Fungal Argonaute proteins act in bidirectional cross-kingdom RNA interference during plant infection

• 成果类型: Article
• 署名作者: Cheng, An-Po; Huang, Lihong; Oberkofler, Lorenz; Johnson, Nathan R.; Glodeanu, Adrian-Stefan; Stillman, Kyra; Weiberg, Arne
• 署名单位: University of Munich; Universidad Mayor; University of Hamburg; Guangzhou Medical University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2422756122
• 发表日期: 2025-04-29
• 页码: e2422756122
• 关键词: Argonaute; cross-kingdom RNA interference; small RNA; plant-fungal interaction; botrytis-cinerea; gene; pathways; DEFENSE

摘要

Argonaute (AGO) proteins bind to small RNAs to induce RNA interference (RNAi), a conserved gene regulatory mechanism in animal, plant, and fungal kingdoms. Small RNAs of the fungal plant pathogen Botrytis cinerea were previously shown to translocate into plant cells and to bind to the host AGO, which induced cross-kingdom RNAi to promote infection. However, the role of pathogen AGOs during host infection stayed elusive. In this study, we revealed that members of fungal plant pathogen B. cinerea BcAGO family contribute to plant infection. BcAGO1 binds to both fungal and plant small RNAs during infection and acts in bidirectional cross-kingdom RNAi, from fungus to plant and vice versa. BcAGO2 also binds fungal and plant small RNAs but acts independent from BcAGO1 by regulating distinct genes. Nevertheless, BcAGO2 is important for infection, as it is required for effective pathogen small RNA delivery into host cells and fungal induced cross-kingdom RNAi. Providing these mechanistic insights of pathogen AGOs promises to improve RNAi-based crop protection strategies.