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作者：Cheong, Mi Cheong; Mackowiak, Bryan; Kim, Hyung Bum; Hernandez, Genaro; Nandu, Tulip; Vale, Kevin; Zhang, Yuan; Zacharias, Lauren G.; Mathews, Thomas P.; Gao, Bin; Kraus, W. Lee; Kliewer, Steven A.; Mangelsdorf, David J.
作者单位：University of Texas System; University of Texas Southwestern Medical Center; National Institutes of Health (NIH) - USA; NIH National Institute on Alcohol Abuse & Alcoholism (NIAAA); University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; Howard Hughes Medical Institute; University of Texas System; University of Texas Southwestern Medical Center
摘要：Ethanol rapidly stimulates the liver to synthesize the hormone fibroblast growth factor 21 (FGF21), which then acts on the brain to elicit a multifaceted protective response. We show that in mice, this induction of FGF21 occurs at the level of gene transcription and is regulated by two byproducts of ethanol metabolism, glycerol-3-phosphate (G3P) and acetyl-CoA. Using cell-based reporter and thermal shift binding assays, we show that G3P binds to a conserved domain and activates the transcripti...
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作者：Li, Shufen; Li, Hao; Zhou, Zhenxing; Ye, Meidi; Wang, Yifei; Li, Wenqin; Guan, Zhenqiong; Guan, Zihan; Zhang, Chongtao; Zhang, Yulan; Liu, Wei; Peng, Ke
作者单位：Chinese Academy of Sciences; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Academy of Military Medical Sciences - China
摘要：Necroptosis is an inflammatory programmed cell death pathway triggered by RIPK3 activation through one of the upstream RHIM-domain-containing proteins including RIPK1, TRIF, and ZBP1. Whether necroptosis can be activated independent of the upstream signaling pathways leading to inflammatory pathogenesis remains ambiguous. Here, we revealed a mechanism in which a viral protein mediates direct RIPK3 activation resulting in severe inflammatory pathogenesis in patients. The nonstructural protein N...
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作者：Vergish, Satyam; Huang, Xiaoen; Zhang, Guiyun; Franceschi, Beatriz de Toledo; Li, Jian-Liang; Wu, Xiao-Xia; Nuraj, Joana; Huguet-Tapia, Jose C.; Parajuli, Apekshya; Chen, Xiuhua; Shekhar, Ritu; Liu, Dali; Xiao, Wu-Ming; Dou, Shijuan; Liu, Guo-zhen; Goss, Erica M.; Pi, Liya; Chen, Sixue; Koch, Karen E.; Song, Wen- Yuan
作者单位：State University System of Florida; University of Florida; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS); State University System of Florida; University of Florida; Hebei Agricultural University; State University System of Florida; University of Florida; Tulane University; University of Mississippi; State University System of Florida; University of Florida; State University System of Florida; University of Florida; State University System of Florida; University of Florida; Yangzhou University; State University System of Florida; Florida International University; Heilongjiang University; South China Agricultural University
摘要：To maintain growth and to successfully reproduce, organisms must protect key functions in specific tissues, particularly when countering pathogen invasion using internal defensive proteins that may disrupt their own developmental processes. The rice immune receptor XA21 confers race-specific resistance against Xanthomonas oryzae pv. oryzae, which causes the deadly disease bacterial leaf blight. Here, we demonstrate that XA21 is cleaved by the rhomboid-like protease OsRBL3b, likely within its t...
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作者：Wang, Xixian; Wang, Sen; Diao, Zhidian; Hou, Xibao; Gong, Yanhai; Sun, Qing; Zhang, Jiaping; Ren, Lihui; Li, Yuandong; Ji, Yuetong; Shen, Wei; Yin, Yifeng; Huang, Shi; Song, Xiaojin; Cui, Qiu; Feng, Yingang; Xu, Jian; Ma, Bo
作者单位：Chinese Academy of Sciences; Qingdao Institute of Bioenergy & Bioprocess Technology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; University of Hong Kong
摘要：A full spontaneous single-cell Raman spectrum captures the metabolic phenome in a label-free and noninvasive manner. However, Raman-activated cell sorting (RACS) of rare target cells from highly heterogeneous systems has remained largely conceptual. Here, we present a positive dielectrophoresis-induced deterministic lateral displacement (pDEP-DLD)-based RACS (pDEP-DLD-RACS), in which a modulated pDEP-DLD force is applied to focus, trap, and functionally sort fast-moving single cells in a wide ...
