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作者：Straub, Verena M.; Barti, Benjamin; Tandar, Sebastian T.; Stevens, A. Floor; van Egmond, Noelle; van der Wel, Tom; Zhu, Na; Rueegger, Joel; van der Horst, Cas; Heitman, Laura H.; Li, Yulong; Stella, Nephi; van Hasselt, J. G. Coen; Katona, Istvan; van der Stelt, Mario
作者单位：Leiden University - Excl LUMC; Leiden University; Indiana University System; Indiana University Bloomington; Leiden University - Excl LUMC; Leiden University; Leiden University; Leiden University - Excl LUMC; Peking University; Peking University; University of Washington; University of Washington Seattle; HUN-REN; HUN-REN Institute of Experimental Medicine
摘要：While it is known that endocannabinoids (eCB) modulate multiple neuronal functions, the molecular mechanism governing their release and transport remains elusive. Here, we propose an on- demand release model, wherein the formation of microvesicles, a specific group of extracellular vesicles (EVs) containing the eCB, 2- arachidonoylglycerol (2- AG), is an important step. A coculture model system that combines a reporter cell line and neuronal cells revealed that neurons release EVs containing 2...
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作者：Huang, Han-Wen; Zeng, Yi-Fang; Shivatare, Vidya S.; Tseng, Tzu-Hao; Wong, Chi-Huey
作者单位：Scripps Research Institute
摘要：Glycosylation of humanized antibody at Fc- Asn297 significantly affects the Fc- mediated killing of target cells through effector functions, especially antibody- dependent cellular cytotoxicity (ADCC), antibody- dependent cell- mediated phagocytosis (ADCP), and antibody- dependent vaccinal effect (ADVE). Previous studies showed that therapeutic immunoglobulin G (IgG) antibodies with alpha 2,6- sialyl complex type (SCT) glycan attached to Fc- Asn297 exhibited optimal binding to the Fc receptors...
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作者：Battersby, Stephen
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作者：Yang, Darian T.; Chong, Lillian T.; Gronenborn, Angela M.
作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Carnegie Mellon University; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh
摘要：The HIV-1 capsid protein (CA) assembles into a conical shell during viral maturation, encasing and protecting the viral RNA genome. The C-terminal domain (CTD) the two-domain capsid protein dimerizes, and this dimer connects individual chains in the mature capsid lattice. Previous NMR studies have shown that different dimer arrangements can be formed by isolated capsid protein chains and in assembled capsid lattices; however, the dynamics and functional relevance of these alternate dimers are ...
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作者：Haddad, Hala K.; Mercado-Reyes, Jonathan I.; Mustafa, E. Roman; D'Souza, Shane P.; Chung, C. Sean; Nestor, Ramses R. M.; Olinski, Lauren E.; Damonte, Valentina Martinez; Saskin, Joshua; Vemaraju, Shruti; Raingo, Jesica; Kauer, Julie A.; Lang, Richard A.; Oancea, Elena
作者单位：Brown University; Cincinnati Children's Hospital Medical Center; Cincinnati Children's Hospital Medical Center; Stanford University; University System of Ohio; University of Cincinnati
摘要：Mammalian opsin 3 (OPN3) is a member of the opsin family of G- protein- coupled receptors with ambiguous light sensitivity. OPN3 was first identified in the brain (and named encephalopsin) and subsequently found to be expressed in other tissues. In adipocytes, OPN3 is necessary for light responses that modulate lipolysis and glucose uptake, while OPN3 in human skin melanocytes regulates pigmentation in a light- independent manner. Despite its initial discovery in the brain, OPN3 functional mec...
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作者：Liu, Benquan; Yasunaga, Jun-ichirou; Liang, Yi; Zhou, Ruoning; Yang, Sikai; Yuan, Xiaoyi; Liu, Jie; Zuo, Xiaorui; Miura, Michi; Higuchi, Yusuke; Matsumoto, Takashi; Toyoda, Kosuke; Matsuoka, Masao; Ma, Guangyong
作者单位：China Pharmaceutical University; Kumamoto University; Kyoto University
摘要：Adult T cell leukemia (ATL) is a highly aggressive T cell malignancy characterized by human T cell leukemia virus type 1 (HTLV-1) infection. ATL has a very poor prognosis and lacks satisfactory treatments; therefore, it is critical to identify potential targets in ATL cells in order to develop effective targeted therapeutics. Here, we report the identification of two oncogenes, AK4 and RHOC, as target genes of miR- 455- 3p, a tumor- suppressive microRNA in ATL patients. Importantly, AK4 and RH...
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作者：Priego-Cubero, Santiago; Knoch, Eva; Wang, Zhidan; Alseekh, Saleh; Braun, Karl-Heinz; Chapman, Philipp; Fernie, Alisdair R.; Liu, Chang; Becker, Claude
作者单位：University of Munich; University Hohenheim; Max Planck Society
摘要：Biosynthetic gene clusters (BGCs) are sets of often heterologous genes that are genetically and functionally linked. Among eukaryotes, BGCs are most common in plants and fungi and ensure the coexpression of the different enzymes coordinating the biosynthesis of specialized metabolites. Here, we report the identification of a withanolide BGC in Physalis grisea (ground- cherry), a member of the nightshade family (Solanaceae). A combination of transcriptomic, epigenomic, and metabolic analyses re...
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作者：Zecca, Marco A.; Greer, Heather F.; Muller, Karin H.; Duer, Melinda J.
作者单位：University of Cambridge; University of Cambridge
摘要：Bone calcification is essential for vertebrate life. The mechanism by which mineral ions are transported into collagen fibrils to induce intrafibrillar mineral formation requires a calcium binding biopolymer that also has highly selective binding to the collagen fibril hole zones where intrafibrillar calcification begins, over other bone extracellular matrix components. Poly(ADP- ribose) (PAR) has been shown to be a candidate biopolymer for this process and we show here that PAR has high affin...
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作者：Brewer, Kathryn R.; Vanoye, Carlos G.; Huang, Hui; Moster, Katherine R. Clowes; Desai, Reshma R.; Hayes, James B.; Burnette, Dylan T.; George, Alfred L.; Sanders, Charles R.
作者单位：Vanderbilt University; Vanderbilt University; Northwestern University; Feinberg School of Medicine; Vanderbilt University; Vanderbilt University
摘要：Loss- of- function (LOF) pathogenic variants in KCNQ1 encoding a cardiac potassium channel predispose to sudden cardiac death in type 1 congenital long QT syndrome (LQT1). To determine the spectrum of molecular mechanisms responsible for this life- threatening condition, we used an integrative approach to determine the biophysical, functional, and trafficking properties of 61 KCNQ1 variants distributed throughout all domains of the channel. Impaired trafficking to the plasma membrane was the m...
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作者：Chen, Jie; Zhu, Peiwen; Jin, Sihui; Zhang, Zhaokun; Jiang, Simei; Li, Sheng; Liu, Suning; Peng, Qionglin; Pan, Yufeng
作者单位：Southeast University - China; South China Normal University; South China Normal University; South China Normal University; Nantong University
摘要：Newborns, typically asexual, undergo a process of sexual transition to reach sexual maturity, but the regulatory mechanism underlying this transition is not clear. Here, we studied how female sexual behavior is modulated during sexual transition by hormones and neuromodulators in Drosophila. We found that neuropeptide Leucokinin (LK) inhibits female receptivity specifically during a sexual transition period in immature females, but not in younger or mature females. Moreover, the steroid hormon...
