AcrIE7 inhibits the CRISPR- Cas system by directly binding to the R- loop single- stranded DNA

• 成果类型: Article
• 署名作者: Kim, Do Yeon; Lee, So Yeon; Ha, Hyun Ji; Park, Hyun Ho
• 署名单位: Chung Ang University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11748
• DOI: 10.1073/pnas.2423205122
• 发表日期: 2025-04-08
• 关键词: anti-crispr; mechanisms

摘要

The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strategy distinct from previously reported Acr mechanisms and offers insights into CRISPR-Cas inhibition.