36期

最新文章

• The molecular structure of the master growth regulator in plants emerges into the spotlight AI翻译
  作者：Yoshida, Hideki; Matsuoka, Makoto
  作者单位：Fukushima University
• Manipulating anthracyclines for deeper tissue penetration and implications for glycolytic tissues AI翻译
  作者：Abels, Erik R.; ter Linden, Esther; Rohling, Jos H. T.; Voortman, Lennard M.; Janssen, Lennert; Hornsveld, Marten; Overkleeft, Herman S.; van der Zanden, Sabina Y.; Broekman, Marike L. D.; Neefjes, Jacques
  作者单位：Leiden University - Excl LUMC; Leiden University; Leiden University Medical Center (LUMC); Leiden University; Leiden University Medical Center (LUMC); Leiden University - Excl LUMC; Leiden University - Excl LUMC; Leiden University; Leiden University - Excl LUMC; Leiden University; Leiden University Medical Center (LUMC); Haaglanden Medical Center
  摘要：How drugs penetrate tissues is poorly understood yet important, since drugs that fail to reach their target will be ineffective. We followed the fate of anthracycline cancer drugs at high resolution by exploiting their intrinsic fluorescence. In a cell-based spheroid model, the soluble compound fluorescein penetrates the entire spheroid, unlike hydrophobic fluorescent lipids, which only enter the outermost cell layer. Anthracyclines have intermediate hydrophobicity. They enter the nucleus of a...
• Resurfacing promotes antibacterial activity of a lipid A-binding nanobody AI翻译
  作者：O'Donnell, Angela C.; Wang, Xun; Kaderabkova, Nikol; Groover, Kyra E.; Perez, Bethany C.; Helms, Amanda; Brodbelt, Jennifer S.; Mavridou, Despoina A. I.; Davies, Bryan W.
  作者单位：University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; United States Department of Energy (DOE); Lawrence Livermore National Laboratory
  摘要：Nanobodies have been pursued as candidates for antimicrobial design due to their small size and versatile binding capacities, but direct antibacterial activity of a nano-body has yet to be described. Here, we employed a bacterial surface display platform to screen a synthetic library of nanobody variants for antimicrobial potential. We identified a candidate that binds the essential lipid A component of gram-negative lipopolysaccharide. Nonetheless, this nanobody required a weakened outer memb...
• Subaerial oxidative uranium mobilization at the culmination of the Great Oxidation Event AI翻译
  作者：Bauer, Ann M.; Li, Weiqiang; Rybacki, Kyle S.; Roden, Eric E.; Kump, Lee R.; Johnson, Clark M.
  作者单位：University of Wisconsin System; University of Wisconsin Madison; National Aeronautics & Space Administration (NASA); Nanjing University; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park
  摘要：Redox-sensitive elements figure prominently in studies of the evolution of Earth's surface redox state, including the first major rise in atmospheric O-2, the Paleoproterozoic Great Oxidation Event. Most Precambrian rocks endured multistage tectonothermal histories, however, adding ambiguity to interpretation of their chemistry. Here, we apply U-Th-Pb isotope geochronology to the highly oxidized similar to 2.06 Ga Kuetsjarvi Volcanic Formation, Pechenga Greenstone Belt, Russia, to constrain th...
• Testis expressed 50 is essential for maintaining sperm acrosome integrity during epididymal transit AI翻译
  作者：Haga, Saori; Nozawa, Kaori; Abbasi, Ferheen; Kent, Katarzyna; Nagasawa, Naoko; Endo, Tsutomu; Miyata, Haruhiko; Ikawa, Masahito; Matzuk, Martin M.; Fujihara, Yoshitaka
  作者单位：National Cerebral & Cardiovascular Center - Japan; University of Osaka; University of California System; University of California Davis; Baylor College of Medicine; University of Tokyo; University of Osaka; University of Tokyo
  摘要：In mammals, sperm formation is completed in the seminiferous tubules within the testis, and sperm maturation occurs during the epididymal transit of the spermatozoa. Sperm morphology drastically changes when abnormal spermatozoa migrate from the testis to the epididymis. Detailed molecular mechanisms for sperm survival in the epididymis have not been determined yet. Globozoospermia is a cause of male infertility and is characterized by round-headed spermatozoa without acrosomes, an abnormal sp...
• Procollagen IIA mediates positive feedback control of the mouse cardiogenic transcriptional network AI翻译
  作者：Leung, Alan W.; Wong, Sandra Y.; Zhang, Janet C.; Leung, Keith K. H.; Dung, Nelson W. F.; Shang, Catherine A.; Prall, Owen W.; Nim, Hieu T.; See, Michael; Ramialison, Mirana; Chan, Danny; Mohun, Timothy J.; Harvey, Richard P.; Tam, Patrick P. L.; Cheah, Kathryn S. E.
  作者单位：University of Hong Kong; University of Hong Kong; Francis Crick Institute; Victor Chang Cardiac Research Institute; University of Melbourne; Murdoch Children's Research Institute; University of Melbourne; Monash University; Australian Regenerative Medicine Institute; Monash University; University of New South Wales Sydney; University of New South Wales Sydney; University of Sydney; Children's Medical Research Institute - Australia; University of Sydney
  摘要：Cardiogenesis relies on the integrated interplay between cardiac transcription factors and signaling pathways. Here, we uncover a role for type IIA procollagen (IIA), an extracellular matrix (ECM) protein encoded by an alternatively spliced Col2a1 transcript, encoding a N-terminal cysteine-rich domain, as a critical regulator in a cardiac gene regulatory feedback loop. The cysteine-rich domain of IIA protein was previously reported to interact with bone morphogenetic proteins (BMPs) and transf...
• De novo design of protein binders to stabilize monomeric TDP-43 and inhibit its pathological aggregation AI翻译
  作者：Sun, Gangyu; Li, Xiang; Hu, Jiaojiao; Yang, Tianbin; Liu, Cong; Wang, Zhizhi; Li, Dan; Xu, Wenqing
  作者单位：ShanghaiTech University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Chinese Academy of Sciences; Shanghai Institute of Organic Chemistry, CAS; Fudan University
  摘要：Pathological aggregation of transactive response DNA binding protein of 43 kDa (TDP-43), primarily driven by its low-complexity domain, is closely associated with various neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Despite the therapeutic potential of preventing TDP-43 aggregation, no effective small molecule or biomacromolecule therapeutics have been successfully developed so far. Here, we introduce a protein design s...
• Nonlinear memory in cell-division dynamics across species AI翻译
  作者：Zhang, Shijie; Fei, Chenyi; Dunkel, Jorn
  作者单位：Massachusetts Institute of Technology (MIT)
  摘要：Regulation of cell growth and division is essential to achieve cell-size homeostasis. Recent advances in imaging technologies, such as mother machines for bacteria or yeast, have allowed long-term tracking of cell-size dynamics across many generations, and thus have brought major insights into the mechanisms underlying cell-size control. However, understanding the governing rules of cell growth and division within a quantitative dynamical-systems framework remains a major challenge. Here, we i...
• Action at a distance: The remarkable coupling of CO2 uptake to electron transfer in specialized cyanobacterial NDH-1 complexes AI翻译
  作者：Zhang, Zhifen; Zhang, Minquan; Burnap, Robert L.
  作者单位：Oklahoma State University System; Oklahoma State University - Stillwater
  摘要：Cyanobacteria achieve highly efficient photosynthesis using a CO2-concentrating mechanism relying on specialized Type I (NDH-1) complexes. Among these, NDH-1(3) and NDH-1(4) catalyze redox-coupled hydration of CO2 to bicarbonate, supporting carbon fixation in carboxysomes. The mechanism of coupling electron transfer to CO2-hydration by these variant NDH-1 complexes remains unknown. We engineered a Synechococcus PCC7942 strain that expresses exclusively the high flux/low affinity NDH-1(4) compl...
• Using bioinformatics for identifying and plugging metabolic pathway holes AI翻译
  作者：Galperin, Michael Y.
  作者单位：National Institutes of Health (NIH) - USA; NIH National Library of Medicine (NLM); Division of Intramural Research (DIR)