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作者：Illibauer, Jennifer; Clodi- Seitz, Tamara; Zoufay, Alexander; Aberle, Judith H.; Weninger, Wolfgang J.; Foedinger, Manuela; Elsayad, Kareem
作者单位：Medical University of Vienna; Medical University of Vienna; Medical University of Vienna
摘要：Blood plasma viscosity (PV) is an established biomarker for numerous diseases. Measurement of the shear PV using conventional rheological techniques is, however, time consuming and requires significant plasma volumes. Here, we show that Brillouin light scattering (BLS) and angle- resolved spectroscopy measurements of the longitudinal PV from microliter- sized plasma volumes can serve as a proxy for the shear PV measured using conventional viscometers. This is not trivial given the distinct fre...
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作者：Yu, Guoyu; Corn, Paul G.; Mak, Celia Sze Ling; Liang, Xin; Zhang, Miao; Troncoso, Patricia; Song, Jian H.; Lin, Song - Chang; Song, Xingzhi; Liu, Jingjing; Zhang, Jianhua; Logothetis, Christopher J.; Melancon, Marites P.; Panaretakis, Theocharis; Wang, Guocan; Lin, Sue - Hwa
作者单位：University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center; University of Texas System; UTMD Anderson Cancer Center
摘要：Immune checkpoint therapy has limited efficacy for patients with bone- metastatic castration- resistant prostate cancer (bmCRPC). To improve immunotherapy for bmCRPC, we aimed to identify the mechanism of bmCRPC- induced changes in the immune microenvironment. Among bmCRPC patients, higher levels of a 32- gene M2- like macrophage signature in bone metastasis samples correlated with shorter overall survival. Immunohistochemistry showed that CD206- positive (CD206+) macrophages were enriched in ...
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作者：Croswell, Ken
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作者：Gaete, Pablo S.; Kumar, Deepak; Fernandez, Cynthia I.; Capuccino, Juan M. Valdez; Bhatt, Aashish; Jiang, Wenjuan; Lin, Yi - Chun; Liu, Yu; Harris, Andrew L.; Luo, Yun L.; Contreras, Jorge E.
作者单位：University of California System; University of California Davis; Western University of Health Sciences; Rutgers University System; Rutgers University New Brunswick; Rutgers University Biomedical & Health Sciences; Rutgers University Newark
摘要：Connexin hemichannels were identified as the first members of the eukaryotic large- pore channel family that mediate permeation of both atomic ions and small molecules between the intracellular and extracellular environments. The conventional view is that their pore is a large passive conduit through which both ions and molecules diffuse in a similar manner. In stark contrast to this notion, we demonstrate that the permeation of ions and of molecules in connexin hemichannels can be uncoupled a...
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作者：Hart, James L.; Pan, Haining; Siddique, Saif; Schnitzer, Noah; Mallayya, Krishnanand; Xu, Shiyu; Kourkoutis, Lena F.; Kim, Eun-ah; Cha, Judy J.
作者单位：Cornell University; Cornell University; Cornell University; Cornell University; Ewha Womans University
摘要：We study the coupled charge density wave (CDW) and insulator-to-metal transitions in the 2D quantum material 1T-TaS2. By applying in situ cryogenic 4D scanning transmission electron microscopy with in situ electrical resistance measurements, we directly visualize the CDW transition and establish that the transition is mediated by basal dislocations (stacking solitons). We find that dislocations can both nucleate and pin the transition and locally alter the transition temperature T-c by nearly ...
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作者：Liu, Xingyu; Zhang, Ying; Wen, Zhihui; Hao, Yan; Banks, Charles A. S.; Cesare, Joseph; Bhattacharya, Saikat; Arvindekar, Shreyas; Lange, Jeffrey J.; Xie, Yixuan; Garcia, Benjamin A.; Slaughter, Brian D.; Unruh, Jay R.; Viswanath, Shruthi; Florens, Laurence; Workman, Jerry L.; Washburn, Michael P.
作者单位：Stowers Institute for Medical Research; University of Kansas; University of Kansas Medical Center; Tata Institute of Fundamental Research (TIFR); National Centre for Biological Sciences (NCBS); Washington University (WUSTL); University of Texas System; University of Texas Southwestern Medical Center; University of Kansas; University of Kansas Medical Center
摘要：Serial capture affinity purification (SCAP) is a powerful method to isolate a specific protein complex. When combined with cross- linking mass spectrometry and computational approaches, one can build an integrated structural model of the isolated complex. Here, we applied SCAP to dissect a subpopulation of WDR76 in complex with SPIN1, a histone reader that recognizes trimethylated histone H3 lysine4 (H3K4me3). In contrast to a previous SCAP analysis of the SPIN1:SPINDOC complex, histones and t...
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作者：Boesen, Thias Oberg; Charbon, Godefroid; Fu, Haochen; Jensen, Cara; Sandler, Michael; Jun, Suckjoon; Lobner-Olesen, Anders
作者单位：University of Copenhagen; University of California System; University of California San Diego
摘要：Investigating a long-standing conceptual question in bacterial physiology, we examine why DnaA, the bacterial master replication initiator protein, exists in both ATP and ADP forms, despite only the ATP form being essential for initiation. We engineered the O4 Escherichia coli strain, devoid of all known external elements facilitating the DnaA-ATP/ADP conversion and found that these cells display nearly wild-type behaviors under nonoverlapping replication cycles. However, during rapid growth w...
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作者：Hohm, Ian; O'Shea, Brian A.; Schaller, Mark
作者单位：University of British Columbia; University of Nottingham; Aarhus University
摘要：Moral values guide consequential attitudes and actions. Here, we report evidence of seasonal variation in Americans' endorsement of some-but not all-moral values. Studies 1 and 2 examined a decade of data from the United States (total N = 232,975) and produced consistent evidence of a biannual seasonal cycle in values pertaining to loyalty, authority, and purity (binding moral values)-with strongest endorsement in spring and autumn and weakest endorsement in summer and winter-but not in values...
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作者：Liu, Fanghua; Ye, Fangyuan; Yang, Yunwen; Kang, Zhiwei; Liu, Yang; Chen, Wei; Wang, Saige; Kou, Hongru; Kang, Le; Sun, Jianghua
作者单位：Hebei University; Chinese Academy of Sciences; Institute of Zoology, CAS; Chinese Academy of Agricultural Sciences; Institute of Plant Protection, CAAS
摘要：Insects and their gut bacteria form a tight and beneficial relationship, especially in utilization of host nutrients. The red turpentine beetle (RTB), a destructive and invasive pine pest, employs mutualistic microbes to facilitate its invasion success. However, the molecular mechanism underlying the utilization of nutrients remains unknown. In this study, we found that gut bacteria are crucial for the utilization of D- glucose, a main carbon source for RTB development. Downstream assays revea...
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作者：Chiang, Huai - Chin; Qi, Leilei; Mitra, Payal; Huang, Yimeng; Hu, Yanfen; Li, Rong
作者单位：George Washington University; George Washington University
摘要：Deleterious accumulation of R- loops, a DNA-RNA hybrid structure, contributes to genome instability. They are associated with BRCA1 mutation- related breast cancer, an estrogen receptor alpha negative (ER alpha-) tumor type originating from luminal progenitor cells. However, a presumed causality of R- loops in tumorigenesis has not been established in vivo. Here, we overexpress mouse Rnaseh1 (Rh1-OE) in vivo to remove accumulated R- loops in Brca1- deficient mouse mammary epithelium (BKO). R- ...
