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作者：Izuma, Keise; Kakinuma, Kyosuke
作者单位：Kochi University Technology; Kochi University Technology; University of Southampton; Japan Society for the Promotion of Science
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作者：Guo, Weijie; Huang, Sihao; Shao, Xiangli; Wu, Yuting; Ma, Yuan; Lu, Shuya; Ren, Hanlin; Zhou, Xuening; Yang, Zhenglin; Lyu, Mingkuan; Liu, Yiwei; Gordon, Vernita; Brodbelt, Jennifer S.; Pan, Tao; Lu, Yi
作者单位：University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin; University of Chicago; University of Texas System; University of Texas Austin; University of Texas System; University of Texas Austin
摘要：Neutrophils release neutrophil extracellular traps (NETs) to neutralize infections, a process that also contributes to immunothrombosis. While beneficial in localized infections, excessive NET formation can lead to widespread coagulopathy and organ failure. While the roles of NET-associated proteins such as histones in immunothrombosis are well characterized, NET-derived DNAs are much less known. To address this issue, we report herein the direct interaction between thrombin and DNA scaffolds ...
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作者：Moresco, Philip; Kastan, Jonathan P.; Yang, Jung- in; Prabakar, Rishvanth; Kelley, Larkin; Minicozzi, Francesca; Adams, Dexter W.; Cifani, Paolo; Tuveson, David A.; Fearon, Douglas T.
作者单位：Cold Spring Harbor Laboratory; State University of New York (SUNY) System; Stony Brook University; State University of New York (SUNY) System; Stony Brook University; Stony Brook University Hospital; Cornell University; Weill Cornell Medicine; Howard Hughes Medical Institute
摘要：The exclusion of T cells causes immune escape of pancreatic ductal adenocarcinoma (PDA). T cell exclusion is mediated by the interaction between CXCR4 on T cells and its ligand, CXCL12, which is complexed to keratin-19 (KRT19) on the surface of PDA cells. KRT19 secretion by PDA cells is essential to this process but is unusual because KRT19 lacks an endoplasmic reticulum (ER)-directing signal peptide (SP). By using biotinylation by an ER-restricted TurboID system and a split-GFP assay in PDA c...
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作者：Park, Youngseon; Sisley, Tyler A.; Stone, Madeleine C.; Corrigan, Rebecca M.; Vickery, Christopher R.; Gruendling, Angelika; Walker, Suzanne
作者单位：University College Dublin; Harvard University; Harvard Medical School; Imperial College London; Imperial College London
摘要：Staphylococcus aureus is a Gram-positive pathogen that causes life-threatening infections. Its cell envelope contains anionic polymers called teichoic acids that are required for cell viability.Teichoic acids come in two forms and are made by different biosynthetic pathways. One form, lipoteichoic acid (LTA), is anchored in the cell membrane; the other form, wall teichoic acid (WTA), is covalently linked to the peptidoglycan cell wall. Although the LTA and WTA biosynthetic pathways have been c...
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作者：Wang, Song; Zhou, Jingwen; Li, Jianghua; Chen, Jian; Du, Guocheng; Li, Zhengqiang; Lee, Sang Yup; Zhao, Xinrui
作者单位：Jiangnan University; Jiangnan University; Jiangnan University; Jiangnan University; Jilin University; Korea Advanced Institute of Science & Technology (KAIST); Korea Advanced Institute of Science & Technology (KAIST)
摘要：Hemoglobins (Hb) and myoglobins (Mb) are important hemoproteins with broad applications in food and medicine. Microbial cell factory is a promising approach for the green and sustainable production of hemoproteins. However, current microbial hosts face the challenges of safety and insufficient heme supply. Here, we report a global regulation strategy, push-restrain-pull-block, to enhance heme supply for producing various active Hb and Mb in food-grade Bacillus subtilis. Initially, the insuffic...
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作者：Guo, Jiawei; Li, Ke; Ming, Yue; Pan, Yitong; Tan, Shuangyan; Ma, Hulin; Chen, Shuang; Duan, Yingying; Peng, Yong
作者单位：Sichuan University; Sichuan University; Tianfu Jincheng Laboratory
摘要：Bromodomain-and-extraterminal-domain (BET) proteins are promising therapeutic targets for refractory solid tumors, including triple-negative breast cancer (TNBC). However, acquired resistance to BET inhibitors (BETi) remains a significant clinical challenge. Elucidation of the underlying mechanisms of BETi resistance is therefore of critical importance. In this study, we identified the RNA-binding protein IGF2BP2 as a key driver of acquired BETi resistance in TNBC, primarily through its role i...
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作者：Chen, Chuqiao; Narvaez, Carina D. V. Martinez; Chang, Nina; Jimenez, Carlos Medina; Dennis, Joseph M.; Jaeger, Heinrich M.; Rowan, Stuart J.; de Pablo, Juan J.
作者单位：University of Chicago; United States Department of Defense; United States Army; US Army Research, Development & Engineering Command (RDECOM); US Army Research Laboratory (ARL); University of Chicago; University of Chicago; University of Chicago; United States Department of Energy (DOE); Argonne National Laboratory; New York University; New York University; New York University
摘要：The morphological features of particles, notably shape anisotropy, critically influence the rheological properties of dense suspensions, spanning both natural and engineered systems. This work explores the potential of using shape memory particles to dynamically regulate suspension fluid flow through controllable shape transformations. First, we synthesize shape-memory particles with programmable anisotropy from liquid crystal elastomers, such that the stiffness and shapes of the particles can...
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作者：Dooling, Lawrence J.; Anlas, Alisya A.; Tobin, Michael P.; Ontko, Nicholas M.; Marchena, Tristan; Wang, Maximilian; Andrechak, Jason C.; Discher, Dennis E.
作者单位：University of Pennsylvania; University of Houston System; University of Houston
摘要：Macrophages often pervade solid tumors, and clusters of macrophages sometimes associate with longer survival of patients. However, clustering mechanisms and impacts on key functions such as phagocytosis remain obscure. Here, under conditions that maximize cancer cell phagocytosis within cohesive tumors, we uncover pathways that favor dynamic clusters and find a colocalization of tumor-intrusive pseudopodia which we term intrudopodia. Cluster formation over hours on low-adhesion substrates occu...
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作者：Liu, Bin; Su, Yingying; Sun, Hao; Yang, Bin; Wan, Lili; Li, Xiaoya; Hou, Shaobin; Ma, Guozhen; Yu, Juan Joanna; Feng, Lu; Li, Huamin Henry; Wang, Lei
作者单位：Nankai University; Nankai University
摘要：Transcytosis across the blood-brain barrier (BBB), composed of brain microvascular endothelial cells (HBMECs), is tightly controlled to prevent the entry of macromolecules, microorganisms, and toxins into the brain. Transferrin receptor (TfR) transcytosis, one of the few active transcytosis pathways in HBMECs, is extensively utilized for drug transport across the BBB and employed by meningitis-causing bacteria to penetrate into the brain. However, the molecular mechanism facilitating the fusio...
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作者：Nie, Yufeng; Chen, Jianli; Xu, Guodong; Loecher, Anno
作者单位：Hong Kong Polytechnic University; Hong Kong Polytechnic University; Hong Kong Polytechnic University; University of Bonn
摘要：Using time-variable gravity fields determined from satellite laser ranging (SLR) and the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On (GRACE-FO), we derive three decades of barystatic sea level (BSL) change estimates since 1993, providing a long record of satellite gravimetry-based BSL estimates comparable in duration to satellite altimetry.Over the 1993-2022 altimetry era, the BSL change rate is 1.75 +/- 0.59 mm/y (2 sigma) as observed by SLR. The rate increases to 2.16...
