal, Anshumali; Martin, Matthew F.; Provins, Laurent; Hall, Adrian; Ledecq, Marie; Wolff, Christian; Gillard, Michel; Horanyi, Peter S.; Coleman, Jonathan A.
作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; UCB Pharma SA
摘要：Brivaracetam (BRV) and levetiracetam (LEV) are antiseizure medications (ASMs); UCB-J is a PET tracer targeting synaptic vesicle protein 2A (SV2A); UCB7361 is closely related to padsevonil, an experimental anticonvulsant; while UCB1244283 acts as an allosteric modulator for BRV and LEV binding but not for these other ligands. The SV2A-BRV-UCB1244283 structure reveals how UCB1244283 allosterically enhances BRV binding by occupying an allosteric site near the primary binding site, preventing BRV ...
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作者：Regev, Johanna; Szanton, Nahshon; Vukosavovic, Filip; Berko, Itamar; Shalev, Yiftah; Uziel, Joe; Mintz, Eugenia; Regev, Lior; Boaretto, Elisabetta
作者单位：Weizmann Institute of Science
摘要：Using well-established microarchaeological sampling methods, we reached a precise radiocarbon date of 800 BC for the Siloam Pool's monumental water dam in Jerusalem. This date is a critical link connecting several imposing waterworks constructed at that time. Climate data pointing to droughts and flash floods during the last decades of the 9th century BC provide a logical framework for the reasons behind such endeavors. These included the fortification of the city's primary water source, the G...
