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作者：Petrovic, Ivana; Tatli, Meltem; Desai, Samit; Grahl, Anne; Ni, Dongchun; Stahlberg, Henning; Spang, Anne; Grzesiek, Stephan; Abiko, Layara Akemi
作者单位：University of Basel; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; University of Lausanne
摘要：Only two nonvisual arrestins recognize many hundreds of different, intracellularly phosphorylated G protein-coupled receptors (GPCRs). Due to the highly dynamic nature of GPCR center dot arrestin complexes, the critical determinants of GPCR-arrestin recognition have remained largely unclear. We show here that arrestin2 recruitment to the R1-adrenergic receptor (R1AR) can be induced by an arrestin-activating phosphopeptide that is not covalently linked to the receptor and that the recruitment i...
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作者：Yan, Xingchi; Yu, Polly Y.; Srinivasan, Arvind; Rehman, Sohaib Abdul; Sreenivas, Surabhi Kottigegollahalli; Conway, Jeremy B.; Prigozhin, Maxim B.
作者单位：Harvard University; Harvard University; Harvard University; University of Illinois System; University of Illinois Urbana-Champaign
摘要：Intermolecular interactions underlie all cellular functions, yet visualizing these interactions at the single-molecule level remains challenging. Single-molecule localization microscopy (SMLM) offers a potential solution. Given a nanoscale map of two putative interaction partners, it should be possible to assign molecules either to the class of coupled pairs or to the class of noncoupled bystanders. Here, we developed a probabilistic algorithm that allows accurate determination of both the abs...
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作者：Nguyen, Rita H.; Newton, Michelle; Kratofil, Rachel M.; Scott, Brittney N. V.; Castanheira, Fernanda; Kim, Jung- seok; Ginhoux, Florent; Jung, Steffen; Kubes, Paul
作者单位：University of Calgary; Weizmann Institute of Science; Chinese Academy of Sciences; Shanghai Jiao Tong University; Agency for Science Technology & Research (A*STAR); A*STAR - Singapore Immunology Network (SIgN); UNICANCER; Gustave Roussy
摘要：Concussions can cause debilitating symptoms despite no evidence of structural changes on diagnostic imaging. The cellular events occurring in the brain parenchyma following concussion, especially repetitive concussion, are not well elucidated. We developed a concussion model to induce a confined area of injury without causing frank hemorrhage. Using intravital microscopy, we observe activation of the vasculature that supported neutrophil rolling and platelet adhesion but no overt cellular recr...
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作者：Wang, Xiao; Tsien, Sophia J.; Jin, Min; Goldberg, Michael E.; Zhang, Mingsha; Qian, Ning
作者单位：Beijing Normal University; Columbia University; Columbia University; Columbia University; Columbia University; Columbia University; Columbia University
摘要：We perceive a stable, continuous world despite drastic changes of retinal images across saccades. However, while persistent objects in daily life appear stable across saccades, stimuli flashed around saccades can be grossly mislocalized. We address this puzzle with our recently proposed circuit model for perisaccadic receptive-field (RF) remapping in the lateral interparietal area (LIP) and frontal eye fields (FEF). The model uses center-excitation/surround-inhibition connections to store a re...
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作者：Lord, Kathryn A.; Larson, Greger; Allaby, Robin G.; Karlsson, Elinor K.
作者单位：University of Massachusetts System; University of Massachusetts Worcester; UMass Chan Medical School; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; University of Oxford; University of Warwick
摘要：The process of domestication is commonly perceived as a human achievement, and domestic species are typically assumed to be those under human control. Domestic species have emerged from a greater diversity of interactions than this perspective allows, and none of the many definitions proposed for domestication can readily, reliably, and consistently distinguish domestic and nondomestic populations. Here, we propose that the process of domestication should instead be defined solely as evolution...
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作者：Xu, Luo; Zeng, Hongtai; Lin, Ning; Yang, Yue; Guo, Qinglai; Poor, H. Vincent
作者单位：Princeton University; Princeton University; Tsinghua University; Hefei University of Technology; Hefei University of Technology; Princeton University
摘要：Distribution networks, with large-scale integration of distributed renewable resources, particularly rooftop solar photovoltaic systems, represent the most extensive yet vulnerable components of modern electric power systems during climate extremes such as hurricanes. However, existing day-ahead electricity dispatch approaches primarily focus on the transmission network and lack the capability to manage the spatiotemporal risks associated with the vast distribution networks, which can potentia...
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作者：Fang, Lisa Z.; Hirasawa, Michiru
作者单位：Washington University (WUSTL); Memorial University Newfoundland
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作者：Ma, Wei; Ma, Lai - Peng; Kong, Xiao; Yan, Han; Liu, Zhibo; Han, Tiannan; Zhu, Chao; Cheng, Hui - Ming; Liu, Zheng; Ding, Feng; Ren, Wencai
作者单位：Chinese Academy of Sciences; Institute of Metal Research, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Nanyang Technological University; Chinese Academy of Sciences; Shanghai Institute of Microsystem & Information Technology, CAS; Chinese Academy of Sciences; Shenzhen Institute of Advanced Technology, CAS; National University of Singapore; Institute for Functional Intelligent Materials (I-FIM); Suzhou Laboratory
摘要：State- of- the- art synthesis strategies of two- dimensional (2D) materials have been designed following the nucleation- dominant pattern for structure control. However, this classical methodology fails to achieve the precise layer- and stacking- resolved growth of wafer- scale few- layer 2D materials due to its intrinsically low energy resolution. Here, we present an intelligent self- correcting method for the high- resolution growth of uniform few- layer graphene. We demonstrate the layer- r...
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作者：Shriver, Robert K.; Pletcher, Elise; Biondi, Franco; Urza, Alexandra K.; Weisberg, Peter J.
作者单位：Nevada System of Higher Education (NSHE); University of Nevada Reno; United States Department of Agriculture (USDA); United States Forest Service
摘要：Recent increases in woody plant density in dryland ecosystems-or woody encroachment-around the world are often attributed to land-use changes such as increased livestock grazing and wildfire suppression or to global environmental trends (e.g., increasing atmospheric carbon dioxide). While such changes have undoubtedly impacted ecosystem structure and function, the evidence linking them to woody encroachment is mixed, and the underlying processes are not fully understood. To clarify the role of...
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作者：McDonald, Eli Fritz; Kim, Minsoo; Olson, John A.; Meiler, Jens; Plate, Lars
作者单位：Vanderbilt University; Vanderbilt University; Vanderbilt University; Vanderbilt University; Leipzig University; Vanderbilt University; Vanderbilt University
摘要：Cystic fibrosis (CF) is a lethal genetic disorder caused by variants in CF transmembrane conductance regulator (CFTR). Many variants are treatable with correctors, which enhance the folding and trafficking of CFTR. However, approximately 3% of persons with CF harbor poorly responsive variants. Here, we used affinity purification mass spectrometry proteomics to profile the protein homeostasis (proteostasis) changes of CFTR variants during correction to assess modulated interactions with protein...
