Proceedings of the National Academy of Sciences of the United States of America

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• A WRKY transcription factor confers broad-spectrum resistance to biotic stresses and yield stability in rice AI翻译
  作者：Liu, Daoming; He, Jun; Li, Qi; Zhang, Xiao; Wang, Yongsheng; Sun, Quanguang; Wang, Wenhui; Zhang, Menglong; Wang, Yunlong; Xu, Haosen; Fang, Liang; Jiang, Ling; Liu, Shijia; Chen, Liangming; Tian, Yunlu; Liu, Xi; Wang, Ruyi; Zhang, Zhengguang; Chern, Mawsheng; Dong, Xiaoou; Wang, Haiyang; Liu, Yuqiang; Ronald, Pamela C.; Wan, Jianmin
  作者单位：Nanjing Agricultural University; Chinese Academy of Agricultural Sciences; Institute of Plant Protection, CAAS; Nanjing Agricultural University; University of California System; University of California Davis; University of California System; University of California Davis; Chinese Academy of Agricultural Sciences; Institute of Crop Sciences, CAAS; United States Department of Energy (DOE); Joint BioEnergy Institute - JBEI
  摘要：Plants are subject to attack by diverse pests and pathogens. Few genes conferring broad-spectrum resistance to both insects and pathogens have been identified. Because of the growth-defense tradeoff, it is often challenging to balance biotic stress resistance and yield for crops. Here, we report that OsWRKY36 suppresses the resistance to insects and pathogens via transcriptional repression of Phenylalanine Ammonia Lyases (PALs), a key enzyme in phenylpropanoid pathway in rice. Knocking out OsW...
• Adeno-associated viruses for efficient gene expression in the axolotl nervous system AI翻译
  作者：Lust, Katharina; Tanaka, Elly M.
  作者单位：Austrian Academy of Sciences; Vienna Biocenter (VBC); Institute of Molecular Biotechnology (IMBA)
  摘要：Axolotls are amphibian models for studying nervous system evolution, development, and regeneration. Tools to visualize and manipulate cells of the axolotl nervous system with high-efficiency, spatial and temporal precision are therefore greatly required. Recombinant adeno-associated viruses (AAVs) are frequently used for in vivo gene transfer of the nervous system but virus-mediated gene delivery to the axolotl nervous system has not yet been described. Here, we demonstrate the use of AAVs for...
• EGFR-induced lncRNA TRIDENT promotes drug resistance in non-small cell lung cancer via phosphoTRIM28-mediated DNA damage repair AI翻译
  作者：Saxena, Tanvi; Quan, Anan; Chan, Erica; Kozlova, Nina; Matai, Latika; Lee, Jonathan D.; Rupaimoole, Rajesha; Beca, Francisco; Muranen, Taru; Slack, Frank J.
  作者单位：Harvard University; Harvard University Medical Affiliates; Beth Israel Deaconess Medical Center; Harvard Medical School; Harvard University; Harvard University Medical Affiliates; Beth Israel Deaconess Medical Center; Harvard Medical School; Bayer AG; Bayer Healthcare Pharmaceuticals; Merck & Company
  摘要：Long noncoding RNAs (lncRNAs) play numerous roles in cellular biology and alterations in lncRNA expression profiles have been implicated in a variety of cancers. Here, we identify and characterize a lncRNA, TRIM28 Interacting DNA damage repair Enhancing Noncoding Transcript (TRIDENT), whose expression is induced upon epithelial growth factor receptor (EGFR) activation, and which exerts pro-oncogenic functions in EGFR-driven non-small cell lung cancer. Knocking down TRIDENT leads to decreased t...
• Early evidence of avocado domestication from El Gigante Rockshelter, Honduras AI翻译
  作者：VanDerwarker, Amber M.; Thakar, Heather B.; Hirth, Kenneth; Domic, Alejandra I.; Harper, Thomas K.; George, Richard J.; Johnson, Emily S.; Newhall, Victoria; Scheffler, Timothy E.; McCool, Weston C.; Wann, Kevin; Gaut, Brandon S.; Kistler, Logan; Kennett, Douglas J.
  作者单位：University of California System; University of California Santa Barbara; Texas A&M University System; Texas A&M University College Station; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; Pennsylvania Commonwealth System of Higher Education (PCSHE); Pennsylvania State University; Pennsylvania State University - University Park; University of California System; University of California Los Angeles; Utah System of Higher Education; University of Utah; University of California System; University of California Irvine; Smithsonian Institution; Smithsonian National Museum of Natural History
  摘要：Molecular research suggests that avocados (Persea americana Mill.) were domesticated multiple times in the Americas. Seed exchange, hybridization, and cloning have played an essential role across their wild distribution from Mexico to South America to create the modern varieties of today. Archaeological sites with well- preserved and directly radiocarbon- dated botanical assemblages are rare, however, so we know very little about the complexities of the domestication process. Here, we define a...
• Structural variant and nucleosome occupancy dynamics postchemotherapy in a HER2+breast cancer organoid model AI翻译
  作者：Starostecka, Maja; Jeong, Hyobin; Hasenfeld, Patrick; Garagorri, Eva Benito -; Christiansen, Tania; Brasseur, Catherine Stober; Queiroz, Maise Gomes; Montero, Marta Garcia; Jechlinger, Martin; Korbel, Jan O.
  作者单位：Bristol-Myers Squibb; European Molecular Biology Laboratory (EMBL); Yonsei University; Helmholtz Association; German Cancer Research Center (DKFZ); European Molecular Biology Laboratory (EMBL)
  摘要：The most common chemotherapeutics induce DNA damage to eradicate cancer cells, yet defective DNA repair can propagate mutations, instigating therapy resistance and secondary malignancies. Structural variants (SVs), arising from copy- number- imbalanced and - balanced DNA rearrangements, are a major driver of tumor evolution, yet understudied posttherapy. Here, we adapted single- cell with nucleosome occupancy (NO) measurements obtained from the same single cell to enable simultaneous SV detect...
• Toward equitable major histocompatibility complex binding predictions AI翻译
  作者：Glynn, Eric; Ghersi, Dario; Singh, Mona
  作者单位：Princeton University; Princeton University; University of Nebraska System; University of Nebraska Omaha; Princeton University
  摘要：Deep learning tools that predict peptide binding by major histocompatibility complex (MHC) proteins play an essential role in developing personalized cancer immunotherapies and vaccines. In order to ensure equitable health outcomes from their application, MHC binding prediction methods must work well across the vast landscape of MHC alleles observed across human populations. Here, we show that there are alarming disparities across individuals in different racial and ethnic groups in how much b...
• Modeling energy requirements for oxygen production on AI翻译
  作者：Leger, Dorian; Ghaffari-Tabrizi, Fardin; Shaw, Matthew; Rasera, Joshua; Dickson, David; Valentin, Baptiste; Morlock, Anton; Thoresen, Freja; Cowley, Aidan
  作者单位：European Space Agency; European Astronaut Center; Commonwealth Scientific & Industrial Research Organisation (CSIRO); CSIRO Mineral Resources; Imperial College London; Colorado School of Mines; Helmholtz Association; Karlsruhe Institute of Technology
  摘要：Spacecraft using combustion engines require substantial amounts of oxygen for their propellant. The Moon could be a source of oxygen for rocket propellant, since the material composing the lunar surface can be processed to extract oxygen. However, little is known about overall energy requirements of the processes described in the literature for oxygen extraction from lunar regolith. This knowledge gap constrains the planning of lunar missions, since the scale of energy infrastructure required ...
• Adapting C4 photosynthesis to atmospheric change and increasing productivity by elevating Rubisco content in sorghum and sugarcane AI翻译
  作者：Salesse-Smith, Coralie E.; Adar, Noga; Kannan, Baskaran; Nguyen, Thaibinhduong; Wei, Wei; Guo, Ming; Ge, Zhengxiang; Altpeter, Fredy; Clemente, Tom E.; Long, Stephen P.
  作者单位：University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; State University System of Florida; University of Florida; State University System of Florida; University of Florida; University of Nebraska System; University of Nebraska Lincoln; University of Nebraska System; University of Nebraska Lincoln; University of Illinois System; University of Illinois Urbana-Champaign
  摘要：Meta- analyses and theory show that with rising atmospheric [CO2], Rubisco has become the greatest limitation to light- saturated leaf CO2 assimilation rates (A(sat)) in C-4 crops. So would transgenically increasing Rubisco increase A(sat) and result in increased productivity in the field? Here, we successfully overexpressed the Rubisco small subunit (RbcS) with Rubisco accumulation factor 1 (Raft) in both sorghum and sugarcane, resulting in significant increases in Rubisco content of 13 to 25...
• Spatial population dynamics of bacterial colonies with social antibiotic resistance AI翻译
  作者：Denk-Lobnig, Marlis K.; Wood, Kevin B.
  作者单位：University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
  摘要：Bacteria frequently inhabit surface- attached communities where rich social interac-tions can significantly alter their population- level behavior, including their response to antibiotics. Understanding these collective effects in spatially heterogeneous communi-ties is an ongoing challenge. Here, we investigated the spatial organization that emerges from antibiotic exposure in initially randomly distributed communities containing antibiotic- resistant and - sensitive strains of Enterococcus f...
• Inhibition of GABARAP or GABARAPL1 prevents aminoglycoside- induced hearing loss AI翻译
  作者：Li, Jinan; Oh, Seung-Il; Liu, Chang; Zhao, Bo
  作者单位：Indiana University System; Indiana University Bloomington
  摘要：Aminoglycosides (AGs) are highly potent, broad- spectrum antibiotics frequently used as first- line treatments for multiple life- threatening infections. Despite their severe oto-toxicity, causing irreversible hearing loss in millions of people annually, no preventive therapy has been approved. We previously reported that GABARAP and several other central autophagy proteins are essential for AG- induced hearing loss. This finding opens avenues for the rational design and development of inhibit...