2025

最新文章

• TIGER: A tdTomato in vivo genome- editing reporter mouse for investigating precision- editor delivery approaches AI翻译
  作者：Du, Samuel W.; Palczewska, Grazyna; Dong, Zhiqian; Lauterborn, Julie C.; Kaipa, Balasankara Reddy; Yan, Alexander L.; Holubowicz, Rafa; Ha, Siyoung; Chen, Paul Z.; Gall, Christine M.; Zode, Gulab; Liu, David R.; Palczewski, Krzysztof
  作者单位：University of California System; University of California Irvine; University of California System; University of California Irvine; University of California System; University of California Irvine; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Howard Hughes Medical Institute; Harvard University; Massachusetts Institute of Technology (MIT); University of California System; University of California Irvine; University of California System; University of California Irvine
  摘要：In vivo genome editing has the potential to address many inherited and environmental disorders. However, a major hurdle for the clinical translation of genome editing is safe, efficient delivery to disease-relevant tissues. A modality-agnostic reporter animal model that facilitates rapid, precise, and quantifiable assessment of functional delivery and editing could greatly enhance the evaluation and translation of delivery technologies. Here, we present the development of the tdTomato in vivo ...
• Solstice, selection, and synchrony of seed masting AI翻译
  作者：Bogdziewicz, Michal; Hacket-Pain, Andrew; Kelly, Dave; Szymkowiak, Jakub; Foest, Jessie; Journe, Valentin
  作者单位：Adam Mickiewicz University; University of Liverpool; University of Canterbury; Adam Mickiewicz University; Kyushu University
• Object-directed action representations are componentially built in parietal cortex AI翻译
  作者：Caglar, Leyla Roksan; Walbrin, Jon; Akwayena, Emefa; Almeida, Jorge; Mahon, Bradford Z.
  作者单位：Carnegie Mellon University; Universidade de Coimbra; Universidade de Coimbra; Carnegie Mellon University; University of Rochester
  摘要：The inferior parietal lobule supports action representations that are necessary to grasp and 71-78 (2004)]. The supramarginal gyrus (SMG) is a structure within the inferior parietal lobule that specifically processes object-directed patterns of manipulation during functional object use. Here, we demonstrate that neural representations of complex object-directed actions in the SMG can be predicted by a linear encoding model that componentially builds complex actions from an empirically defined ...
• Synaptic transmission is dispensable for selecting the winner input but is crucial for the subsequent events of synapse elimination AI翻译
  作者：Kao, Tzu- Huei; Okuno, Yuto; Matsuyama, Kyoko; Watanabe, Takaki; Uesaka, Naofumi; Kano, Masanobu
  作者单位：University of Tokyo; University of Tokyo; Teikyo University; Institute of Science Tokyo
  摘要：Synaptic transmission has long been thought to regulate neuronal wiring during postnatal development, but this assumption remains largely untested. Selective strengthening of a single winner climbing fiber (CF) afferent to each Purkinje cell (PC) and elimination of the other loser CF axons in the cerebellum has been a representative model of neural circuit refinement. Here, we examined the role of neurotransmission at CF-PC synapses in their postnatal development. We labeled a subset of CFs in...
• RyR1-mediated Ca2+- induced Ca2+release plays a negligible role in excitation-contraction coupling of normal skeletal muscle AI翻译
  作者：Kobayashi, Takuya; Yamazawa, Toshiko; Kurebayashi, Nagomi; Konishi, Masato; Tanihata, Jun; Sugihara, Masami; Miki, Yoshifumi; Noguchi, Satoru; Inoue, Yukiko U.; Inoue, Takayoshi; Sakurai, Takashi; Murayama, Takashi
  作者单位：Jikei University; Juntendo University; Jikei University; Jikei University; Jikei University; Juntendo University; National Center for Neurology & Psychiatry - Japan; National Center for Neurology & Psychiatry - Japan
  摘要：Type 1 ryanodine receptor (RyR1) is a Ca2+ release channel in the sarcoplasmic reticulum in skeletal muscle. In excitation-contraction (E-C) coupling, RyR1 opens by depolarization of transverse tubule membrane via physical interaction with dihydropyridine receptor, which is referred to as depolarization-induced Ca2+ release (DICR). RyR1 can also be gated via Ca2+-induced Ca2+ release (CICR), in which binding of Ca2+ directly opens the channel. Thus, RyR1 has two Ca2+ release modes; DICR and CI...
• Identification of broadly inhibitory anti-PfEMP1 antibodies by mass spectrometry sequencing of plasma IgG from a malaria- exposed child AI翻译
  作者：Turner, Louise; Diaz, Teresa Nunez de Villavicencio; Raghavan, Sai Sundar Rajan; Kana, Ikhlaq Hussain; Lyimo, Eric; Reitzel, Chelsea; Wang, Christian W.; Berube, Ewen; Jensen, Rasmus W.; Loeffler, Johannes R.; Fernandez, Monica Lisa; Theander, Thor G.; Lusingu, John P. A.; Le Bihan, Thierry; Han, Xiaobing; Minja, Daniel T. R.; Ward, Andrew B.; Ma, Bin; Lavstsen, Thomas
  作者单位：University of Copenhagen; University of Copenhagen; Copenhagen University Hospital; Rigshospitalet; Scripps Research Institute
  摘要：Plasmodium falciparum pathology is driven by the accumulation of parasite- infected erythrocytes in blood capillaries. This sequestration process is mediated by the parasite's P. falciparum erythrocyte membrane protein 1 (PfEMP1) adhesins, which bind select endothelial cell receptors. A subset of PfEMP1 binding human endothelial protein C receptor (EPCR) through their cysteine- rich interdomain region alpha 1 (CIDR alpha 1) domains drives the pathogenesis to severe malaria. Despite high sequen...
• A 65-kb deletion survey identifies a distal cis-regulatory region for red-light induction of Ghd7, a key rice floral repressor AI翻译
  作者：Ogo, Yuko; Kawauchi, Takumi; Mimura, Manaki; Naito, Ken; Itoh, Hironori; Izawa, Takeshi
  作者单位：National Agriculture & Food Research Organization - Japan; University of Tokyo; National Agriculture & Food Research Organization - Japan; National Agriculture & Food Research Organization - Japan
  摘要：The Ghd7 (Grain number, plant height, and heading date 7)gene integrates red light signals and circadian rhythms to control floral repression under long-day conditions in rice. CRISPR/Cas9 systems were employed to create a series of deletion mutant lines in the upstream regions of Ghd7, covering a 65-kb genomic region from its transcription start site (TSS). These deletions ranged from 2 to 25 kb in size. Three deletion lines, those from 0 to-3 kb (0/-3 K), -20 to-40 kb (-20/-40 K), and-26 to-...
• Eliminating hunger in a world serious about climate mitigation AI翻译
  作者：Burney, Jennifer A.
  作者单位：Stanford University; University of California System; University of California San Diego; University of California System; University of California San Diego; Scripps Institution of Oceanography
  摘要：What will it take to eliminate hunger in a world that is increasingly serious about climate change mitigation? For the past two decades, climate activism and mitigation efforts have largely focused on energy, industry, and transportation as the main contributors to anthropogenic warming. Although there is a long way to go to eliminate emissions from these sources, increasing international ambition means that attention is now turning to other hard-to-decarbonize sectors. Net zero food systems i...
• How a toxic pollutant avoids photodegradation: Relaxation pathways of UV-excited 2,4-dinitrophenol in aqueous solution AI翻译
  作者：Greene, Hallam J. M.; Ghosh, Deborin; Sazanovich, Igor V.; Phelps, Ryan; Mackintosh, Bembe C.; Curchod, Basile F. E.; Orr-Ewing, Andrew J.
  作者单位：University of Bristol; UK Research & Innovation (UKRI); Science & Technology Facilities Council (STFC); STFC Rutherford Appleton Laboratory
  摘要：Nitroaromatic compounds are pollutants emitted from biomass burning and fossil fuel combustion. They are a major component of brown carbon aerosols, affecting radiative forcing in the lower atmosphere. Among these species, 2,4-dinitrophenol (DNP) is toxic to both plants and animals and is resistant to photodegradation when dissolved in water, such as in aqueous atmospheric aerosols. To understand this environmental photostability of DNP, the photochemistry of near-UV excited DNP in aqueous sol...
• The activity and expression of adenylosuccinate lyase were reduced during modern human evolution, affecting brain and behavior AI翻译
  作者：Ju, Xiang - Chun; Lee, Shin - Yu; Agren, Richard; Machado, Luiz Carlos; Xing, Jiawei; Azama, Chika; Roy, Michael C.; Endo, Toshihiro; Huttner, Wieland; Siepel, Adam; Fukunaga, Izumi; Zeberg, Hugo; Paabo, Svante
  作者单位：Okinawa Institute of Science & Technology Graduate University; Karolinska Institutet; Max Planck Society; Max Planck Society
  摘要：Adenylosuccinate lyase (ADSL), an enzyme that is crucial for purine biosynthesis, carries an amino acid substitution that is present in almost all humans today but absent in Neandertals and Denisovans. This substitution reduces the stability of the enzyme, but what functional consequences it has are unknown. Here, we show that when introduced into mice, this substitution causes substrates of the enzyme to accumulate in amounts that correlate negatively with ADSL expression levels. In the brain...