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作者：Yuan, Yuan; Al Bulushi, Tahani; Sastry, Anand V.; Sancar, Cigdem; Szubin, Richard; Golden, Susan S.; Palsson, Bernhard O.
作者单位：University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego; University of California System; University of California San Diego; Novo Nordisk Foundation; Technical University of Denmark
摘要：Synechococcus elongatus is an important cyanobacterium that serves as a versatile and robust model for studying circadian biology and photosynthetic metabolism. Its transcriptional regulatory network (TRN) is of fundamental interest, as it orchestrates the cell's adaptation to the environment, including its response to sunlight. Despite the previous characterization of constituent parts of the S. elongatus TRN, a comprehensive layout of its topology remains to be established. Here, we decompos...
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作者：Gualdani, Roberta; Barbeau, Solene; Yuan, Jun - Hui; Jacobs, Deborah S.; Gailly, Philippe; Dib-Hajj, Sulayman D.; Waxman, Stephen G.
作者单位：Universite Catholique Louvain; Yale University; Yale University; US Department of Veterans Affairs; Veterans Health Administration (VHA); VA Connecticut Healthcare System; Harvard University; Harvard University Medical Affiliates; Massachusetts Eye & Ear Infirmary
摘要：The factors that contribute to pain after nerve injury remain incompletely understood. Laser- assisted in situ keratomileusis (LASIK) and photorefractive keratectomy (PRK) are common surgical techniques to correct refractive errors. After LASIK or PRK, a subset of patients suffers intense and persistent pain, of unknown origin, described by patients as feeling like shards of glass in their eye. Here, we evaluated a TRPV1 variant, p.V527M, found in a 49- y- old woman who developed corneal pain ...
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作者：Huang, Baolian; Yin, Te; Fu, Shuilian; Liu, Lina; Yang, Chen; Zhou, Lulu; Liu, Xing; Zhuang, Hongqin; Cao, Zhiting; Hua, Zichun
作者单位：China Pharmaceutical University; Nanjing University
摘要：Oral delivery of proteins faces challenges due to the harsh conditions of the gastrointestinal (GI) tract, including gastric acid and intestinal enzyme degradation. Permeation enhancers are limited in their ability to deliver proteins with high molecular weight and can potentially cause toxicity by opening tight junctions. To overcome these challenges, we propose the use of montmorillonite (MMT) as an adjuvant that possesses both inflammation- oriented abilities and the ability to regulate gut...
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作者：Ferguson, Michael A.; Asp, Erik W.; Kletenik, Isaiah; Tranel, Daniel; Boes, Aaron D.; Nelson, Jenae M.; Schaper, Frederic L. W. V. J.; Siddiqi, Shan; Turner, Joseph I.; Anderson, J. Seth; Nielsen, Jared A.; Bateman, James R.; Grafman, Jordan; Fox, Michael D.
作者单位：Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard Medical School; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard Medical School; University of Iowa; Mitchell Hamline School of Law; Mitchell Hamline School of Law; University of Iowa; University of Iowa; University of Iowa; Baylor University; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Harvard Medical School; Boston University; Brigham Young University; Wake Forest University; Northwestern University; Feinberg School of Medicine; Northwestern University; Feinberg School of Medicine; Northwestern University; Feinberg School of Medicine; Shirley Ryan AbilityLab
摘要：Religious fundamentalism, characterized by rigid adherence to a set of beliefs putatively revealing inerrant truths, is ubiquitous across cultures and has a global impact on society. Understanding the psychological and neurobiological processes producing religious fundamentalism may inform a variety of scientific, sociological, and cultural questions. Research indicates that brain damage can alter religious fundamentalism. However, the precise brain regions involved with these changes remain u...
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作者：Kazazian, Karnig; Abdalmalak, Androu; Novi, Sergio L.; Norton, Loretta; Ardakani, Reza Moulavi -; Kolisnyk, Matthew; Gofton, Teneille E.; Mesquita, Rickson C.; Owen, Adrian M.; Debicki, Derek B.
作者单位：Western University (University of Western Ontario); Western University (University of Western Ontario); Western University (University of Western Ontario); Western University (University of Western Ontario); University of Birmingham; Universidade Estadual de Campinas; Western University (University of Western Ontario)
摘要：Recent advancements in functional neuroimaging have demonstrated that some unresponsive patients in the intensive care unit retain a level of consciousness that is inconsistent with their behavioral diagnosis of awareness. Functional near- infrared spectroscopy (fNIRS) is a portable optical neuroimaging method that can be used to measure neural activity with good temporal and spatial resolution. However, the reliability of fNIRS for detecting the neural correlates of consciousness remains to b...
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作者：Lake, Cathleen M.; Gardner, Jennifer; Briggs, Salam; Yu, Zulin; McKown, Grace; Hawley, R. Scott
作者单位：Stowers Institute for Medical Research; University of Kansas; University of Kansas Medical Center
摘要：Meiosis is a form of cell division that is essential to sexually reproducing organisms and is therefore highly regulated. Each event of meiosis must occur at the correct developmental stage to ensure that chromosomes are segregated properly during both meiotic divisions. One unique meiosis- specific structure that is tightly regulated in terms of timing of assembly and disassembly is the synaptonemal complex (SC). While the mechanism(s) for assembly and disassembly of the SC are poorly underst...
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作者：Morland, Fay; Ewen, John G.; Santure, Anna W.; Brekke, Patricia; Hemmings, Nicola
作者单位：University of Sheffield; Zoological Society of London; University of Otago; University of Auckland
摘要：Hatching failure affects up to 77% of eggs laid by threatened bird species, yet the true prevalence and drivers of egg fertilization failure versus embryo mortality as underlying mechanisms of hatching failure are unknown. Here, using ten years of data comprising 4,371 eggs laid by a population of a threatened bird, the hihi (Notiomystis cincta), we investigate the relative importance of infertility and embryo death as drivers of hatching failure and explore population- level factors associate...
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作者：Findley, Michael G.; Ghosn, Faten; Lowe, Sara J.
作者单位：University of Texas System; University of Texas Austin; University of Essex; University of Texas System; University of Texas Austin
摘要：Ethics standards reference the need for special consideration of vulnerable populations, such as pregnant women, incarcerated individuals, and minors. The concept of vulnerability is poorly conceptualized in the medical sciences where it originated, and its application to the social sciences is even more challenging. Social science researchers may unwittingly fail to appreciate preexisting vulnerabilities and indeed may be responsible for inducing new research-related vulnerability. In this pa...
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作者：Ren, Wenlin; Fu, Chonglei; Zhang, Yu; Ju, Xiaohui; Jiang, Xi; Song, Jingwei; Gong, Mingli; Li, Zhuoyang; Fan, Wenchun; Yao, Jun; Ding, Qiang
作者单位：Tsinghua University; Tsinghua University; Shanxi Medical University; Shanxi Medical University; Zhejiang University
摘要：The ZIKA virus (ZIKV) evades the host immune response by degrading STAT2 through its NS5 protein, thereby inhibiting type I interferon (IFN)- mediated antiviral immunity. However, the molecular mechanism underlying this process has remained elusive. In this study, we performed a genome-wide CRISPR/Cas9 screen, revealing that ZSWIM8 as the substrate receptor of Cullin3-RING E3 ligase is required for NS5- mediated STAT2 degradation. Genetic depletion of ZSWIM8 and CUL3 substantially impeded NS5-...
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作者：Chechik, Maria; Greive, Sandra J.; Antson, Alfred A.; Jenkins, Huw T.
作者单位：University of York - UK; University of York - UK
摘要：DNA recognition is critical for assembly of double-stranded DNA viruses, particularly for the initiation of packaging the viral genome into the capsid. The key component that recognizes viral DNA is the small terminase protein. Despite prior studies, the molecular mechanism for DNA recognition remained elusive. Here, we address this question by identifying the minimal site in the bacteriophage HK97 genome specifically recognized by the small terminase and determining the structure of this comp...
