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作者：Wan, Zhixin; Li, Cun; Zhou, Ying; Yu, Yifei; Chiu, Man Chun; Huang, Jingjing; Zhang, Shuxin; Zhu, Xiaoxin; Lan, Qiaoshuai; Deng, Yanlin; Xue, Wei; Jiang, Chengfan; Wu, Jiali; Zhao, Zijun; Cai, Jian-Piao; Huang, Lin; Zhang, Yong; Liu, Xiaojuan; Zhang, Zheng; Chu, Hin; Zhang, Linqi; Chen, Zhiwei; To, Kelvin Kai-Wang; Yuen, Kwok-Yung; Clevers, Hans; Zhou, Jie
作者单位：University of Hong Kong; Peking University; The Third People's Hospital of Shenzhen; University of Hong Kong; University of Hong Kong; Tsinghua University; Tsinghua University; Tsinghua University; Royal Netherlands Academy of Arts & Sciences; Hubrecht Institute (KNAW); Utrecht University; Utrecht University Medical Center; Roche Holding
摘要：The efficacy of VIR-7831, a class 3 anti-SARS-CoV-2 monoclonal antibody (mAb), was demonstrated repeatedly in clinical trials; yet, reduced neutralization against Omicron variants in cell-line-based neutralization assays led to its withdrawal from clinical use. We developed organoid-based neutralization assays to measure mAb potency. We found that most class 3 mAbs, especially those not blocking receptor-binding domain-ACE2 binding, including VIR-7831, were substantially underestimated in cell...
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作者：Gu, Feng; Naghavi, Mojgan H.
作者单位：Northwestern University; Feinberg School of Medicine
摘要：Intracellular multivesicular bodies (MVBs) act as sites of assembly and release of HIV type 1 (HIV-1) in macrophages and microglia. Recent work has shown that processing of amyloid precursor protein (APP) into a C-terminal fragment (CTF), termed C99, inhibits HIV-1 access to CD63+ MVBs and to counteract this, HIV-1 Group-specific antigen (Gag) increases C99 processing into toxic amyloids. However, the underlying reasons for this negative interplay between Gag and C99 remain unclear. Here, we s...
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作者：Kim, Haeun; Tian, Ya-Min; Ratcliffe, Peter J.; Keeley, Thomas P.
作者单位：University of Oxford; University of Oxford; Ludwig Institute for Cancer Research; Francis Crick Institute; University of Oxford
摘要：Selected proteins containing an N-terminal cysteine (Nt-Cys) are subjected to rapid, O2-dependent proteolysis via the Cys/Arg-branch of the N-degron pathway. Cysteine dioxygenation is catalyzed in mammalian cells by 2-aminoethanethiol dioxygenase (ADO), an enzyme that manifests extreme O2 sensitivity.The canonical substrates of this pathway in mammalia are the regulators of G-protein signaling 4, 5, and 16, as well as interleukin-32. In addition to operating as an O2-sensing mechanism, this pa...
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作者：Lee, Geonhui; Jo, Hye -Jin; Choi, Jihoon; Guzman, Maria Fonseca; Shan, Yu; Le, Han K. D.; Feijoo, Julian; Soland, Nathan; Clark, Douglas S.; Yang, Peidong
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory
摘要：The valorization of CO2 to chemicals beyond C1-2 products is receiving significant interest; however, the direct electrosynthesis of C-n molecules (n > 4) remains a challenge. Here, we present a two-step abiotic-biotic system for upgrading CO2 into the biopolymer, poly(3-hydroxybutyrate). In the electrolysis system, CO2 is converted into C-2 oxygenates using a Cu-Ag tandem electrocatalyst. The electrolysis process generates a liquid stream containing similar to 200 mM acetate in a bio-compatib...
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作者：Rojas, Margarita; Carrier, Rosalind Williams -; Chotewutmontri, Prakitchai; Belcher, Susan; Boyce, Emily; Barkan, Alice
作者单位：University of Illinois System; University of Illinois Urbana-Champaign; United States Department of Agriculture (USDA)
摘要：The D1 subunit of photosystem II (PSII) is subject to light- induced damage. In plants, D1 photodamage activates translation of chloroplast psbA mRNA encoding D1, providing D1 for PSII repair. Three D1 assembly factors have been implicated in the regulatory mechanism: HCF244 and RBD1 activate psbA translation, whereas HCF136 represses psbA translation in the dark. To clarify the regulatory circuit, we analyzed psbA ribosome occupancy in dark- adapted and illuminated rbd1 and rbd1;hcf136 double...
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作者：Xiao, Wanbo; Pan, Lu; Wang, Yanbin; Li, Jiaqi
作者单位：Peking University; Chinese Academy of Sciences; University of Science & Technology of China, CAS
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作者：Yuan, Sichen; Yang, Xicheng; Bremmer, Alexa M.; Wang, Yixin; Li, Sherry; Chen, Yu; You, Yawen; Hu, Quanyin
作者单位：University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison
摘要：Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive cancer with limited treatment options due to its desmoplastic and immunosuppressive tumor microenvironpoint blockade (ICB) has shown poor efficacy in PDAC, partly due to the desmoplastic stroma and low immunogenicity. Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 (Pin1) promotes both fibrosis and immune evasion, making it a compelling target PD-L1 blockade to enhance immunotherapy. This system uses Escherichia coli Nissle ...
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作者：Cai, Xun; Han, Zhaoyu; Li, Zi-Xiang; Kivelson, Steven A.; Yao, Hong
作者单位：Chinese Academy of Sciences; Institute of Physics, CAS; Stanford University; Tsinghua University
摘要：A quantum spin liquid (QSL) is an exotic insulating phase with emergent gauge fields and fractionalized excitations. However, the unambiguous demonstration of the existence of a QSL in a nonengineered microscopic model (or in any material) remains challenging. Here, using numerically exact sign-problem-free quantum Monte Carlo simulations, we show that a QSL arises in a nonengineered electron-phonon model. Specifically, we investigate the ground-state phase diagram of the bond Su-Schrieffer-He...
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作者：Huang, Aobo; Zhang, Jinming; Liu, Zhendong; Schoen, Vanessa; Verma, Deepanjali; Zheng, Haiyan; Pedmale, Ullas, V; Dong, Juan
作者单位：Rutgers University System; Rutgers University New Brunswick; Texas A&M University System; Texas A&M University College Station; Cold Spring Harbor Laboratory; Rutgers University System; Rutgers University New Brunswick; Rutgers University Biomedical & Health Sciences; Rutgers University System; Rutgers University New Brunswick; Rutgers University System; Rutgers University New Brunswick
摘要：The formation of a body axis is one of the fundamental steps in developmental patterning in multicellular organisms. Ectopic expression of the stomatal protein BASL (BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE) reveals a proximal-distal cell polarity field in the leaf and an apical-basal field in the hypocotyl and root of Arabidopsis. This provides a framework for uncovering molecular components of body-axis cell polarity in higher plants. In this study, we developed a proximity labeling-bas...
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作者：Ravindran, Sandeep
摘要：Chris Greene grew up with an early interest in science. During his undergraduate studies at the University of Nebraska in Lincoln, he realized he wanted to focus on theoretical atomic, molecular, and optical (AMO) physics. With help from influential mentors such as his PhD advisor Ugo Fano, Greene went on to have a long and distinguished career as a theorist working in AMO physics and chemical physics. Over his career, he helped develop theories of few-body systems, particularly in ultracold g...
