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作者：Wang, Yinhang; Wang, Xingyu; Alabdullatif, Salam; Homma, Sachiko T.; Alekseyev, Yuriy O.; Zhou, Lan
作者单位：Boston University; Boston University; Boston University
摘要：Infiltrating macrophages contribute to muscle dystrophic changes in Duchenne musblood Ly6Chi inflammatory monocyte recruitment. This is accompanied by transient improvement of muscle damage, fibrosis, and regeneration. The benefit, however, is lost after the expansion of intramuscular Ly6Clo macrophages. To address the mechanisms underlying the Ly6Clo macrophage expansion, we compared mdx5cv/Nur77-/- and mdx5cv/ Ccr2-/-/Nur7-/- mice with mdx5cv and mdx5cv/Ccr2-/- mice, respectively, and found ...
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作者：Domenzain, Ivan; Lu, Yao; Wang, Haoyu; Shi, Junling; Lu, Hongzhong; Nielsen, Jens
作者单位：Chalmers University of Technology; Chalmers University of Technology; Northwest A&F University - China; Shanghai Jiao Tong University; Chinese Academy of Sciences; Dalian Institute of Chemical Physics, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Northwestern Polytechnical University
摘要：Development of efficient cell factories that can compete with traditional chemical production processes is complex and generally driven by case- specific strategies, based on the product and microbial host of interest. Despite major advancements in the field of metabolic modeling in recent years, prediction of genetic modifications for increased production remains challenging. Here, we present a computational pipeline that leverages the concept of protein limitations in metabolism for predicti...
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作者：Chou, Yu - Shu; Logeswaran, Dhenugen; Chow, Chi - Nga; Dunn, Phoebe L.; Podlevsky, Joshua D.; Liu, Tianxiang; Akhter, Khadiza; Chen, Julian J. - L.
作者单位：Arizona State University; Arizona State University-Tempe; Arizona State University; Arizona State University-Tempe; United States Department of Energy (DOE); Sandia National Laboratories
摘要：Telomerase elongates telomeres to maintain chromosome stability in most eukaryotes. Despite extensive studies across eukaryotic kingdoms, the telomerase holoenzyme in arthropods remains poorly understood. In this study, we purify the telomerase ribonucleoprotein complex from the lepidopteran insect Spodoptera frugiperda (fall army- worm) and identify a copurified 135- nucleotide telomerase RNA (TR) component. This miniature S. frugiperda TR (sfTR), the smallest TR known to date, retains a univ...
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作者：Alliston, Spencer P.; Dames, Chris; Powell-Palm, Matthew J.
作者单位：University of California System; University of California Berkeley; Texas A&M University System; Texas A&M University College Station; Texas A&M University System; Texas A&M University College Station; Texas A&M University System; Texas A&M University College Station
摘要：Predictive synthesis of aqueous organic solutions with desired liquid-solid phase equilibria could drive progress in industrial chemistry, cryopreservation, and beyond, but is limited by the predictive power of current solution thermodynamics models. In particular, few analytical models enable accurate liquidus and eutectic prediction based only on bulk thermodynamic properties of the pure components, requiring instead either direct measurement or costly simulation of solution properties. this...
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作者：Di Trani, Justin M.; Yu, Jiacheng; Courbon, Gautier M.; Rodriguez, Ana Paula Lobez; Cheung, Chen-Yi; Liang, Yingke; Coupland, Claire E.; Bueler, Stephanie A.; Cook, Gregory M.; Brzezinski, Peter; Rubinstein, John L.
作者单位：University of Toronto; Hospital for Sick Children (SickKids); University of Toronto; University of Toronto; Stockholm University; University of Otago; Queensland University of Technology (QUT); University of Alberta
摘要：To investigate the structure of the mycobacterial oxidative phosphorylation machinery, we prepared inverted membrane vesicles from Mycobacterium smegmatis, enriched for vesicles containing complexes of interest, and imaged the vesicles with electron cryomicroscopy. We show that this analysis allows determination of the structure of both mycobacterial ATP synthase and the supercomplex of respiratory complexes III and IV in their native membrane. The latter structure reveals that the enzyme mala...
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作者：Gao, Yirui; Barton, John P.
作者单位：University of California System; University of California Riverside; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh
摘要：Natural selection often acts on multiple traits simultaneously. For example, the virus HIV-1 faces pressure to evade host immunity while also preserving replicative fitness. While past work has studied selection during HIV-1 evolution, as in other examples where selection acts on multiple traits, it is challenging to quantitatively separate different contributions to fitness. This task is made more difficult because a single mutation can affect both immune escape and replication. Here, we deve...
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作者：Ravindran, Sandeep
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作者：Chandler, Talon; Guo, Min; Su, Yijun; Chen, Jiji; Wu, Yicong; Liu, Junyu; Agashe, Atharva; Fischer, Robert S.; Mehta, Shalin B.; Kumar, Abhishek; Baskin, Tobias I.; Jaumouille, Valentin; Liu, Huafeng; Swaminathan, Vinay; Nain, Amrinder S.; Oldenbourg, Rudolf; La Riviere, Patrick J.; Shroff, Hari
作者单位：Chan Zuckerberg Initiative (CZI); University of Chicago; Zhejiang University; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB); National Institutes of Health (NIH) - USA; Howard Hughes Medical Institute; Virginia Polytechnic Institute & State University; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI); Marine Biological Laboratory - Woods Hole; University of Massachusetts System; University of Massachusetts Amherst; Marine Biological Laboratory - Woods Hole; Simon Fraser University; Scania; Lund University; Scania; Lund University
摘要：Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations in biological samples, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the diffraction-limited three-dimensional distribution of the orientations and positions of ensembles of fluorescent dipoles that label biological structures. We share a set of visualization, histogram, an...
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作者：Li, Liangzi; Scott, W. Shem; Khristich, Alexandra N.; Armenia, Jillian F.; Mirkin, Sergei M.
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作者：Viegas, Jennifer
