A chemogenetic approach for temporal and cell-specific activation of endogenous GPCRs in vivo

• 成果类型: Article
• 署名作者: Shingles, Gwendolyn; Pang, Qianqian; Weng, Jian; Singer, Ryan; Babar, Waleed; Shen, Jiaqi; Vazquez-Rivera, Luis; Chen, Yao; Li, Peng; Wang, Wenjing
• 署名单位: Peking University; Shanghai Jiao Tong University; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; Washington University (WUSTL); Washington University (WUSTL); University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2501228123
• 发表日期: 2026-01-13
• 页码: e2501228123
• 关键词: POLYPEPTIDE PACAP; messenger-rna; PEPTIDE PACAP; receptor; nucleus; stress; brain; interrogation; proteins; disorder

摘要

Cell-specific regulation of endogenous G protein-coupled receptors (GPCRs) is crucial for understanding their roles in physiological processes. We present chemogenetic tools using shield-1-dependent irreversible protein switches to regulate peptide agonist activity. To demonstrate this platform, we engineered chemogenetically regulated pituitary adenylate cyclase activating polypeptide (cPACAP), which exhibited >15-fold chemical-dependent regulation of endogenous receptor activity. In vivo application of cPACAP allowed neuronal activation via the endogenous receptor for PACAP, engaging neural circuits that control respiratory and feeding behaviors. By integrating cPACAP with transgenic mice, we selectively activated endogenous PACAP receptor signaling in hypocretin-expressing neurons of the lateral hypothalamic area (LHA), revealing its role in regulating sighing, a stress-related physiological output. We further extended this design to chemogenetically regulate the parathyroid hormone receptor and corticotropin-releasing factor peptide receptor activity. Using a common small molecule, these chemogenetic tools enable temporally regulated peptidergic activation of endogenous GPCRs in targeted cell populations, facilitating the study of their function.