Adapting C4 photosynthesis to atmospheric change and increasing productivity by elevating Rubisco content in sorghum and sugarcane

• 成果类型: Article
• 署名作者: Salesse-Smith, Coralie E.; Adar, Noga; Kannan, Baskaran; Nguyen, Thaibinhduong; Wei, Wei; Guo, Ming; Ge, Zhengxiang; Altpeter, Fredy; Clemente, Tom E.; Long, Stephen P.
• 署名单位: University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; State University System of Florida; University of Florida; State University System of Florida; University of Florida; University of Nebraska System; University of Nebraska Lincoln; University of Nebraska System; University of Nebraska Lincoln; University of Illinois System; University of Illinois Urbana-Champaign
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9921
• DOI: 10.1073/pnas.2419943122
• 发表日期: 2025-02-25
• 关键词: catalytic-properties; large-subunit; co2; c-4; maize; photorespiration; FUTURE; state; WILL

摘要

Meta- analyses and theory show that with rising atmospheric [CO2], Rubisco has become the greatest limitation to light- saturated leaf CO2 assimilation rates (A(sat)) in C-4 crops. So would transgenically increasing Rubisco increase A(sat) and result in increased productivity in the field? Here, we successfully overexpressed the Rubisco small subunit (RbcS) with Rubisco accumulation factor 1 (Raft) in both sorghum and sugarcane, resulting in significant increases in Rubisco content of 13 to 25% and up to 90% respectively. A(sat) increased 12 to 15% and Rubisco enzyme activity similar to 40% in three independent transgenic events of both species. Sorghum plants also showed increased speeds of photosynthetic induction and decreased bundle sheath leakiness. These improvements translated into average increases of 15.5% in biomass in field- grown sorghum and a 37 to 81% increase in greenhouse- grown sugarcane. This suggests a potential opportunity to achieve substantial increases in productivity of this key economically important clade of C-4 crops, future proofing their value under global atmospheric change.