2期

最新文章

• Optimizing mRNA delivery with targeted elastin-like polypeptide-based LENN formulations: Insights into the endocytosis mechanism AI翻译
  作者：Darji, Saloni; Qu, Feng; Henager, Marissa; Prasad, Ankita; Elzey, Bennett D.; Ferreira, Christina R.; Thompson, David H.
  作者单位：Purdue University System; Purdue University; Purdue University System; Purdue University; Purdue University System; Purdue University
  摘要：Gene delivery has emerged as a groundbreaking technique for altering gene expression, offering new possibilities in treating a vast array of diseases. We report a layer-by-layer elastin-like polypeptide nucleic acid nanoparticle (LENN) system for mRNA delivery as an attractive alternative to viral vectors and lipid nanoparticle (LNP) systems. This study focuses on determining the physical characteristics of LENN bearing mRNA cargo and assessing their biological performance in T24 bladder tumor...
• Biological causes and impacts of rugged tree landscapes in phylodynamic inference AI翻译
  作者：Gao, Jiansi; Brusselmans, Marius; Carvalho, Luiz M.; Suchard, Marc A.; Baele, Guy; Matsen, Frederick A.
  作者单位：Fred Hutchinson Cancer Center; KU Leuven; Getulio Vargas Foundation; University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; David Geffen School of Medicine at UCLA; University of California Los Angeles Medical Center; University of California System; University of California Los Angeles; David Geffen School of Medicine at UCLA; University of California Los Angeles Medical Center; Howard Hughes Medical Institute; University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle
  摘要：Phylodynamic analysis has been instrumental in elucidating epidemiological and evolutionary dynamics of pathogens. Bayesian phylodynamics integrates out phylogenetic uncertainty, which is typically substantial in phylodynamic datasets due to limited genetic diversity. Phylodynamic inference does not, however, scale with modern datasets, partly due to difficulties in traversing tree space. Here, we characterize tree space and landscape in phylodynamic inference and assess its impacts on analysi...
• GATA2 facilitates steroid receptor coactivator recruitment to the androgen receptor complex (vol 111, pg 18266, 2014) AI翻译
  作者：He, Bin; Lanz, Rainer B.; Fiskus, Warren; Geng, Chuandong; Yi, Ping; Hartig, Sean M.; Rajapakshe, Kimal; Shou, John; Wei, Liping; Shah, Shrijal S.; Foley, Christopher; Chew, Sue Anne; Eedunuri, Vijay K.; Bedoya, Diego J.; Feng, Qin; Minami, Takashi; Mitsiades, Constantine S.; Frolov, Anna; Weigel, Nancy L.; Hilsenbeck, Susan G.; Rosen, Daniel G.; Palzkill, Timothy; Ittmann, Michael M.; Song, Yongcheng; Coarfa, Cristian; O'Malley, Bert W.; Mitsiades, Nicholas
• Systematic identification of single transcription factor perturbations that drive cellular and tissue rejuvenation AI翻译
  作者：Sengstack, Janine; Zheng, Jiashun; Aghayev, Turan; Bieri, Gregor; Mobaraki, Michael; Lin, Jue; Deng, Changhui; Villeda, Saul A.; Li, Hao
  作者单位：University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco; University of California System; University of California San Francisco
  摘要：Cellular rejuvenation through transcriptional reprogramming is an exciting approach to counter aging. Using a fibroblast-based model of human cell aging and Perturb-seq screening, we developed a systematic approach to identify single transcription factor (TF) perturbations that promote rejuvenation without dedifferentiation. Overexpressing E2F3 or EZH2, and repressing STAT3 or ZFX, reversed cellular hallmarks of aging- increasing proliferation, proteostasis, and mitochondrial activity, while d...
• Hydroxo-bridged active site of flavodiiron NO reductase revealed by NRVS and DFT AI翻译
  作者：Folgosa, Filipe; Pelmenschikov, Vladimir; Caserta, Giorgio; Keck, Matthias; Lorent, Christian; Laun, Konstantin; Yoda, Yoshitaka; Gee, Leland B.; Kaupp, Martin; Tamasaku, Kenji; Birrell, James A.; Sergueev, Ilya; Limberg, Christian; Teixeira, Miguel; Lauterbach, Lars
  作者单位：Universidade Nova de Lisboa; Technical University of Berlin; Humboldt University of Berlin; Japan Synchrotron Radiation Research Institute; United States Department of Energy (DOE); Stanford University; SLAC National Accelerator Laboratory; University of Essex; Helmholtz Association; Deutsches Elektronen-Synchrotron (DESY); RWTH Aachen University
  摘要：The use of oxygen and nitrate as terminal electron acceptors provides organisms with a huge amount of available energy but necessitates methods to detoxify reactive intermediates. The mechanisms of NO and O2 detoxification in many organisms involve flavodiiron proteins (FDPs). Although the proteinaceous ligands that coordinate the diiron active site of these enzymes are well established, its exact coordination environment remains under debate due to conflicting interpretations of crystallograp...
• Supporting Information for Li et al., p53 deficiency induces MTHFD2 transcription to promote cell proliferation and restrain DNA damage (vol 118, e2019822118,2021) AI翻译
  作者：Li, Gen; Wu, Jun; Li, Le; Jiang, Peng
• Unintended consequences of well-intended interventions AI翻译
  作者：Perc, Matjaz
  作者单位：University of Maribor; Kyung Hee University; Korea University
• Phase-transition-like behaviors of sequence-selective dynamic bonds AI翻译
  作者：Dai, Xiaobin; Wang, Yuming; Wei, Wenjie; Jiao, Zheng; Chen, Wenlong; Cheng, Linghe; Yan, Li-Tang
  作者单位：Tsinghua University
  摘要：Sequence-selective dynamic bonds (SSDBs) are ubiquitous in nature and man-made systems as diverse as DNA, proteins, and synthetic sequence-defined oligomers. The specific and dynamic nature of this class of bonds enables encoded linkers to program the self-assembly of a broad range of building blocks. However, the possible collective behavior emerging from multiple SSDBs in programmable self-assembly remains elusive, due to experimental challenges. Here, we analyze the thermodynamic properties...
• A pothole-filling strategy for selective targeting of rCUG-repeats associated with myotonic dystrophy type 1 AI翻译
  作者：Perera, J. Dinithi R.; Thadke, Shivaji A.; Thrikawala, Savani W.; Dhami, Isha; Hridya, V. M.; Mukherjee, Arnab; Paul, Ananya; Wilson, W. David; Tan, Keith W. R.; Chan, Nicholas Z. W.; Phan, Anh Tuan; Ly, Danith H.
  作者单位：Carnegie Mellon University; Carnegie Mellon University; Indian Institute of Science Education & Research (IISER) Pune; University System of Georgia; Georgia State University; Nanyang Technological University
  摘要：We present an alternative approach to conventional small-molecule and antisense strategies for selectively targeting expanded CUG-RNA repeats associated with Myotonic Dystrophy type 1. Our alternatively designed nucleic acid ligands uniquely integrate advantageous features from both existing methods: They are compact (only three units in length), structurally resembling small molecules, yet recognize RNA targets through directional hydrogen-bonding similar to antisense oligonucleotides. Notabl...
• Hypersensitivity of chitin degradation to initial species densities due to monomer diffusion AI翻译
  作者：Pontrelli, Sammy; Guessous, Ghita; Trouillon, Julian; Krishna, Aswin; Hwa, Terence; Sauer, Uwe
  作者单位：Swiss Federal Institutes of Technology Domain; ETH Zurich; KU Leuven; Flanders Institute for Biotechnology (VIB); University of California System; University of California San Diego
  摘要：Resource competition strongly shapes microbial community dynamics and functionality. In polysaccharide-degrading communities, primary degraders release hydrolytic enzymes, whereas exploiters consume released products without producing enzyme themselves. We investigate the competitive strategies employed by marine chitin degraders and N-acetylglucosamine (GlcNAc) exploiters, revealing various mechanisms that impact community viability and growth dynamics. In addition to direct competition strat...