-
作者：Mehmood, Sultan; Naseer, Shaheen; Chen, Daniel L.
-
作者：Smith, Carter S.; Demattia, Elizabeth A.; Albright, Elizabeth; Bromberger, Abigail F.; Hayward, Olivia G.; Mackinson, India J.; Mantell, Sydney A.; Mcadoo, Brian G.; Mcafee, Dominic; Mccollum, Aurora; Paxton, Avery B.; Roderer, Anne; Stevenson, Kathryn; Vidra, Rebecca L.; Zhao, Zixin
作者单位：Duke University; University of Washington; University of Washington Seattle; Duke University; Duke University; University of Adelaide; National Oceanic Atmospheric Admin (NOAA) - USA; National Ocean Service, NOAA; National Centers for Coastal Ocean Science (NOOCS); National Oceanic Atmospheric Admin (NOAA) - USA; North Carolina State University
摘要：Ecosystem restoration has historically been viewed as an ecological endeavor, but restoration possesses significant, yet largely untapped, potential as a catalyst for personal and social transformation. We highlight the opportunity for restoration to enhance community resilience by increasing agency and collective action and countering the pervasive perception that we are powerless witnesses to environmental decline. In this perspective, we take a bright spots approach and highlight successful...
-
作者：Bai, Xuefei; Xu, Lingyi; Wang, Zhe; Zhuang, Xinlei; Ning, Jiangtao; Sun, Yanping; Wang, Haibin; Guo, Yugang; Xu, Yingchun; Guo, Jiangtao; Chen, Shuqing; Pan, Liqiang
作者单位：Zhejiang University; Zhejiang University
摘要：Recurrent missense mutations in the human epidermal growth factor receptor 2 (HER2) have been identified across various human cancers. Among these mutations, the active S310F mutation in the HER2 extracellular domain stands out as not only oncogenic but also confers resistance to pertuzumab, an antibody drug widely used in clinical cancer therapy, by impeding its binding. In this study, we have successfully employed computational- aided rational design to undertake directed evolution of pertuz...
-
作者：Chen, Peng; Hu, Jiang-Jian; Liu, Yuexin; Cao, Boxu; Song, Xue-Jun
作者单位：Southern University of Science & Technology
摘要：Ubiquitin- proteasomal degradation of K+/Cl- cotransporter 2 (KCC2) in the ventral posteromedial nucleus (VPM) has been demonstrated to serve as a common mechanism by which the brain emerges from anesthesia and regains consciousness. Ubiquitin- proteasomal degradation of KCC2 during anesthesia is driven by E3 ligase Fbxl4. However, the mechanism by which ubiquitinated KCC2 is targeted to the proteasome has not been elucidated. We report in cultured neuro-2a cells that the valosin- containing p...
-
作者：Ngo, Wayne; Peukes, Julia; Baldwin, Alisha; Xue, Zhiwei Wayne; Hwang, Sidney; Stickels, Robert R.; Lin, Zhi; Satpathy, Ansuman T.; Wells, James A.; Schekman, Randy; Nogales, Eva; Doudna, Jennifer A.; Bjorkman, Pamela
作者单位：University of California System; University of California Berkeley; University of California System; University of California San Francisco; The J David Gladstone Institutes; University of California System; University of California Berkeley; University of California System; University of California Berkeley; Stanford University; University of California System; University of California San Francisco; University of California System; University of California San Francisco; Howard Hughes Medical Institute; University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; University of California System; University of California Berkeley
摘要：The widespread application of genome editing to treat and cure disease requires the delivery of genome editors into the nucleus of target cells. Enveloped delivery vehicles (EDVs) are engineered virally derived particles capable of packaging of lentiviral genome encapsulation and replication proteins in EDVs has obscured the underlying delivery mechanism and precluded particle optimization. Here, we show that Cas9 RNP nuclear delivery is independent of the native lentiviral capsid structure. I...
-
作者：Pelletier, Julie M.; Chen, Min; Lin, Jer-Young; Le, Brandon; Kirkbride, Ryan C.; Hur, Jungim; Wang, Tina; Chang, Shu-Heng; Olson, Alexander; Nikolov, Lachezar; Goldberg, Robert B.; Harada, John J.
作者单位：University of California System; University of California Davis; University of California System; University of California Los Angeles; Academia Sinica - Taiwan; University of California System; University of California Riverside; University of Texas System; University of Texas Austin; National Chung Hsing University; Indiana University System; Indiana University Bloomington
摘要：Seeds are complex structures composed of three regions, embryo, endosperm, and seed coat, with each further divided into subregions that consist of tissues, cell layers, and cell types. Although the seed is well characterized anatomically, much less is known about the genetic circuitry that dictates its spatial complexity.To address this issue, we profiled mRNAs from anatomically distinct seed subregions at several developmental stages. Analyses of these profiles showed that all subregions exp...
-
作者：Vernie, Tatiana; Rich, Melanie; Pellen, Tifenn; Teyssier, Eve; Garrigues, Vincent; Chauderon, Lucie; Medioni, Laurena; van Beveren, Fabian; Libourel, Cyril; Keller, Jean; Girou, Camille; Lefort, Corinne; Le Ru, Aurelie; Martinez, Yves; Reinhardt, Didier; Kodama, Kyoichi; Shimazaki, Shota; Morel, Patrice; Kyozuka, Junko; Mbengue, Malick; Harrison, Maria; Vandenbussche, Michiel; Delaux, Pierre- Marc
作者单位：Universite Federale Toulouse Midi-Pyrenees (ComUE); Universite de Toulouse; Institut National Polytechnique de Toulouse; Centre National de la Recherche Scientifique (CNRS); Universite de Toulouse; Universite Toulouse III - Paul Sabatier; University of Fribourg; Tohoku University; Centre National de la Recherche Scientifique (CNRS); Ecole Normale Superieure de Lyon (ENS de LYON); Universite Claude Bernard Lyon 1; INRAE
摘要：Plants have colonized lands 450 million years ago. This terrestrialization was facilitated by developmental and functional innovations. Recent evo-devo approaches have demonstrated that one of these innovations was the mutualistic arbuscular mycorrhizal symbiosis (AMS). The genetic pathways that have been involved in the establishment and functioning of AMS since its evolution remain poorly described. Here, we found that intracellular colonization by AM fungi induces a transcriptional reporter...
-
作者：Carlson, Maryn O.; Andrews, Bryan L.; Simons, YuvalB.
作者单位：University of Chicago; University of Chicago; University of Chicago; University of Chicago; University of Chicago; University of Chicago
摘要：The phenotypic effect of a mutation may depend on the genetic background in which it occurs, a phenomenon referred to as epistasis. One source of epistasis in proteins is direct interactions between residues in close physical proximity to one another. However, epistasis may also occur in the absence of specific interactions between amino acids if the genotype-to-phenotype map is nonlinear. Disentangling the contributions of these two phenomena-specific and global epistasis-from noisy, high-thr...
-
作者：V. Vasylyev, Dmytro; Zhao, Peng; Schulman, Betsy R.; Waxman, Stephen G.
作者单位：Yale University; Yale University; US Department of Veterans Affairs; Veterans Health Administration (VHA); VA Connecticut Healthcare System
摘要：Voltage-gated sodium channel Nav1.8 plays a crucial role in regulating excitability of small dorsal root ganglion (DRG) neurons and is an emerging target for pain therapeutics. Using dynamic clamp, we systematically manipulated Nav1.8 conductance to assess its impact on action potential (AP) electrogenesis, rheobase, and repetitive firing in native rat DRG neurons and those expressing the gain-of-function Nav1.7L858H mutation which underlies inherited erythromelalgia, a human genetic pain diso...
-
作者：van Gestel, Jordi; Koo, Byoung-Mo; Sturmer, Vanessa S.; Garriga-Canut, Mireia; Wagner, Jonas; Zanon, Andrea; Gross, Carol A.
作者单位：University of California System; University of California San Francisco; European Molecular Biology Laboratory (EMBL); European Molecular Biology Laboratory (EMBL); European Molecular Biology Laboratory (EMBL); Ruprecht Karls University Heidelberg; Ruprecht Karls University Heidelberg
摘要：Single-cell eukaryotic predators in the soil are a primary cause of bacterial cell death. Yet, most functional genomic studies on soil bacteria have been performed without predation, thereby selecting for phenotypes impacting growth rather than survival and biasing our view on the ecological factors driving genomic evolution. Here, we study how predation by the ubiquitous amoebal predator Dictyostelium discoideum affects Bacillus subtilis' growth and survival using both a genome-scale mutant s...
