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作者：Yoneyama, Yosuke; Zhang, Ran-Ran; Maezawa, Mari; Masaki, Hideki; Kimura, Masaki; Cai, Yuqi; Adam, Mike; Parameswaran, Sreeja; Mizuno, Naoaki; Bhadury, Joydeep; Maezawa, So; Ochiai, Hiroshi; Nakauchi, Hiromitsu; Potter, S. Steven; Weirauch, Matthew T.; Takebe, Takanori
作者单位：University of Osaka; Cincinnati Children's Hospital Medical Center; Cincinnati Children's Hospital Medical Center; Cincinnati Children's Hospital Medical Center; Cincinnati Children's Hospital Medical Center; University System of Ohio; University of Cincinnati; Stanford Medicine; Stanford University; Tokyo University of Science; Kyushu University; University System of Ohio; University of Cincinnati; Cincinnati Children's Hospital Medical Center
摘要：Intercellular transmission of messenger RNA (mRNA) is being explored in mammalian species using immortal cell lines. Here, we uncover an intercellular mRNA transfer phenomenon that allows for the adaptation and reprogramming of human primed pluripotent stem cells (hPSCs). This process is induced by the direct cell contact- mediated coculture with mouse embryonic stem cells under the condition impermissible for primed hPSC culture. Mouse- derived mRNA contents are transmitted into adapted hPSCs...
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作者：Zhang, Jiaojiao; Xu, Jiayi; Wang, Xinman; Liu, Ying; Li, Shuangtao; Zhang, Jialong; He, Lingfeng; Guo, Luqin; Li, Chonghua; Li, Xin - Xu; Guo, Yang - Dong; Zhang, Na
作者单位：China Agricultural University; Hebei Normal University of Science & Technology; Shanghai Academy of Agricultural Sciences; Beijing Academy of Agriculture & Forestry Sciences (BAAFS)
摘要：Light serves as an energy source for cell division and expansion during fruit development. Cell expansion significantly influences fruit size and is closely linked to endoreduplication, a unique cell cycle variation characterized by DNA replication without cytokinesis. Paradoxically, under conditions of ample photosynthates, light signaling suppresses cell expansion. The intricate regulation of endoreduplication in response to light signaling during fruit development has remained an intriguing...
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作者：Biswas, Nilima; Mori, Tommaso; Nagaraj, Naresh Kumar Ragava Chetty; Xin, Hong; Diemer, Tanja; Li, Pin; Su, Yongxuan; Piermarocchi, Carlo; Ferrara, Napoleone
作者单位：University of California System; University of California San Diego; University of California System; University of California San Diego; Michigan State University; University of California System; University of California San Diego; China Pharmaceutical University
摘要：We hypothesized that a strategy employing tissue- specific endothelial cells (EC) might facilitate the identification of tissue- or organ- specific vascular functions of ubiquitous metabolites. An unbiased approach was employed to identify water- soluble small molecules with mitogenic activity on choroidal EC. We identified adenosine diphosphate (ADP) as a candidate, following biochemical purification from mouse EL4lym- phoma extracts. ADP stimulated the growth of bovine choroidal EC (BCEC) an...
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作者：Mok, Stephen; Liu, Huey; Cobanoglu, Didem Agac; Anang, Nana- Ama A. S.; Mancuso, James J.; Wherry, E. John; Allison, James P.
作者单位：University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; University of Pennsylvania; University of Texas System; UTMD Anderson Cancer Center
摘要：The effects of T cell differentiation arising from immune checkpoint inhibition targeting cytotoxic T lymphocyte-associated antigen 4 (CTLA- 4) and programmed cell death protein 1 (PD- 1) on the immunological memory response remain unclear. Our investigation reveals that memory T cells generated by anti- CTLA- 4 exhibit greater expansion, cytokine preserves more T cell factor- 1 (TCF- 1)+ T cells during priming, while anti- PD- 1 leads to using conditional Tcf7- or Tox- knockout mice highlight...
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作者：Mazigi, Ohan; Langley, David B.; Henry, Jake Y.; Burnett, Deborah L.; Sobti, Meghna; Walker, GregoryJ.; Rouet, Romain; Balachandran, Harikrishnan; Lenthall, Helen; Jackson, Jennifer; Ubiparipovic, Stephanie; Schofield, Peter; Brown, Simon H. J.; Schulz, Sebastian R.; Hoffmann, Markus; Poehlmann, Stefan; Post, Jeffrey; Martinello, Marianne; Ahlenstiel, Golo; Kelleher, Anthony; Rawlinson, William D.; Turville, Stuart G.; Bull, Rowena A.; Stewart, Alastair G.; Jaeck, Hans-Martin; Goodnow, Christopher C.; Christ, Daniel
作者单位：Garvan Institute of Medical Research; University of New South Wales Sydney; University of New South Wales Sydney; Victor Chang Cardiac Research Institute; University of New South Wales Sydney; Prince of Wales Hospital (POWH); University of New South Wales Sydney; Kirby Institute; University of Wollongong; University of Wollongong; University of Erlangen Nuremberg; University of Erlangen Nuremberg; Leibniz Association; Deutsches Primatenzentrum (DPZ); University of Gottingen; University of New South Wales Sydney; NSW Health; Blacktown & Mount Druitt Hospital
摘要：The emergence of SARS-CoV-2 variants of concern (VOCs) has greatly diminished the neutralizing activity of previously FDA- approved monoclonal antibodies (mAbs), including that of antibody cocktails and of first- generation broadly neutralizing antibodies such as S309 (Sotrovimab). In contrast, antibodies targeting cryptic conformational epitopes of the receptor binding domain (RBD) have demonstrated broad activity against emerging variants, but exert only moderate neutralizing activity, which...
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作者：Wang, Tongtong; Huang, Wenze; Xu, Kui; Sun, Yitong; Zhang, Qiangfeng Cliff; Yan, Chuangye; Li, Zhangqiang; Yan, Nieng
作者单位：Tsinghua University; Shenzhen Medical Academy of Research & Translation (SMART); Shenzhen Bay Laboratory
摘要：Despite the recent breakthrough in structure determination and prediction of proteins, the structural investigation of carbohydrates remains a challenge. Here, we report the cryo-EM analysis of a glycofibril found in the freshwater in the Tsinghua Lotus Pond. The fibril, which we name TLP-4, is made of a linear chain of tetrapeptide repeats coated with >4 nm thick glycans. In each repeat, two glycans are O- linked to a 3,4- dihydroxyproline and another glycan attaches to the adjacent Ser or Th...
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作者：Zhou, Songsong; Rappe, Andrew M.
作者单位：University of Pennsylvania
摘要：Ferroelectric hafnia exhibits promising robust polarization and silicon compatibility for ferroelectric devices. Unfortunately, it suffers from difficult polarization switching. Methods to enable easier polarization switching are needed, and the underlying reason for this switching difficulty is not understood. Here, we investigated the 180 degrees domain walls of hafnia and their motion through nucleation. We found that the domains of multiple-order parameter hafnia possess complicated three-...
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作者：Chen, Gongwen; Jun, Ji Hyung; Wijshake, Tobias; Kwarteng, Edward O.; Li, Yunyang; Yuan, Minwei; Rose, Joseph; Li, Shan; Cobb, Sarah; Serpa, Willow; Folger, Brayden; Li, Yafeng; Li, Li; Chen, Weina; Collins, James J.; Wang, Jipeng; Agathocleous, Michali
作者单位：Fudan University; University of Texas System; University of Texas Southwestern Medical Center; University of Texas System; University of Texas Southwestern Medical Center; Inner Mongolia University; University of Texas System; University of Texas Southwestern Medical Center; Texas Tech University System; Texas Tech University; University of Texas System; University of Texas Southwestern Medical Center; Howard Hughes Medical Institute
摘要：The ability to synthesize essential molecules is sometimes lost in evolution. A classic example is ascorbate (vitamin C), which is synthesized in most animals by L-gulonolactone oxidase (GULO), an enzyme lost multiple independent times in animal evolution. This event is thought to be evolutionarily neutral; however, GULO-deficient animals including humans need to obtain ascorbate from their diet and are prone to ascorbate deficiency and scurvy. We therefore hypothesized that this disadvantage ...
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作者：Cifarelli, Vincenza
作者单位：Saint Louis University
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作者：Zhang, Yangyi; Ren, He; Ma, Chaobing; Shi, Changhong; Yang, Ruigang; Wang, Chenxi; Feng, Pengfei; Zhang, Bo; Liu, Chenyu; Niu, Zubiao; Yang, Yalan; Zheng, You; Sun, Zhuoran; Zhang, Ying; Zhang, Shinan; Melino, Gerry; Huang, Hongyan; Sun, Qiang
作者单位：Capital Medical University; Academy of Military Medical Sciences - China; Chinese Academy of Medical Sciences - Peking Union Medical College; Capital Medical University; Air Force Medical University; University of Rome Tor Vergata
摘要：Despite great success in certain cancers, immunotherapy made little progress in treating immune cold tumors, largely attributed to an immune-suppressive tumor microenvironment with elusive mechanisms. Here, we report in prostate cancer cells a positive feedback loop driven byphosphoserine aminotransferase 1 (PSAT1) that could be targeted to render effective cytotherapy by natural killer (NK) cells. In the loop, PSAT1 increases Y-box binding protein 1 (YBX1) phosphorylation by microtubule affin...
