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作者：Francis, Robert A.
作者单位：University of London; King's College London
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作者：Luo, Man; Yu, Xiaochun
作者单位：Fudan University; Westlake Laboratory; Westlake University; Westlake University
摘要：Mutations in the NBS1 gene result in Nijmegen breakage syndrome (NBS), and the gene encodes NBS1 that forms a complex with MRE11 and RAD50 and participates in DNA damage repair. However, the molecular mechanism by which NBS1 mutations cause clinical phenotypes of NBS, such as craniofacial dysmorphism, is still unclear. Here, we show that NBS1 localizes at the ribosomal DNA (rDNA) loci in nucleoli and interacts with ribosomal RNA (rRNA) transcription machinery including RNA polymerase I (PolI) ...
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作者：Morrison, Maike L.; Xue, Katherine S.; Rosenberg, Noah A.; Clark, Andrew
作者单位：Stanford University
摘要：Microbial communities vary across space, time, and individual hosts, generating a need for statistical methods capable of quantifying variability across multiple microbiome samples at once. To understand heterogeneity across microbiome samples from different host individuals, sampling times, spatial locations, or experimental replicates, we present FAVA (FST-based Assessment of Variability across vectors of relative Abundances), a framework for characterizing compositional variability across t...
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作者：Du, Liming; Freitas, Maria Angelica; Zhang, Jingzhu; Zhang, Jingzhu; Xue, Yuanyuan; Veettil, Reshma T.; Zhao, Zhiyu; Morrison, Sean J.
作者单位：University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; Howard Hughes Medical Institute; University of Texas System; University of Texas Southwestern Medical Center
摘要：In early postnatal and young adult bone marrow, Leptin receptor-expressing (LepR+) stromal cells and endothelial cells synthesize factors required for hematopoietic stem cell (HSC) maintenance, including Stem Cell Factor (SCF) and Cxcl12. However, little is known about how these stromal cells change during aging. We performed single- cell RNA sequencing of mouse bone marrow stromal cells at 2, 12, and 24 mo of age. We identified five transcriptionally distinct subsets of LepR+ cells, all of wh...
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作者：Breugem, Tim I.; Riesebosch, Samra; Zhang, Jingshu; Mykytyn, Anna Z.; Krabbendam, Lisette; Groen, Nathalie; Varela, Sivana Baptista; Schipper, Debby; Doel, Petra B. van den; van Acker, Romy; Stadhouders, Ralph; Lamers, Mart M.; Haagmans, Bart L.
作者单位：Erasmus University Rotterdam; Erasmus MC; Erasmus University Rotterdam; Erasmus MC; National University of Singapore; University of Amsterdam
摘要：Transmissibility of respiratory viruses is a complex viral trait that is intricately linked to tropism. Several highly transmissible viruses, including severe acute respiratory syndrome coronavirus 2 and Influenza viruses, specifically target multiciliated cells in the upper respiratory tract to facilitate efficient human- to- human transmission. In contrast, the zoonotic Middle East respiratory syndrome coronavirus (MERS- CoV) generally transmits poorly between humans, which is largely attrib...
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作者：Jakosky, Bruce M.
作者单位：University of Colorado System; University of Colorado Boulder
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作者：Skiba, Meredith A.; Canavan, Clare; Nemeth, Genevieve R.; Liu, Jinghan; Kanso, Ali; Kruse, Andrew C.
作者单位：Harvard University; Harvard Medical School; Northwestern University; Leipzig University
摘要：Antibodies have the potential to target G protein-coupled receptors (GPCRs) with high receptor, cellular, and tissue selectivity; however, few antibody ligands for GPCRs exist. Here, we describe a generalizable selection method to enrich for GPCR ligands from a synthetic camelid antibody fragment (nanobody) library. Our strategy yielded multiple nanobody ligands for the angiotensin II type I receptor (AT1R), a prototypical GPCR and important drug target. We found that nanobodies readily act as...
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作者：Papale, Andrea; Segueni, Julie; Elmaroufi, Hanae; Noordermeer, Daan; Holcman, David
作者单位：Universite PSL; Ecole Normale Superieure (ENS); CEA; Universite Paris Saclay; Centre National de la Recherche Scientifique (CNRS); University of Cambridge
摘要：Topologically associating domains (TADs) are sub-Megabase regions in vertebrate genomes with enriched intradomain interactions that restrict enhancer-promoter contacts across their boundaries. However, the mechanisms that separate TADs remain incompletely understood. Most boundaries between TADs contain CTCF binding sites (CBSs), which individually contribute to the blocking of Cohesin-mediated loop extrusion. Using genome-wide classification, here we show that the width of TAD boundaries form...
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作者：Zajic, Emma L. Moore; Zhao, Ruonan; McKinney, Mary C.; Yi, Kexi; Wood, Christopher; Trainor, Paul A.
作者单位：Stowers Institute for Medical Research; University of Kansas; University of Kansas Medical Center
摘要：Neural crest cells (NCC) comprise a heterogeneous population of cells with variable potency that contribute to nearly every tissue and organ throughout the body. Considered unique to vertebrates, NCC are transiently generated within the dorsolateral region of the neural plate or neural tube during neurulation. Their delamination and migration are crucial for embryo development as NCC differentiation is influenced by their final resting locations. Previous work in avian and aquatic species reve...
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作者：Vantomme, Gil; Devienne, Gabrielle; Hull, Jacob M.; Huguenard, John R.
作者单位：Stanford University
摘要：The medial prefrontal cortex (mPFC) and hippocampus are critical for memory retrieval, decision making, and emotional regulation. While ventral CA1 (vCA1) shows direct and reciprocal connections with mPFC, dorsal CA1 (dCA1) forms indirect pathways to mPFC, notably via the thalamic reuniens nucleus (Re). Neuroanatomical tracing has documented structural connectivity of this indirect pathway through Re however, its functional operation is largely unexplored. Here, we used in vivo and in vitro el...
