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作者：Pelentritou, Andria; Pfeiffer, Christian; Iten, Manuela; Haenggi, Matthias; Zubler, Frederic; Schwartz, Sophie; De Lucia, Marzia
作者单位：University of Lausanne; Centre Hospitalier Universitaire Vaudois (CHUV); University of Bern; University Hospital of Bern; University of Zurich; University Zurich Hospital; University of Bern; University of Geneva; University of Geneva
摘要：In healthy awake individuals, the neural processing of bodily signals is not only essential for survival but can also influence perception and compete with external stimulus processing. Yet, the mechanism underlying this bidirectional processing of bodily and external stimuli, as well as its persistence or modulation in unconscious states, remains largely unknown. Here, we investigated the role of cardiac activity on auditory regularity processing in coma. We recorded continuous electroencepha...
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作者：Santamaria, Carolina; Biegas, Kyle J.; Lim, Pamelia N.; Cabral, Jessica; Kim, Christi Y.; Lee, James R.; Gaidhane, Ishani, V; Papson, Casey; Gomard- Henshaw, Kyla; Rothchild, Alissa C.; Swarts, Benjamin M.; Siegrist, M. Sloan
作者单位：University of Massachusetts System; University of Massachusetts Amherst; University of Massachusetts System; University of Massachusetts Amherst; Central Michigan University; Central Michigan University; University of Massachusetts System; University of Massachusetts Amherst
摘要：Mycobacterial cell envelopes are rich in unusual lipids and glycans that play key roles during infection and vaccination. The most abundant envelope glycolipid is trehalose dimycolate (TDM). TDM compromises the host response to mycobacterial species via multiple mechanisms, including inhibition of phagosome maturation. The molecular mechanism by which TDM inhibits phagosome maturation has been elusive. We find that a clickable, photoaffinity TDM probe recapitulates key phenotypes of native TDM...
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作者：Rambert, Camille; Nield, Joanna M.; Narteau, Clement; Delorme, Pauline; Wiggs, Giles F. S.; Baddock, Matthew C.; Best, Jim; Christensen, Kenneth T.; Claudin, Philippe
作者单位：Universite PSL; Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS); Universite Paris Cite; University of Southampton; Centre National de la Recherche Scientifique (CNRS); Universite Paris Cite; Universite PSL; Ecole Normale Superieure (ENS); Centre National de la Recherche Scientifique (CNRS); University of Oxford; Loughborough University; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Colorado System; University of Colorado Denver; Children's Hospital Colorado; University of Colorado Anschutz Medical Campus
摘要：Desert surfaces are typically nonuniform, with individual sand dunes generally surrounded by gravel or nonerodible beds. Similarly, beaches vary in composition and moisture that enhances cohesion between the grains. These bed heterogeneities affect the aeolian transport properties greatly and can then influence the emergence and dynamics of bedforms. Here, we propose a model that describes how, due to transport capacity being greater on consolidated than erodible beds, patches of sand can grow...
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作者：Biswas, Rupam; Lopez-Serrano, Ana Laura; Purohit, Apoorva; Ramirez-Navarro, Angelina; Huang, Hsiang-Ling; Grandinetti, Giovanna; Cheng, Xiaolin; Heissler, Sarah M.; Deschenes, Isabelle; Chinthalapudi, Krishna
作者单位：University System of Ohio; Ohio State University; University System of Ohio; Ohio State University; University System of Ohio; Ohio State University; University System of Ohio; Ohio State University; University System of Ohio; Ohio State University
摘要：Voltage- gated Nav1.5 channels are central to the generation and propagation of cardiac action potentials. Aberrations in their function are associated with a wide spectrum of cardiac diseases including arrhythmias and heart failure. Despite decades of progress in Nav1.5 biology, the lack of structural insights into intracellular regions has hampered our understanding of its gating mechanisms. Here, we present two cryo- EM structures of human Nav1.5 in open states, revealing sequential conform...
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作者：Belof, Jonathan L.; Brown, Justin L.; Myint, Philip C.; Sterbentz, Dane M.; Hao, Yue; Stoltzfus, Brian S.; Benedict, Lorin X.
作者单位：United States Department of Energy (DOE); Lawrence Livermore National Laboratory; United States Department of Energy (DOE); Sandia National Laboratories
摘要：Using pulsed-power magnetic field sources to compress gallium to gigapascal pressures on nanosecond timescales, we report here experiments on shockless dynamic compression of a liquid metal. Time-resolved velocimetry data reveal signatures of rapid freezing from a metastable liquid state, and we demonstrate that the kinetics of this nonequilibrium solidification can be accurately simulated with a computational modeling framework we have developed in previous studies, where classical nucleation...
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作者：Engel, Nils W.; Steinfeld, Israel; Ryan, Daniel; Anupindi, Kusala; Kim, Samuel; Wellhausen, Nils; Chen, Linhui; Wilkins, Katherine; Baker, Daniel J.; Rommel, Philipp C.; Jarocha, Danuta; Gohil, Mercy; Zhang, Qian; Milone, Michael C.; Fraietta, Joseph A.; Davis, Megan; Young, Regina M.; June, Carl H.
作者单位：University of Pennsylvania; Agilent Technologies; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania; University of Pennsylvania
摘要：Genome-editing technologies have enabled the clinical development of allogeneic cellular therapies, yet the optimal gene-editing modality for multiplex editing of therapeutic T cell product manufacturing remains elusive. In this study, we conducted a comprehensive comparison of CRISPR/Cas9 nuclease and adenine base editor (ABE) technologies in generating allogeneic chimeric antigen receptor (CAR) T cells, utilizing extensive in vitro and in vivo analyses. Both methods achieved high editing eff...
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作者：Ravindran, Sandeep
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作者：Sneed, Sunny L.; Strandberg, Elizabeth A.; Laureano, Ana F. S.; Vattepu, Ravi; Sun, Yehao; Tran, Truc T.; Conroy, Michelle E.; Anthony, Robert M.
作者单位：Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard Medical School; University of North Carolina; East Carolina University; Amgen
摘要：IgG antibodies are the basis for many successful therapeutics. A single, N-linked glycan is present on the Fc on all IgGs, and the composition of that glycan exerts marked influence over effector functions of the IgG. We and others have shown terminal sialylation of the Fc glycan confers anti-inflammatory activity to the IgG1 subclass and is thought to be responsible for the anti-inflammatory activity of high-dose intravenous immunoglobulin. However, whether sialylation results in anti-inflamm...
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作者：Townrow, Luke A.; Krupenye, Christopher
作者单位：Johns Hopkins University
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作者：Wang, Qiuyi; Wang, Yue; Lin, Yujie; Zhou, Jiawen; Mao, Zhiyuan; Gu, Xin; Chen, Haiyun; Li, Jiyu; Chen, Ao; Zhang, Jin'ge; Wang, Rong; Zhao, Yingming; Gu, Mufeng; Li, Qing; Zhang, Yongjie; Jin, Jianliang
作者单位：Nanjing Medical University; Jiangnan University; Nanjing University
摘要：New immunosenescence targets for preventing senescence-associated pathological cardiac hypertrophy (SA-PCH) need to be explored. In the present study, with physiologically aged human and mouse samples, the IL-17A level increased with physiological aging, heart failure (HF), and SA-PCH and was negatively correlated with thymic Bmi-1 expression. Bmi-1f/fLckCre+ mice and Bmi-1f/flittermates were generated to determine whether Bmi-1 delayed T cell aging by maintaining thymic T cell development to ...
