Poly(ADP- ribose) binding sites on collagen I fibrils for nucleating intrafibrillar bone mineral

• 成果类型: Article
• 署名作者: Zecca, Marco A.; Greer, Heather F.; Muller, Karin H.; Duer, Melinda J.
• 署名单位: University of Cambridge; University of Cambridge
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13849
• DOI: 10.1073/pnas.2414849122
• 发表日期: 2025-02-20
• 关键词: identification; RECOGNITION; domains; aplf

摘要

Bone calcification is essential for vertebrate life. The mechanism by which mineral ions are transported into collagen fibrils to induce intrafibrillar mineral formation requires a calcium binding biopolymer that also has highly selective binding to the collagen fibril hole zones where intrafibrillar calcification begins, over other bone extracellular matrix components. Poly(ADP- ribose) (PAR) has been shown to be a candidate biopolymer for this process and we show here that PAR has high affinity, highly conserved binding sites in the collagen type I C- terminal telopeptides. The identification of these PAR-collagen binding sites gives insights into the chemical mechanisms underlying bone calcification and possible mechanisms behind pathologies where there is dysfunctional bone calcification.