-
作者：Darin, Emily; Farrell, Morgan v; Ali, Tatyana N.; Alfaro, Josefa Rivera; Malter, Kyle E.; Shikuma, Nicholas J.
作者单位：California State University System; San Diego State University; California State University System; San Diego State University
摘要：Diverse animals across the tree of life undergo the life- history transition of metamorphosis in response to bacteria. Although immunity has been implicated in this metamorphosis in response to bacteria, no functional connection has yet been demonstrated between immunity and metamorphosis. We investigated a host-microbe interaction involving Pseudoalteromonas luteoviolacea, a metamorphosis- inducing marine bacterium. By creating a marine bacteria-mediated RNA interference approach, we show tha...
-
作者：Kragelj, Jaka; Ghosh, Rupam; Xiao, Yiling; Dumarieh, Rania; Lagasca, Dominique; Krishna, Sakshi; Frederick, Kendra K.
作者单位：University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; National Institute of Chemistry - Slovenia
摘要：The protein alpha- syn adopts a wide variety of conformations including an intrinsically disordered monomeric form and an alpha- helical- rich membrane- associated form that is thought to play an important role in cellular membrane processes. However, despite the high affinity of alpha- syn for membranes, evidence that the alpha- helical form is adopted inside cells has been indirect. DNP- assisted solid- state NMR on frozen cellular samples can report directly on the entire conformational ens...
-
作者：Pappas, Kyrie; Ferrari, Matteo; Smith, Perianne; Nandakumar, Subhiksha; Khan, Zahra; Young, Serina B.; Laclair, Justin; Russo, Marco Vincenzo; Hobbs, Emmet Huang; Schultz, Nikolaus; Abida, Wassim; Karthaus, Wouter; Jasin, Maria; Sawyers, Charles L.
作者单位：Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; University of Minnesota System; University of Minnesota Twin Cities; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Columbia University; NewYork-Presbyterian Hospital; Albany Medical College; University of Miami; Memorial Sloan Kettering Cancer Center; Swiss Institute Experimental Cancer Research; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; Howard Hughes Medical Institute
摘要：Recent approvals of polymeric adenosine diphosphate ribose (poly(ADP-ribose) polymerase inhibitors (PARPi) for BRCA-mutant metastatic castration resistant prostate cancer necessitate an understanding of the factors that shape sensitivity and resistance. Reversion mutations that restore homologous recombination (HR) repair are detected in similar to 50 to 80% of BRCA-mutant patients who respond but subsequently relapse, but there is currently little insight into why only similar to 50% of BRCA-...
-
作者：Hui, Chenggong; de Vries, Reinier; Kopec, Wojciech; de Groot, Bert L.
作者单位：University of London; Queen Mary University London
摘要：Potassium (K+) channels are widely distributed in many types of organisms. They combine high efficiency (similar to 100 pS) and K+/Na+ selectivity by a conserved selectivity filter (SF). Molecular Dynamics (MD) simulations can provide detailed, atomistic mechanisms of this sophisticated ion permeation. However, currently there are clear inconsistencies between computational predictions and experimental results. First, the ion occupancy of the SF in simulations is lower than expected (similar t...
-
作者：Lobell, David B.; Di Tommaso, Stefania
作者单位：Stanford University; Stanford University
摘要：Efforts to anticipate and adapt to future climate can benefit from historical experiences. We examine agroclimatic conditions over the past 50 y for five major crops around the world. Most regions experienced rapid warming relative to interannual variability, with 45% of summer and 32% of winter crop area warming by more than two SD (6). Vapor pressure deficit (VPD), a key driver of plant water stress, also increased in most temperate regions but not in the tropics. Precipitation trends, while...
-
作者：Mandala, Venkata Shiva; MacKinnon, Roderick
作者单位：Rockefeller University; Howard Hughes Medical Institute
摘要：Gating in voltage-dependent ion channels is regulated by the transmembrane voltage. This form of regulation is enabled by voltage-sensing domains (VSDs) that respond to transmembrane voltage differences by changing their conformation and exerting force on the pore to open or close it. Here, we use cryogenic electron microscopy to study the neuronal K(v)2.1 channel in lipid vesicles with and without a voltage difference across the membrane. Hyperpolarizing voltage differences displace the posit...
-
作者：Zhao, Pengjun; Wang, Hao; Clark, William A. V.; Feng, Yongheng; Liu, Qiyang; Cui, Yanzhe
作者单位：Peking University; Peking University; Peking University Shenzhen Graduate School (PKU Shenzhen); Ministry of Natural Resources of the People's Republic of China; University of California System; University of California Los Angeles
摘要：The adoption of information and communication technology (ICT) by rural-urban migrants is reshaping job-search mobility, significantly shaping city-level workforce geographical diversity. This study provides compelling evidence of ICT's impact by examining China's cities. We introduce the rural-urban migrant workforce Geographical Diversity Index (GDI), a metric that captures the mobility patterns of 20 million migrant workers across Chinese cities from Q1 2019 to Q4 2023. This study highlight...
-
作者：Peter, Sandra; Riemer, Kai; West, Jevin D.
作者单位：University of Sydney; University of Washington; University of Washington Seattle
摘要：A growing body of research suggests that the recent generation of large language model (LLMs) excel, and in many cases outpace humans, at writing persuasively and empathetically, at inferring user traits from text, and at mimicking human-like conversation believably and effectively-without possessing any true empathy or social understanding. We refer to these systems as anthropomorphic conversational agents to aptly conceptualize the ability of LLM-based systems to mimic human communication so...
-
作者：Cai, Mengli; Ying, Jinfa; Lopez, Juan M.; Huang, Ying; Clore, G. Marius
作者单位：National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK); Pontificia Universidad Catolica del Peru; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
摘要：The bacterial transcriptional regulator RfaH comprises structurally and functionally distinct N- (NTD) and C- (CTD) terminal domains. The latter switches from a helical hairpin packed against the NTD to a five-stranded beta-roll upon displacement by RNA polymerase binding. Here, we use exchange-based NMR to probe fold-switching intermediates sampled by the isolated CTD. In addition to the predominant (similar to 76 to 77%), semistable beta-roll conformation (state A), we identify four structur...
-
作者：Butler, George; Baker, Joanna; Amend, Sarah R.; Pienta, Kenneth J.; Venditti, Chris
作者单位：University of London; University College London; Johns Hopkins University; Johns Hopkins Medicine; University of Reading
