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作者：Brandt, Anthony; Lesmana, Cokorda Bagus Jaya; Goldberg, Elkhonon
作者单位：Rice University; Universitas Udayana
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作者：Kolosova, Olga; Zgadzay, Yury; Stetsenko, Artem; Sukhinina, Anastasia P.; Atamas, Anastasia; Validov, Shamil; Rogachev, Andrey; Usachev, Konstantin; Jenner, Lasse; Dmitriev, Sergey E.; Yusupova, Gulnara; Guskov, Albert; Yusupov, Marat
作者单位：Institut National de la Sante et de la Recherche Medicale (Inserm); Universites de Strasbourg Etablissements Associes; Universite de Strasbourg; University of Groningen; Lomonosov Moscow State University; Lomonosov Moscow State University; Kazan Federal University; Joint Institute for Nuclear Research - Russia
摘要：Nonsense mutations are associated with numerous and diverse pathologies, yet effective treatment strategies remain elusive. A promising approach to combat these conditions involves the use of aminoglycosides, particularly in combination with stop-codon read-through enhancers, for developing drugs that can rescue the production of full-length proteins. Using X-ray crystallography and single-particle cryo-EM, we obtained structures of the eukaryotic ribosome in complexes with several aminoglycos...
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作者：Cheng, An-Po; Huang, Lihong; Oberkofler, Lorenz; Johnson, Nathan R.; Glodeanu, Adrian-Stefan; Stillman, Kyra; Weiberg, Arne
作者单位：University of Munich; Universidad Mayor; University of Hamburg; Guangzhou Medical University
摘要：Argonaute (AGO) proteins bind to small RNAs to induce RNA interference (RNAi), a conserved gene regulatory mechanism in animal, plant, and fungal kingdoms. Small RNAs of the fungal plant pathogen Botrytis cinerea were previously shown to translocate into plant cells and to bind to the host AGO, which induced cross-kingdom RNAi to promote infection. However, the role of pathogen AGOs during host infection stayed elusive. In this study, we revealed that members of fungal plant pathogen B. cinere...
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作者：Djermoun, Sarah; Rode, Daniel K. H.; Jimenez-Siebert, Eva; Netter, Niklas; Lesterlin, Christian; Drescher, Knut; Bigot, Sarah
作者单位：Universite Lyon 1; Centre National de la Recherche Scientifique (CNRS); Institut National de la Sante et de la Recherche Medicale (Inserm); University of Basel
摘要：Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that significantly contributes to the dissemination of antibiotic resistance among bacteria. While the molecular mechanisms of conjugation have been extensively studied, our understanding of plasmid transfer dynamics within spatially structured bacterial communities and the influence of community architecture on plasmid dissemination remains limited. In this study, we use live-cell fluorescence microscopy to investig...
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作者：Makinen, Jussi; Ellis, Emilie E.; Antao, Laura H.; Davrinche, Andrea; Laine, Anna-Liisa; Saastamoinen, Marjo; Conenna, Irene; Hallforsa, Maria; Santangeli, Andrea; Kaarlejarvi, Elina; Heliolae, Janne; Huikkonen, Ida-Maria; Kuussaari, Mikko; Leinonen, Reima; Lehikoinen, Aleksi; Poyry, Juha; Suuronen, Anna; Salemaa, Maija; Tonteri, Tiina; Vuorio, Kristiina M.; Skjelbred, Birger; Jarvinen, Marko; Drakare, Stina; Carvalho, Laurence; Welk, Erik; Seidler, Gunnar; Vangansbeke, Pieter; Malis, Frantisek; Hedl, Radim; Auffret, Alistair G.; Plue, Jan; De Frenne, Pieter; Kalwij, Jesse M.; Vanhatalo, Jarno; Roslin, Tomas
作者单位：University of Helsinki; Finnish Environment Institute; University of Turku; Consejo Superior de Investigaciones Cientificas (CSIC); ATTITUS Educacao; University of Helsinki; Natural Resources Institute Finland (Luke); Norwegian Institute for Water Research (NIVA); Swedish University of Agricultural Sciences; Martin Luther University Halle Wittenberg; Ghent University; Technical University Zvolen; Czech Academy of Sciences; Institute of Botany of the Czech Academy of Sciences; Palacky University Olomouc; Swedish University of Agricultural Sciences; Swedish University of Agricultural Sciences; Helmholtz Association; Karlsruhe Institute of Technology; University of Johannesburg; University of Helsinki; University of Helsinki
摘要：Globally, rising temperatures are increasingly favoring warm-affiliated species. Although changes in community composition are typically measured by the mean temperature affinity of species (the community temperature index, CTI), they may be driven by different processes and accompanied by shifts in the diversity of temperature affinities and breadth of species thermal niches. To resolve the pathways to community warming in Finnish flora and fauna, we examined multidecadal changes in the domin...
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作者：Piacentino, Michael L.; Hutchins, Erica J.; Andrews, Cecelia J.; Bronner, Marianne E.
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作者：Wang, Yong; Wang, Junjie; Yao, Ge; Fan, Zheyong; Granato, Enzo; Kosterlitz, Michael; Ala-Nissila, Tapio; Car, Roberto; Sun, Jian
作者单位：Nanjing University; Nanjing University; Collaborative Innovation Center of Advanced Microstructures (CICAM); Princeton University; Bohai University; Instituto Nacional de Pesquisas Espaciais (INPE); Brown University; Aalto University; Loughborough University; Princeton University
摘要：The nature of solid phases and cross-over of order-disorder phase transitions from twodimensional (2D) layers to three-dimensional (3D) bulk in confined atomic systems remain largely unexplained. To this end, we consider noble gases and aluminum confined between graphene sheets at different pressures and temperatures. Using crystal structure search methods and molecular dynamics based on machine-learned potentials with quantum-mechanical accuracy, we identify structures of multilayer confined ...
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作者：Foffi, Erica; Rugolo, Francesco; Ramamurthy, Nisha; Haight, Jillian; Helke, Simone; You-Ten, Annick; Tobin, Chantal; Jafari, Soode Moghadas; Elia, Andrew J.; Berger, Thorsten; Candi, Eleonora; Melino, Gerry; Mak, Tak W.
作者单位：University of Toronto; University Health Network Toronto; Princess Margaret Cancer Centre; University of Toronto; University Health Network Toronto; Princess Margaret Cancer Centre; IRCCS Istituto Dermopatico dell'Immacolata (IDI); University of Rome Tor Vergata; University of Hong Kong; University of Toronto; University of Toronto
摘要：Chronic inflammatory skin disorders are characterized by keratinocyte hyperproliferation and hyperactivation as well as immune cell infiltration. We investigated whether immune cell-derived acetylcholine (ACh) is a modulator of skin inflammation in mice. Here, we identify skin epithelial B cells as a key source of ACh that damps down inflammation. We used imiquimod (IMQ) to induce inflammatory skin disease (ISD) in mice lacking ACh production specifically in B cells (ChATfl/fl;Mb1-Cre mice). I...
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作者：Fisher, Nicole M.; von Zastrow, Mark
作者单位：University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco
摘要：Many GPCRs initiate a second phase of G protein-mediated signaling from endosomes. This inherently requires the GPCR to increase cognate G protein activity on the endosome surface. Gs-coupled GPCRs are thought to achieve this by internalizing and mediating a second round of allosteric coupling to G proteins on the endosome membrane. Here, we provide evidence that the mu- opioid receptor (MOR), a Gi-coupled GPCR, is able to increase endosomal G protein activity in a different way. Leveraging co...
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作者：Pharkjaksu, Sujiraphong; Cai, Hongyi; Walter, Peter J.; Chang, Yun C.; Kwon-Chung, Kyung J.
作者单位：National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID); National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
摘要：UDP- glucuronic acid (UDP- GlcUA) is a nucleotide sugar essential for various biological processes in many organisms, and its excess within the cell can disrupt cellular functions. In Cryptococcus, mutations in the UXS1 gene which encodes an enzyme responsible for converting UDP- GlcUA into UDP- xylose, result in excessive accumulation of UDP- GlcUA and confer resistance to the antifungal drug 5- fluorocytosine. Here, we demonstrate that elevation of UDP- GlcUA affects several cellular process...
