2期

最新文章

• A chemogenetic approach for temporal and cell-specific activation of endogenous GPCRs in vivo AI翻译
  作者：Shingles, Gwendolyn; Pang, Qianqian; Weng, Jian; Singer, Ryan; Babar, Waleed; Shen, Jiaqi; Vazquez-Rivera, Luis; Chen, Yao; Li, Peng; Wang, Wenjing
  作者单位：Peking University; Shanghai Jiao Tong University; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; Washington University (WUSTL); Washington University (WUSTL); University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
  摘要：Cell-specific regulation of endogenous G protein-coupled receptors (GPCRs) is crucial for understanding their roles in physiological processes. We present chemogenetic tools using shield-1-dependent irreversible protein switches to regulate peptide agonist activity. To demonstrate this platform, we engineered chemogenetically regulated pituitary adenylate cyclase activating polypeptide (cPACAP), which exhibited >15-fold chemical-dependent regulation of endogenous receptor activity. In vivo app...
• Imputing unjustified bulk density values to soils with biochar addition biases soil carbon sequestration estimates AI翻译
  作者：Sun, Daquan
  作者单位：Czech Academy of Sciences; Biology Centre of the Czech Academy of Sciences
• Hyperglycemia promotes SIRT3-mediated deacetylation of SARM1 to exacerbate diabetic peripheral neuropathy in mice AI翻译
  作者：Chen, Chunyu; Zhu, Liliang; Li, Wenxi; Jiang, Yuefeng; Zhang, Zhe; Zhao, Yongjuan; Lin, Jinzhong; Yu, Wei; Cobb, Melanie
  作者单位：Fudan University; Peking University; The Chinese University of Hong Kong, Shenzhen
  摘要：Diabetic peripheral neuropathy (DPN), the most common complication of diabetes, lacks effective treatments and is characterized by early axonal degeneration mediated by sterile alpha and Toll/interleukin receptor motif-containing protein 1 (SARM1). Here, we identify a regulatory mechanism of SARM1's NAD+-cleaving activity via acetylation at lysine 641 (K641). In high-glucose conditions, SIRT3 deacetylates SARM1 at K641, enhancing its NAD+ cleavage activity and exacerbating axonal damage. In ty...
• Supersaturation-engineered wafer-scale growth of anisotropic 2D organic crystals for uniform polarimetric sensing AI翻译
  作者：Shen, Xianfeng; Wu, Xianshuo; Yao, Ximeng; Ma, Chunli; Sun, Lingjie; Wang, Zhaofeng; Gao, Pichao; Zeng, Dong; Deng, Wei; Jie, Jiansheng; Yang, Fangxu; Li, Rongjin; Hu, Wenping
  作者单位：Tianjin University; Tianjin University; Tianjin University; Soochow University - China
  摘要：Organic single crystals endowed with intrinsic anisotropy hold great promise for the realization of miniaturized, polarization-sensitive photodetectors. However, conventional dip-coating approaches struggle to produce wafer-scale single-crystalline films, as the supersaturation conditions optimized for nucleation invariably conflict with those required for crystal growth, leading to fragmented domains and compromised device performance. Here, we introduce an in situ seeded dip coating (SDC) st...
• Emergent neuronal mechanisms mediating covert attention in convolutional neural networks (vol. 122, e2411909122, 2025) AI翻译
  作者：Srivastava, Sudhanshu; Wang, William Yang; Eckstein, Miguel P.
• Molecular basis of the higher-order assembly of CatSper AI翻译
  作者：Xu, Qikui; Lin, Shiyi; Zhao, Qingqing; Ru, Yanfei; Kang, Hang; Zeng, Xuhui; Jiang, Min; Yan, Zhen; Wu, Jianping
  作者单位：Westlake University; Westlake University; Westlake Laboratory; Westlake University; Westlake University; Nantong University
  摘要：CatSper serves as the primary Ca2+ entry pathway in the principal piece of sperm flagellum and is crucial for sperm motility and fertility. Sperm lacking functional CatSper channels fail to undergo hyperactivation during fertilization, leading to complete male infertility. Along the longitudinal axis of the sperm flagellum, staggered CatSper complexes align in a hand-in-hand arrangement, forming a distinctive quadrilinear zigzag arrays known as CatSper nanodomains. However, the molecular detai...
• Origin of class B J-domain proteins involved in amyloid transactions AI翻译
  作者：Domanski, Przemyslaw; Stolarska, Milena; Kalinowska, Katarzyna; Purzycki, Dominik; Schilke, Brenda A.; Wyszkowski, Hubert; Pitek, Marcin; Szymanska, Aneta; Bury, Katarzyna; Czub, Jacek; Klosowska, Agnieszka A.; Craig, Elizabeth A.; Marszalek, Jaroslaw; Tomiczek, Bartlomiej
  作者单位：Fahrenheit Universities; University of Gdansk; Fahrenheit Universities; Gdansk University of Technology; University of Wisconsin System; University of Wisconsin Madison; Fahrenheit Universities; University of Gdansk
  摘要：J--domain protein (JDP) chaperones function widely in proteostasis. Notably, eukaryotic class B JDPs of the cytosol/nucleus prevent assembly or drive disassembly of amyloid aggregates known to cause neurodegenerative diseases, yet their evolutionary origin is not known. Members of the most ubiquitous class B subgroup, canonical B (BC) JDPs, lack the signature zinc finger domain (ZnF) of the more prevalent class A JDPs, while having other key features in common. Our phylogenetic analysis reveal...
• Engineered and decellularized human cartilage graft exhibits intrinsic immunosuppressive properties and full skeletal repair capacity AI翻译
  作者：Garcia, Alejandro Garcia; Prithiviraj, Sujeethkumar; Raina, Deepak Bushan; Schmidt, Tobias; Anton, Sara Gonzalez; Cajal, Laura Rabanal; Gil, David Hidalgo; Tagil, Magnus; Hyrenius-Wittsten, Axel; Dahlgren, Madelene W.; Kahn, Robin; Bourgine, Paul E.
  作者单位：Lund University; Lund University; Lund University; Lund University; Lund University; Lund University; Lund University
  摘要：Tissue engineering strategies predominantly consist of the autologous generation living substitutes capable of restoring damaged body parts. Persisting challenges with patient-specific approaches include inconsistent performance, high costs, and delayed graft availability. Toward developing a one-for-all solution, a more attractive paradigm lies in the exploitation of dedicated cell lines for the fabrication of human tissue grafts. Following decellularization, this new class of biomaterials re...
• Elevator mechanism dynamics in a sodium-coupled dicarboxylate transporter AI翻译
  作者：Kinz-Thompson, Colin D.; Lopez-Redondo, Maria Louisa; Mulligan, Christopher; Sauer, David B.; Marden, Jennifer J.; Song, Jinmei; Tajkhorshid, Emad; Hunt, John F.; Stokes, David L.; Mindell, Joseph A.; Wang, Da-Neng; Gonzalez Jr, Ruben L.
  作者单位：Columbia University; New York University; University of Kent; University of Illinois System; University of Illinois Urbana-Champaign; Columbia University; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS); Columbia University; Cornell University; Weill Cornell Medicine; NewYork-Presbyterian Hospital; Rutgers University System; Rutgers University Newark; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Instituto de Biomedicina de Valencia (IBV); University of Oxford
  摘要：VcINDY, the sodium-dependent dicarboxylate transporter from Vibrio cholerae, is responsible for C4-carboxylate uptake into cells. The molecular mechanism of how VcINDY physically moves substrates across the membrane, and does so in an energetically efficient manner, is unclear. Here, we use single-molecule fluorescence resonance energy transfer experiments to directly observe the individual mechanistic steps that VcINDY takes to translocate substrates across a lipid bilayer, and then test key ...
• Reframing the axon initial segment: Giant ankyrin-G as a modulator of excitability and plasticity in neurodevelopment AI翻译
  作者：Lopez, Angel Y.; Jenkins, Paul M.
  作者单位：University of Michigan System; University of Michigan; University of Michigan System; University of Michigan