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作者：Mattingly, Henry H.
作者单位：Simons Foundation; Flatiron Institute
摘要：Bacterial motility in spatially structured environments impacts a variety of natural and engineering processes. Constructing models to predict, control, and design bacterial motility for these processes remains challenging because bacteria and active swimmers have complex interactions with surfaces and because the precise environment geometry is unknown. Here, we present a method for deriving bacterial diffusion coefficients in disordered media in terms of cell and environmental parameters. Th...
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作者：Zhu, Tian-Chen; He, Zhang-Ping; Li, Shu-Ting; Zheng, Lin; Zheng, Xue-Yi; Lan, Xia-Lu; Qu, Chun-Hua; Nie, Run-Cong; Gu, Chao; Huang, Li-Ning; Cai, Xiao-Xia; Xiang, Zhi-Cheng; Xie, Dan; Cai, Mu-Yan
作者单位：State Key Lab Oncology South China; Sun Yat Sen University; State Key Lab Oncology South China; Sun Yat Sen University; Guangzhou Medical University; Fujian Medical University; Fujian Medical University; Sun Yat Sen University; State Key Lab Oncology South China; Nanjing Medical University
摘要：Poly-ADP-ribose polymerase (PARP) inhibitors are vital therapeutic agents that exploit synthetic lethality, particularly effective in tumors with homologous recombination (HR) defects. However, broadening their clinical utility remains a significant challenge. In this study, we conducted a high-throughput kinase inhibitor screen to identify potential targets exhibiting synthetical lethality with PARP inhibitors. Our results show that thousand and one amino acid protein kinase 1 (TAOK1) plays a...
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作者：Liu, Kang; Wu, Lina; Ma, Yuanyuan; Chen, Desheng; Liu, Rujia; Zhang, Xiaobo; Jiang, Dechen; Pan, Rongrong
作者单位：Nanjing University; Nanjing Normal University; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS
摘要：Simultaneous profiling of multiple molecules trafficking at a single organelle and the surrounding cytosol within a living cell is crucial for elucidating their functions, necessitating advanced techniques that provide high spatial-temporal resolution and molecule specificity. In this study, we present an electrochemical nanodevice based on a 0-nanopipette designed to coanalyze calcium ions (Ca2+) and reactive oxygen species (ROS) at a single mitochondrion and its surrounding cytosol, thereby ...
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作者：Dhungel, Sajina; Xiao, Michael; Pushpabai, Rajesh Rajaian; Kikani, Chintan K.
作者单位：Rockefeller University; University of Kentucky
摘要：PAS domains are ubiquitous sensory modules that transduce environmental signals into cellular responses through tandem PAS folds and PAS-associated C-terminal (PAC) motifs. While this conserved architecture underpins their regulatory roles, here we uncover a structural divergence in the metazoan PAS domain-regulated kinase (PASK). By integrating evolutionary-scale domain mapping with deep learning-based structural models, we identified two PAS domains in PASK, namely PAS-B and PAS-C, in additi...
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作者：Fiscella, Kevin
作者单位：University of Rochester
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作者：Gailleton, Romain; Mathew, Nimitha R.; Reusch, Laura; Schon, Karin; Scharf, Lydia; Stromberg, Anneli; Cvjetkovic, Andrea; Aziz, Luaay; Hellgren, Johan; Tang, Ka-Wei; Bemark, Mats; Angeletti, Davide
作者单位：University of Gothenburg; Sahlgrenska University Hospital; Sahlgrenska University Hospital; University of Gothenburg; University of Gothenburg; Sahlgrenska University Hospital; Lund University; SciLifeLab; University of Gothenburg
摘要：The nasal mucosa is the first immunologically active site that respiratory viruses encounter and establishing immunity at the initial point of pathogen contact is essential for preventing viral spread. Influenza A virus (IAV) in humans preferentially replicates in the upper respiratory tract (URT) but mouse models of infection result in lower respiratory tract infection. Here, we optimize IAV inoculation to enhance replication in the nasal turbinate (NT) and study local B cell immunity. We dem...
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作者：Mychack, Aaron; Evans, Dwayne; Gilles, Tarah; James, Michael J.; Walker, Suzanne
作者单位：Harvard University; Harvard Medical School; Harvard University; Harvard University; Harvard Medical School
摘要：Staphylococcus aureus is a Gram-positive pathogen responsible for numerous antibiotic-resistant infections. Identifying vulnerabilities in S. aureus is crucial for developing new antibiotics to treat these infections. With this in mind, we probed the function of GdpS, a conserved Staphylococcal membrane protein containing a cytoplasmic GGDEF domain. These domains are canonically involved in cyclic-di-GMP signaling processes, but S. aureus is not known to make cyclic-di-GMP. Using a transposon ...
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作者：Ruwe, Hannes; Spallek, Thomas
作者单位：University of Gottingen
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作者：Wang, Binhang; Sun, Libo; Wang, Tong; Cao, Yujie; Li, Xiang; Xu, Wenhao; Zhang, Jie; Tang, Yun
作者单位：Fudan University
摘要：Ordered mesoporous Metal-Organic Frameworks (mesoMOFs), integrating mesopores and micropores, address mass transfer limitations characteristic of microporous materials, thus broadening their application spectrum. However, the synthesis of mesoMOFs has been predominantly achievable in aqueous media, rather than in organic solvents, which are more conducive to the growth and stabilization of MOFs. This is primarily attributed to the challenge in forming stable and uniform micelles from block cop...
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作者：Wang, Xiaohan; Liu, Mengyao; Zhang, Yifan; Ma, Dongyuan; Wang, Lu; Liu, Feng
作者单位：Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Beijing Institute for Stem Cell & Regenerative Medicine; Chinese Academy of Medical Sciences - Peking Union Medical College; Peking Union Medical College; Institute of Hematology & Blood Diseases Hospital - CAMS; Shandong University
摘要：During fetal stage, hematopoietic stem and progenitor cells (HSPCs) undergo rapid proliferation with a tight control of genomic stability. Although histone H3 lysine 4 (H3K4) methylation has been reported to stabilize the genome in proliferating cells, its specific role in HSPC development remains elusive. In this study, we demonstrated that tryptophan-aspartic acid (WD) repeat protein 5 (Wdr5)- mediated H3K4 methylation is crucial for maintaining genomic stability of proliferating HSPCs in ze...
