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作者：Cheng, Holly H.; Roggeveen, James V.; Wang, Huan; Stone, Howard A.; Shi, Zheng; Brangwynne, Clifford P.
作者单位：Princeton University; Rutgers University System; Rutgers University New Brunswick; Princeton University; Princeton University; Princeton University; Howard Hughes Medical Institute
摘要：The nucleolus is a multiphasic biomolecular condensate that facilitates ribosome biogenesis, a complex process involving hundreds of proteins and RNAs. The proper execution of ribosome biogenesis likely depends on the material properties of the nucleolus. However, these material properties remain poorly understood due to the challenges of in vivo measurements. Here, we use micropipette aspiration (MPA) to directly characterize the viscoelasticity and interfacial tensions of nucleoli within tra...
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作者：Lopez-Pereza, Mario; Balasubramanian, Deepak; Lopez, Alicia Campos-; Crist, Cole; Grant, Trudy - Ann; Haro-Moreno, Jose M.; Zaragoza-Solas, Asier; Almagro-Moreno, Salvador
作者单位：Universidad Miguel Hernandez de Elche; St Jude Children's Research Hospital
摘要：The underlying factors that lead to specific strains within a species to emerge as human pathogens remain mostly enigmatic. The diarrheal disease cholera is caused by strains from a phylogenetically confined group within the Vibrio cholerae species, the pandemic cholera group (PCG), making it an ideal model system to tackle this puzzling phenomenon. Comprehensive analyses of over 1,840 V. cholerae genomes, including environmental isolates from this study, reveal that the species consists of el...
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作者：Stiller, Josefin
作者单位：University of Copenhagen
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作者：Clark, Cory J.; Jussim, Lee; Frey, Komi; Stevens, Sean T.; al-Gharbi, Musa; Aquino, Karl; Bailey, J. Michael; Barbaro, Nicole; Baumeister, Roy F.; Bleske-Rechek, April; Buss, David; Ceci, Stephen; Giudice, Marco Del; Ditto, Peter H.; Forgas, Joseph P.; Geary, David C.; Geher, Glenn; Haider, Sarah; Honeycutt, Nathan; Joshi, Hrishikesh; Krylov, Anna I.; Loftus, Elizabeth; Loury, Glenn; Lu, Louise; Macy, Michael; Martin, Chris C.; McWhorter, John; Miller, Geoffrey; Paresky, Pamela; Pinker, Steven; Reilly, Wilfred; Salmon, Catherine; Stewart-Williams, Steve; Tetlock, Philip E.; Williams, Wendy M.; Wilson, Anne E.; Winegard, Bo M.; Yancey, George; von Hippel, William
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作者：Ornes, Stephen
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作者：Siqueira-Batista, Rodrigo; Gomez, Felipe
作者单位：Universidade Federal de Vicosa; Consejo Superior de Investigaciones Cientificas (CSIC)
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作者：Whetsel, Charles W.; Levine, Joel S.; Hoffman, Stephen J.; Antonsen, Erik L.
作者单位：California Institute of Technology; National Aeronautics & Space Administration (NASA); NASA Jet Propulsion Laboratory (JPL); Aerospace Corporation - USA; Massachusetts General Hospital; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital
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作者：Kalutskii, Maksim; Grubmueller, Helmut; Volkov, Vladimir A.; Igaev, Maxim
作者单位：University of London; Queen Mary University London; University of Dundee
摘要：Microtubules are dynamic cytoskeletal polymers that add and lose tubulin dimers at their ends. Microtubule growth, shortening, and transitions between them are linked to GTP hydrolysis. Recent evidence suggests that flexible tubulin protofilaments at microtubule ends adopt a variety of shapes, complicating structural analysis using conventional techniques. Therefore, the link between GTP hydrolysis, protofilament structure and microtubule polymerization state is poorly understood. Here, we inv...
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作者：Ota, Naruhisa; Davies, Christopher W.; Kang, Jing; Yan, Donghong; Scherl, Alexis; Wong, Anne; Cook, Ryan; Tao, Xun; Dunlap, Debra; Klabunde, Sha; Mantik, Priscilla; Mohanan, Vishnu; Lin, WeiYu; McBride, Jacqueline; Sadekar, Shraddha; Storek, Kelly M.; Lupardus, Patrick; Ye, Zhengmao; Wallweber, Heidi Ackerly; Kiefer, James R.; Xu, Min; Chan, Pamela; Nagapudi, Karthik; Xi, Tangshen; Koerber, James T.
作者单位：Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech; Roche Holding; Roche Holding USA; Genentech
摘要：Interleukin (IL)- 23 is a validated therapeutic target in inflammatory bowel disease. While antibodies targeting IL23 demonstrate clinical efficacy, they face challenges such as high costs, safety risks, and the necessity of parenteral administration. Here, we present a workflow to simultaneously enhance the affinity and protease stability of an anti- IL23R VHH employs both CDR and framework residues to achieve picomolar affinity for IL23R. The engineered VHH remains stable for over 8 h in int...
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作者：Peter, Sandra; Riemer, Kai; West, Jevin D.
作者单位：University of Sydney; University of Washington; University of Washington Seattle
摘要：A growing body of research suggests that the recent generation of large language model (LLMs) excel, and in many cases outpace humans, at writing persuasively and empathetically, at inferring user traits from text, and at mimicking human-like conversation believably and effectively-without possessing any true empathy or social understanding. We refer to these systems as anthropomorphic conversational agents to aptly conceptualize the ability of LLM-based systems to mimic human communication so...
