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作者：Lu, Yonggang; Ikawa, Masahito; Tang, Shaogeng
作者单位：University of Osaka; University of Osaka; University of Tokyo; University of Osaka; University of Osaka; Yale University
摘要：Sperm IZUMO1 binds to egg JUNO, and this interaction is essential for mammalian fertilization. Isolated from a female mouse immunized with syngeneic sperm, the antisperm antibody OBF13 recognizes IZUMO1 and inhibits murine fertilization. How OBF13 interferes with sperm-egg interactions was unknown. Here, we present the X-ray crystal structure of IZUMO1 in complex with OBF13. OBF13 binds to the apex of the four-helix domain of IZUMO1, distant from the JUNO-binding site. Our crystal structure of...
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作者：Xie, Boyang; Xiong, Tianyi; Guo, Guangguo; Pan, Cong; Ma, Wenjie; Yu, Ping
作者单位：Chinese Academy of Sciences; Institute of Chemistry, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS
摘要：The fluidic memristor has attracted growing attention as a promising candidate for neuromorphic computing and brain-computer interfaces. However, a fluidic memristor with ion selectivity as that of natural ion channels remains a key challenge. Herein, inspired by the structure of natural biomembranes, we developed an ion-shuttling memristor (ISM) by utilizing organic solvents and artificial carriers to emulate ion channels embedded in biomembranes, which exhibited both neuromorphic functions a...
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作者：Dvoriantchikova, Galina; Moulin, Chloe; Fleishaker, Michelle; Almeida, Vania; Pelaez, Daniel; Lam, Byron L.; Ivanov, Dmitry
作者单位：Bascom Palmer Eye Institute; University of Miami; University of Miami; University of Miami
摘要：The retina is responsible for converting light into electrical signals that, when transmitted to the brain, create the sensation of vision. The mammalian retina is epigenetically unique since the differentiation of retinal progenitor cells (RPCs) into retinal cells is accompanied by a decrease in DNA methylation in the promoters of many genes important for retinal development and function. However, the pathway responsible for DNA demethylation and its role in retinal development and function w...
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作者：Chadda, Ankita; Nguyen, Binh; Lohman, Timothy M.; Galburt, Eric A.
作者单位：Washington University (WUSTL)
摘要：UvrD- family helicases are superfamily 1A motor proteins that function during DNA replication, recombination, repair, and transcription. UvrD family monomers translocate along single- stranded (ss) DNA but need to be activated by dimerization to unwind DNA in the absence of force or accessory factors. However, prior structural studies have only revealed monomeric complexes. Here, we report the first structures of a dimeric UvrD- family helicase, Mycobacterium tuberculosis UvrD1, both free and ...
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作者：Wang, Jimin
作者单位：Yale University
摘要：Photosynthesis converts solar energy to chemical energy by splitting water molecules and carbon dioxide to produce oxygen and carbohydrates with an efficiency that engineers working on solar energy device can only dream of. Photosystem II (PSII) is the enzyme that catalyzes the light-driven oxidation of water that occurs during photosynthesis. This oxygen-producing reaction occurs in the Mn4Ca cluster found inside the enzyme's oxygen-evolving center (OEC). Even though the structure and mechani...
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作者：Basu, Arkaprabha; Krug, Tommy; du Pont, Benjamin; Huang, Qiaoling; Sun, Sijie; Adam, Stephen A.; Goldman, Robert D.; Weitz, David A.
作者单位：Harvard University; Xiamen University; Northwestern University; Feinberg School of Medicine; Harvard University; Harvard University
摘要：The cytoskeleton is composed of F- actin, microtubules, and intermediate filaments (IFs). Vimentin is one of the most ubiquitous and well- studied IFs. It is involved in many activities including wound healing, tissue fibrosis, and cancer metastasis, all of which require rapid vimentin IF assembly. In this paper, we report that vimentin forms liquid condensates which appear to enable rapid filament growth. Given the transient nature of these droplets, we focus on properties of vimentin- Y117L,...
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作者：Li, Zhu-Qin; Lei, Qun-Li; Ma, Yu-Qiang
作者单位：Collaborative Innovation Center of Advanced Microstructures (CICAM); Nanjing University; Nanjing University; Collaborative Innovation Center of Advanced Microstructures (CICAM); Hefei National Laboratory
摘要：Cells not only can be motile by crawling but are also capable of nonmotility active motions like periodic contraction or pulsation. In this work, based on a Voronoi cell model, we show how this nonmotility activity affects the structure, dynamic, and density fluctuations of cellular monolayers. Our model shows that random cell pulsation fluidizes solid epithelial tissues into a hyperuniform fluid state, while pulsation synchronization inhibits the fluidity and causes a reverse solidification. ...
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作者：Gruber, Christoph Heinrich; Noor, Elad; Buffing, Marieke Francisca; Sauer, Uwe
作者单位：Swiss Federal Institutes of Technology Domain; ETH Zurich; Weizmann Institute of Science
摘要：Recent physical binding screens suggest that protein-metabolite interactions are more extensive than previously recognized. To elucidate the functional relevance of these interactions, we developed a mass spectrometry-based screening method for higher throughput in vitro enzyme assays. By systematically quantifying the effects of 79 metabolites on the activity of 20 central Escherichia coli enzymes, we not only assess functional relevance but also gauge the depth of the current understanding o...
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作者：Huseby, Douglas L.; Cao, Sha; Zamaratski, Edouard; Sooriyaarachchi, Sanjeewani; Ahmad, Shabbir; Bergfors, Terese; Krasnova, Laura; Pelss, Juris; Ikaunieks, Martins; Loza, Einars; Katkevics, Martins; Bobileva, Olga; Cirule, Helena; Gukalova, Baiba; Grinberga, Solveiga; Backlund, Maria; Simoff, Ivailo Simof; Leber, Anna T.; Berruga-Fernandez, Talia; Antonov, Dmitry; Konda, Vivekananda R.; Lindstrom, Stefan; Olanders, Gustav; Brandt, Peter; Baranczewski, Pawel; Lundberg, Carina Vingsbo; Liepinsh, Edgars; Suna, Edgars; Jones, T. Alwyn; Mowbray, Sherry L.; Hughes, Diarmaid; Karlen, Anders
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作者：Ye, Hongnan
作者单位：China Medical University
