2024

最新文章

• Point mutations at specific sites of the nsp12-nsp8 interface dramatically affect the RNA polymerization activity of SARS- CoV-2 AI翻译
  作者：Ferrer-Orta, Cristina; Vazquez-Monteagudo, Sergi; Ferrero, Diego S.; Martinez-Gonzalez, Brenda; Perales, Celia; Domingo, Esteban; Verdaguer, Nuria
  作者单位：Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Instituto de Biologia Molecular de Barcelona (IBMB); Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Centro Nacional de Biotecnologia (CNB); Autonomous University of Madrid; Autonomous University of Madrid; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Centro de Biologia Molecular Severo Ochoa (CBM)
  摘要：In a recent characterization of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-- CoV- 2)- 2) variability present in 30 diagnostic samples from patients of the first COVID- 19- 19 pandemic wave, 41 amino acid substitutions were documented in the RNA-- dependent RNA polymerase (RdRp) nsp12. Eight substitutions were selected in this work to determine whether they had an impact on the RdRp activity of the SARS- CoV- 2- CoV- 2 nsp12-nsp8-nsp7 replication complex. Three of these substitut...
• Palmitoylation at a conserved cysteine residue facilitates gasdermin D- mediated pyroptosis and cytokine release AI翻译
  作者：Liu, Zhonghua; Li, Sai; Wang, Chuanping; Vidmar, Kaylynn J.; Bracey, Syrena; Li, Ling; Willard, Belinda; Miyagi, Masaru; Lan, Tong; Dickinson, Bryan C.; Osme, Abdullah; Pizarro, Theresa T.; Xiao, Tsan Sam
  作者单位：University System of Ohio; Case Western Reserve University; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Cleveland Clinic Foundation; University System of Ohio; Case Western Reserve University; University of Chicago; University of Alabama System; University of Alabama Birmingham
  摘要：Gasdermin D (GSDMD)-- mediated pyroptotic cell death drives inflammatory cytokine release and downstream immune responses upon inflammasome activation, which play important roles in host defense and inflammatory disorders. Upon activation by pro- teases, the GSDMD N-- terminal domain (NTD) undergoes oligomerization and membrane translocation in the presence of lipids to assemble pores. Despite intensive studies, the molecular events underlying the transition of GSDMD from an autoinhibited solu...
• Control of G protein-coupled receptor function via membrane- interacting intrinsically disordered C- terminal domains AI翻译
  作者：Mancinelli, Chiara D.; Marx, Dagan C.; Gonzalez -Hernandez, Alberto J.; Huynh, Kevin; Mancinelli, Lucia; Arefin, Anisul; Khelashvilli, George; Levitz, Joshua; Eliezer, David
  作者单位：Cornell University; Weill Cornell Medicine; Cornell University; Weill Cornell Medicine; Cornell University; Weill Cornell Medicine; Cornell University; Weill Cornell Medicine
  摘要：G protein-coupled receptors (GPCRs) control intracellular signaling cascades via agonist-- dependent coupling to intracellular transducers including heterotrimeric G proteins, GPCR kinases (GRKs), and arrestins. In addition to their critical interactions with the transmembrane core of active GPCRs, all three classes of transducers have also been reported to interact with receptor C-- terminal domains (CTDs). An underexplored aspect of GPCR CTDs is their possible role as lipid sensors given the...
• The gender agency gap in fiction writing (1850 to 2010) AI翻译
  作者：Stuhler, Oscar
  作者单位：Northwestern University; Max Planck Society
  摘要：Works of fiction play a crucial role in the production of cultural stereotypes. Concerning gender, a widely held presumption is that many such works ascribe agency to men and passivity to women. However, large-- scale diachronic analyses of this notion have been lacking. This paper provides an assessment of agency attributions in 87,531 fiction works written between 1850 and 2010. It introduces a syntax- based approach for extracting networks of character interactions. Agency is then formalize...
• Electrifying HCOOH synthesis from CO2 building blocks over Cu-Bi nanorod arrays AI翻译
  作者：Zhang, Guiru; Tan, Bing; Mok, Dong Hyeon; Liu, Huiya; Ni, Baoxin; Zhao, Gui; Ye, Ke; Huo, Shengjuan; Miao, Xiaohe; Liang, Zheng; Liu, Xi; Chen, Liwei; Zhang, Zemin; Cai, Wen Bin; Back, Seoin; Jiang, Kun
  作者单位：Shanghai Jiao Tong University; Lanzhou University; Sogang University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai University; Westlake University; Shanghai Jiao Tong University; Ningxia University; Fudan University
  摘要：Precise electrochemical synthesis of commodity chemicals and fuels from CO2 2 building blocks provides a promising route to close the anthropogenic carbon cycle, in which renewable but intermittent electricity could be stored within the greenhouse gas molecules. Here, we report state- of- the- art CO2- 2- to- HCOOH- HCOOH valorization performance over a multiscale optimized Cu-Bi cathodic architecture, delivering a formate Faradaic efficiency exceeding 95% within an aqueous electrolyzer, a C- ...
• Unraveling the mesoscale organization induced by network-driven processes AI翻译
  作者：Barzon, Giacomo; Artime, Oriol; Suweis, Samir; De Domenico, Manlio
  作者单位：University of Padua; Fondazione Bruno Kessler; University of Barcelona; University of Barcelona; Universitat de les Illes Balears; University of Padua; Istituto Nazionale di Fisica Nucleare (INFN); University of Padua
  摘要：Complex systems are characterized by emergent patterns created by the nontrivial interplay between dynamical processes and the networks of interactions on which these processes unfold. Topological or dynamical descriptors alone are not enough to fully embrace this interplay in all its complexity, and many times one has to resort to dynamics-specific approaches that limit a comprehension of general principles. To address this challenge, we employ a metric-that we name Jacobian distance- which c...
• Carbonate uranium isotopes record global expansion of marine anoxia during the Toarcian Oceanic Anoxic Event AI翻译
  作者：Remirez, Mariano N.; Gilleaudeau, Geoffrey J.; Gan, Tian; Kipp, Michael A.; Tissot, Francois L. H.; Kaufman, Alan J.; Parente, Mariano
  作者单位：George Mason University; University System of Maryland; University of Maryland College Park; University System of Maryland; University of Maryland College Park; Duke University; California Institute of Technology; University of Naples Federico II
  摘要：The Toarcian Oceanic Anoxic Event (T-OAE; similar to 183 Mya) was a globally significant carbon-cycle perturbation linked to widespread deposition of organic-rich sediments, massive volcanic CO2 release, marine faunal extinction, sea-level rise, a crisis in carbonate production related to ocean acidification, and elevated seawater temperatures. Despite recognition of the T-OAE as a potential analog for future ocean deoxygenation, current knowledge on the severity of global ocean anoxia is limi...
• Trichomonas vaginalis extracellular vesicles up- regulate and directly transfer adherence factors promoting host cell colonization AI翻译
  作者：Kochanowsky, Joshua A.; Mira, Portia M.; Elikaee, Samira; Muratore, Katherine; Rai, Anand Kumar; Riestra, Angelica M.; Johnson, Patricia J.
  作者单位：University of California System; University of California Los Angeles; California State University System; San Diego State University
  摘要：Trichomonas vaginalis , a common sexually transmitted parasite that colonizes the human urogenital tract, secretes extracellular vesicles (TvEVs) that are taken up by human cells and are speculated to be taken up by parasites as well. While the crosstalk between TvEVs and human cells has led to insight into host:parasite interactions, roles for TvEVs in infection have largely been one- sided, with little known about the effect of TvEV uptake by T. vaginalis . Approximately 11% of infections ar...
• A call for open science in forensics AI翻译
  作者：Albright, Thomas D.; Scurich, Nicholas
  作者单位：Salk Institute; University of California System; University of California Irvine; University of California System; University of California Irvine
  摘要：The modern canon of open science consists of five schools of thought that justify unfettered access to the fruits of scientific research: i) public engagement, ii) democratic right of access, iii) efficiency of knowledge gain, iv) shared technology, and v) better assessment of impact. Here, we introduce a sixth school: due process. Due process under the law includes a right to discovery by a defendant of potentially exculpatory evidence held by the prosecution. When such evidence is scientific...
• Bispecific antibodies targeting two glycoproteins on SFTSV exhibit synergistic neutralization and protection in a mouse model AI翻译
  作者：Chang, Zhen; Gao, Dan; Liao, Liying; Sun, Junqing; Zhang, Gen; Zhang, Xue; Wang, Feiran; Li, Chunrui; Oladejo, Babayemi Olawale; Li, Shihua; Chai, Yan; Hu, Yongfei; Lu, Xuancheng; Xiao, Haixia; Qi, Jianxun; Chen, Zhihai; Gao, Feng; Wu, Yan
  作者单位：Capital Medical University; Chinese Academy of Sciences; Institute of Microbiology, CAS; Shanxi Agricultural University; University of Macau; China Agricultural University; Chinese Center for Disease Control & Prevention; Chinese Academy of Sciences; Tianjin Institute of Industrial Biotechnology, CAS; Capital Medical University
  摘要：Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with a high fatality rate of up to 30% caused by SFTS virus (SFTSV). However, no specific vaccine or antiviral therapy has been approved for clinical use. To develop an effective treatment, we isolated a panel of human monoclonal antibodies (mAbs). SF5 and SF83 are two neutralizing mAbs that recognize two viral glycoproteins (Gn and Gc), respectively. We found that their epitopes are closely located, and we th...