33期

最新文章

• Role of RNA structural plasticity in modulating HIV-1 genome packaging and translation AI翻译
  作者：Yasin, Saif; Lesko, Sydney L.; Kharytonchyk, Siarhei; Brown, Joshua D.; Chaudry, Issac; Geleta, Samuel A.; Tadzong, Ndeh F.; Zheng, Mei Y.; Patel, Heer B.; Kengni, Gabriel; Neubert, Emma; Quiambao, Jeanelle Mae C.; Becker, Ghazal; Ghinger, Frances Grace; Thapa, Sreeyasha; Williams, A'Lyssa; Radov, Michelle H.; Boehlert, Kellie X.; Hollmann, Nele M.; Singh, Karndeep; Bruce, James W.; Marchant, Jan; Sherer, Nathan M.; Telesnitsky, Alice; Summers, Michael F.
  作者单位：University System of Maryland; University of Maryland Baltimore County; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Michigan System; University of Michigan; University System of Maryland; University of Maryland Baltimore County; Howard Hughes Medical Institute; University System of Maryland; University of Maryland Baltimore County
  摘要：HIV-1 transcript function is controlled in part by twinned transcriptional start site usage, where 5 ' capped RNAs beginning with a single guanosine (1G) are preferentially packaged into progeny virions as genomic RNA (gRNA) whereas those beginning with three sequential guanosines (3G) are retained in cells as mRNAs. In 3G transcripts, one of the additional guanosines base pairs with a cytosine located within a conserved 5 ' polyA element, resulting in formation of an extended 5 ' polyA struct...
• Real- time single- molecule observation of incipient collagen fibrillogenesis and remodeling AI翻译
  作者：Roth, Jonathan; Hoop, Cody; Williams, Jonathan K.; Nanda, Vikas; Baum, Jean
  作者单位：Rutgers University System; Rutgers University New Brunswick; Rutgers University System; Rutgers University New Brunswick; Rutgers University Biomedical & Health Sciences; Rutgers University System; Rutgers University New Brunswick; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); Bristol-Myers Squibb
  摘要：The hierarchic assembly of fibrillar collagen into an extensive and ordered supramolecular protein fibril is critical for extracellular matrix function and tissue mechanics. Despite decades of study, we still know very little about the complex process of fibrillogenesis, particularly at the earliest stages where observation of rapidly forming, nanoscale intermediates challenges the spatial and temporal resolution of most existing microscan observe details of the first few minutes of collagen f...
• Fast and scalable ensemble learning method for versatile polygenic risk prediction AI翻译
  作者：Chen, Tony; Zhang, Haoyu; Mazumder, Rahul; Lin, Xihong
  作者单位：Harvard University; Harvard T.H. Chan School of Public Health; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); NIH National Cancer Institute- Division of Cancer Epidemiology & Genetics; Massachusetts Institute of Technology (MIT); Harvard University
  摘要：Polygenic risk scores (PRS) enhance population risk stratification and advance personalized medicine, but existing methods face several limitations, encompassing issues related to computational burden, predictive accuracy, and adaptability to a wide range of genetic architectures. To address these issues, we propose Aggregated L0Learn using Summary- level data (ALL- Sum), a fast and scalable ensemble learning method for computing PRS using summary statistics from genome-wide association studie...
• Mitochondrial genome copy number variation across tissues in mice and humans AI翻译
  作者：Rath, Sneha P.; Gupta, Rahul; Todres, Ellen; Wang, Hong; Jourdain, Alexis A.; Ardlie, Kristin G.; Calvo, Sarah E.; Mootha, Vamsi K.
  作者单位：Howard Hughes Medical Institute; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard Medical School; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; University of Lausanne
  摘要：The mammalian mitochondrial genome (mtDNA) is multicopy and its copy number (mtCN) varies widely across tissues, in development and in disease. Here, we systematically catalog this variation by assaying mtCN in 52 human tissues across 952 donors (10,499 samples from the Genotype- Tissue Expression project) and 20 murine tissues using qPCR, capturing 50- and 200- fold variation, respectively. We also estimate per cell mtCN across 173 human cell lines from the Cancer Cell Line Encyclopedia using...
• Single-particle rotational microrheology enables pathological staging of macrophage foaming and antiatherosclerotic studies AI翻译
  作者：Shang, Jinhui; Ma, Yuan; Liu, Xixuan; Sun, Shijie; Pang, Xiayun; Zhou, Rui; Huan, Shuangyan; He, Yan; Xiong, Bin; Zhang, Xiao-Bing
  作者单位：Hunan University; Shaanxi University of Chinese Medicine; Tsinghua University
  摘要：The formation of macrophage-derived foam cells has been recognized as the pathological hallmark of atherosclerotic diseases. However, the pathological evolution dynamics and underlying regulatory mechanisms remain largely unknown. Herein, we introduce a single-particle rotational microrheology method for pathological staging of macrophage foaming and antiatherosclerotic explorations by probing the dynamic changes of lysosomal viscous feature over the pathological evolution progression. The pri...
• A DNA condensation code for linker histones AI翻译
  作者：Watson, Matthew; Sabirova, Dilyara; Hardy, Megan C.; Pan, Yuming; Carpentier, David C. J.; Yates, Henry; Wright, Charlotte J.; Chan, W. H.; Destan, Ebru; Stott, Katherine
  作者单位：University of Cambridge; University of Cambridge
  摘要：Linker histones play an essential role in chromatin packaging by facilitating compaction of the 11-nm fiber of nucleosomal beads on a string. The result is a heterogeneous condensed state with local properties that range from dynamic, irregular, and liquid- like to stable and regular structures (the 30-nm fiber), which in turn impact chromatin- dependent activities at a fundamental level. The properties of the condensed state depend on the type of linker histone, particularly on the highly dis...
• Plant, insect, and fungi fossils under the center of Greenland's ice sheet are evidence of ice- free times AI翻译
  作者：Bierman, Paul R.; Mastro, Halley M.; Peteet, Dorothy M.; Corbett, Lee B.; Steig, Eric J.; Halsted, Chris T.; Caffee, Marc M.; Hidy, Alan J.; Balco, Greg; Bennike, Ole; Rock, Barry
  作者单位：University of Vermont; National Aeronautics & Space Administration (NASA); NASA Goddard Space Flight Center; Goddard Institute for Space Studies; Columbia University; University of Washington; University of Washington Seattle; Williams College; Purdue University System; Purdue University; United States Department of Energy (DOE); Lawrence Livermore National Laboratory; Geological Survey Of Denmark & Greenland; University System Of New Hampshire; University of New Hampshire
  摘要：The persistence and size of the Greenland Ice Sheet (GrIS) through the Pleistocene is uncertain. This is important because reconstructing changes in the GrIS determines its contribution to sea level rise during prior warm climate periods and informs future projections. To understand better the history of Greenland's ice, we analyzed glacial till collected in 1993 from below 3 km of ice at Summit, Greenland. The till contains plant fragments, wood, insect parts, fungi, and cosmogenic nuclides s...
• General theory for localizing the where and when of entropy production meets single-molecule experiments AI翻译
  作者：Deguenther, Julius; van der Meer, Jann; Seifert, Udo
  作者单位：University of Stuttgart
  摘要：The laws of thermodynamics apply to biophysical systems on the nanoscale as described by the framework of stochastic thermodynamics. This theory provides universal, exact relations for quantities like work, which have been verified in experiments where a fully resolved description allows direct access to such quantities. Complementary studies consider partially hidden, coarse-grained descriptions, in which the mean entropy production typically is not directly accessible but can be bounded in t...
• Leveraging a large language model to predict protein phase transition: A physical, multiscale, and interpretable approach AI翻译
  作者：Frank, Mor; Ni, Pengyu; Jensen, Matthew; Gerstein, Mark B.
  作者单位：Yale University; Yale University; Yale University; Yale University
  摘要：Protein phase transitions (PPTs) from the soluble state to a dense liquid phase (forming droplets via liquid-liquid phase separation) or to solid aggregates (such as amyloids) play key roles in pathological processes associated with age-related diseases such as Alzheimer's disease. Several computational frameworks are capable of separately predicting the formation of droplets or amyloid aggregates based on protein sequences, yet none have tackled the prediction of both within a unified framewo...
• Land conflicts from overlapping claims in Brazil's rural environmental registry AI翻译
  作者：Furumo, Paul R.; Yu, Jevan; Hogan, J. Aaron; de Carvalho, Luis M. Tavares; Brito, Brenda; Lambin, Eric F.
  作者单位：Stanford University; Stanford University; Massachusetts Institute of Technology (MIT); United States Department of Agriculture (USDA); United States Forest Service; Universidade Federal de Lavras
  摘要：Satellite- based land use monitoring and farm- level traceability offer opportunities for targeted zero- deforestation interventions on private lands. Brazil's Rural Environmental Registry (Cadastro Ambiental Rural, or CAR), a land cadaster based on self- declaration of property boundaries, was created to monitor compliance with national forest laws. It has become an important enabling measure for sustainable supply chain initiatives like the Amazon Soy Moratorium. However, CAR enrollment is i...