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作者：Kim, Do Yeon; Lee, So Yeon; Ha, Hyun Ji; Park, Hyun Ho
作者单位：Chung Ang University
摘要：The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strateg...
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作者：Hagen, Johannes; Laun, Lisa; Lucke, Charlotte; Palme, Marten
作者单位：Jonkoping University; Stockholm University
摘要：This study examines the long-term association between income and life expectancy in Sweden between 1960 and 2021. The study is based on register data that include all Swedish permanent residents aged 40 y and older. The results show that the gap in life expectancy between the top and bottom income percentiles widened substantially: For men, it increased from 3.5 yin the 1960s to 10.9 y by the 2010s, and for women, from 3.8 yin the 1970s to 8.6 y by the 2010s. Despite a reduction in income ineq...
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作者：He, Jing; Burova, Elena; Taduriyasas, Chandrika; Ni, Min; Adler, Christina; Wei, Yi; Negron, Nicole; Xiong, Kun; Bai, Yu; Shavlakadze, Tea; Ioffe, Ella; Lin, John C.; Ferrando, Adolfo; Glass, David J.
作者单位：Regeneron
摘要：Splenic T cells are pivotal to the immune system, yet their function deteriorates with age. To elucidate the specific aspects of T cell biology affected by aging, we conducted a comprehensive multi-time point single-cell RNA sequencing study, complemented by single-cell Assay for Transposase Accessible Chromatin (ATAC) sequencing and single-cell T cell repertoire (TCR) sequencing on splenic T cells from mice across 10 different age groups. This map of age-related changes in the distribution of...
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作者：Kirschbaum, Carla; Bennett, Jack L.; Tian, Qiaoyu; Sen, Navoneel; Smith, Iain P. S.; Wu, Di; Benesch, Justin L. P.; Khalid, Syma; Isom, Georgia; Robinson, Carol, V
作者单位：University of Oxford; University of Oxford; University of Oxford; University of Oxford
摘要：The maintenance of lipid asymmetry (Mla) system in gram- negative bacteria transfers phospholipids between the outer and inner membrane to maintain the outer membrane asymmetry. Misplaced phospholipids are extracted from the outer leaflet of the outer membrane by MlaA, transferred to the periplasmic lipid transporter MlaC, and shuttled to the inner membrane. We set out to investigate the lipid transfer between MlaA and MlaC using native mass spectrometry, with the aim of determining the lipid ...
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作者：Lam, Wai Hei; Yu, Daqi; Zhang, Qiongdan; Lin, Yuhan; Li, Ningning; Li, Jian; Wu, Yue; Zhang, Yingyi; Gao, Ning; Tye, Bik Kwoon; Zhai, Yuanliang; Dang, Shangyu
作者单位：Hong Kong University of Science & Technology; University of Hong Kong; Peking University; Cornell University
摘要：In eukaryotes, the origin recognition complex (ORC) promotes the assembly of minichromosome maintenance 2 to 7 complexes into a head-to-head double hexamer at origin DNA in a process known as replication licensing. In this study, we present a series of cryoelectron microscopy structures of yeast ORC mutants in complex with origin DNA. We show that Orc6, the smallest subunit of ORC, utilizes its transcription factor II B-B domain to orchestrate the sequential binding of ORC to origin DNA. In ad...
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作者：Clement, Francois; Doerr, Carola; Klamroth, Kathrin; Paquete, Luis
作者单位：University of Washington; University of Washington Seattle; Centre National de la Recherche Scientifique (CNRS); Sorbonne Universite; University of Wuppertal; Universidade de Coimbra
摘要：Uniformly distributed point sets of low discrepancy are heavily used in experimental design and across a very wide range of applications such as numerical integration, computer graphics, and finance. Recent methods based on Graph Neural Networks [T. K. Rusch, N. Kirk, M. M. Bronstein, C. Lemieux, D. Rus, Proc. Natl. Acad. Sci. U.S.A. 121, e2409913121 (2024).] and solver-based optimization identified point sets having much lower discrepancy than previously known constructions. We show in this n...
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作者：Jenewein, Christian; Maiz-Sicilia, Aurora; Rull, Fernando; Gonzalez-Souto, Lorena; Garcia-Ruiz, Juan Manuel
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作者：Jones, Christopher P.; Ferre-D'Amare, Adrian R.
作者单位：National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
摘要：A hallmark of retrovirus replication is the translation of two different polyproteins from one RNA through programmed-1 frameshifting. This is a mechanism in which the actively translating ribosome is induced to slip in the 5 ' direction at a defined codon and then continues translating in the new reading frame. Programmed frameshifting controls the stoichiometry of viral proteins and is therefore under stringent evolutionary selection. Forty years ago, the first frameshifting stimulatory elem...
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作者：Adam, David
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作者：Colameo, David; Maley, Sara M.; Winterer, Jochen; ElGrawani, Waleed; Gilardi, Carlotta; Galkin, Simon; Fiore, Roberto; Brown, Steven A.; Schratt, Gerhard
作者单位：Swiss Federal Institutes of Technology Domain; ETH Zurich; University of Zurich
摘要：Homeostatic synaptic plasticity (HSP) is a neuronal mechanism that allows networks to compensate for prolonged changes in activity by adjusting synaptic strength. This process is crucial for maintaining stable brain function and has been implicated in memory consolidation during sleep. While scaling of both excitatory and inhibitory synapses plays an important role during homeostatic synaptic plasticity, molecules coordinating these processes are unknown. In this study, we investigate the role...
