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作者：Ma, Qian; Liu, Sijia; Doyle, Siamsa M.; Raggi, Sara; Parizkova, Barbora; Barange, Deepak Kumar; Ratnakaram, Hemamshu; Wilkinson, Edward G.; Garcia, Isidro Crespo; Bygdell, Joakim; Wingsle, Gunnar; Boer, Dirk Roeland; Strader, Lucia C.; Almqvist, Fredrik; Novak, Ondrej; Robert, Stephanie
作者单位：Swedish University of Agricultural Sciences; Umea University; Palacky University Olomouc; Czech Academy of Sciences; Institute of Experimental Botany of the Czech Academy of Sciences; Umea University; Duke University; Umea University; Ghent University; Ghent University; Flanders Institute for Biotechnology (VIB); Shenyang Agricultural University; Swedish University of Agricultural Sciences; Umea University; Colorado State University System; Colorado State University Fort Collins
摘要：Apical hook development is an ideal model for studying differential growth in plants and is controlled by complex phytohormonal crosstalk, with auxin being the major player. Here, we identified a bioactive small molecule that decelerates apical hook opening in Arabidopsis thaliana. Our genetic studies suggest that this molecule enhances or maintains the auxin maximum found in the inner hook side and requires certain auxin signaling components to direct target of this compound. We present data ...
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作者：Rekhi, Shiv; Mittal, Jeetain
作者单位：Texas A&M University System; Texas A&M University College Station; Texas A&M University System; Texas A&M University College Station; Texas A&M University System; Texas A&M University College Station
摘要：The self-assembly of intrinsically disordered proteins into biomolecular condensates depends on their primary sequence, leading to sequence-dependent phase separation. Computational methods to study this behavior often rely on residue-level interaction potentials that estimate the propensity of amino acids to partition between the dilute and dense phases. While distribution coefficients would provide the most direct measure of these potentials, their unavailability has led to the use of proxie...
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作者：Till, Katharina; Seinen, Anne-Bart; Wruck, Florian; Sunderlikova, Vanda; Galmozzi, Carla V.; Katranidis, Alexandros; Bukau, Bernd; Kramer, Guenter; Tans, Sander J.
作者单位：AMOLF; Helmholtz Association; German Cancer Research Center (DKFZ); Consejo Superior de Investigaciones Cientificas (CSIC); University of Sevilla; CSIC-JA-USE - Instituto de Biomedicina de Sevilla (IBIS); Delft University of Technology
摘要：Conformational control of nascent chains is poorly understood. Chaperones are known to stabilize, unfold, and disaggregate polypeptides away from the ribosome. In comparison, much less is known about the elementary conformational control mechanisms at the ribosome. Yet, proteins encounter major folding and aggregation challenges during translation. Here, using selective ribosome profiling and optical tweezers with correlated single- molecule fluorescence, with dihydrofolate reductase (DHFR) as...
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作者：Cook, Cyril; Ramsey, Kristen M.; Barrick, Doug
作者单位：Johns Hopkins University; University of Connecticut
摘要：The Notch signaling pathway regulates cellular differentiation by activating transcription through an unusual heterotrimeric complex comprising the Notch receptor's intracellular domain (NICD), the DNA-binding protein CSL, and the coactivator MAML. NICD has two binding sites for CSL, a short motif in the RAM region and an ankyrin domain (ANK), connected by an intrinsically disordered linker and which form a bivalent ternary complex with CSL and MAML. Although bivalency is required for maximal ...
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作者：Nemhauser, Jennifer L.; Weigel, Detlef
作者单位：University of Washington; University of Washington Seattle
摘要：Joanne Chory (1955-2024) was a pioneering biologist whose research transformed our understanding of plant growth and development. Her groundbreaking work, based on many clever genetic screens, established a complete signaling pathway for brassinosteroids, from the biosynthesis of this important class of plant hormones and their transmembrane receptor to cytoplasmic and nuclear effectors. A beloved mentor and visionary leader, Chory championed science as both a rigorous discipline and a force f...
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作者：Piekarski, Patrick K.; Valdes-Rodriguez, Stephany; Trible, Waring; Kronauer, Daniel J. C.
作者单位：Rockefeller University; Howard Hughes Medical Institute; Rockefeller University; Harvard University
摘要：Polyphenic traits in animals often exhibit nonlinear scaling with body size. Static allometries (i.e., scaling relationships) themselves can exhibit plasticity, such that individuals of the same size and genotype differ in body proportions across different environments. In ants, both larval environment and genotype regulate the expression of caste- associated traits, including body size and ovariole number. However, it remains untested whether caste- associated traits are independently regulat...
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作者：Wang, Feng; He, Qing; O'Donnell, Michael E.; Li, Huilin
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作者：Ling, Jing; Zhang, Yuan; Hei, Yongzhen; Kompa, Julian; Yang, Chen; Wang, Bo; Zhang, Junwei; Du, Jiasheng; Rudi, Tatjana; Zhang, Kecheng; Sun, Jingfu; Wang, Wenjuan; Fabritz, Sebastian; Li, Yulong; Deng, Wulan; Zou, Peng; Chen, Chunlai; Chen, Zhixing
作者单位：Peking University; Peking University; Tsinghua University; Max Planck Society; Peking University; Peking University; Max Planck Society; Tsinghua University; Peking University; Peking University; Chinese Institute for Brain Research, Beijing; Peking University; Chinese Academy of Sciences
摘要：Self- labeling protein tags are widely used in advanced bioimaging where dyes with high- photon budgets outperform their fluorescent protein counterparts. Further increasing the emitted photon numbers of dye- tag systems is actively pursued by both new fluorophore chemistry and protein engineering. By scrutinizing the protein microenvironment of fluorophores, here we propose that proximal thioether groups negatively affect the photostability of the dye- tag system. We attribute the disparity i...
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作者：Dewey, James B.
作者单位：University of Southern California
摘要：Mammalian hearing sensitivity depends on the amplification of sound-induced cochlear vibrations by outer hair cells (OHCs). OHCs transduce deflections of their stereociliary bundles into receptor potentials that drive changes in cell length. While fast, phasic OHC length changes are thought to generate the forces that underlie cochlear amplification, OHCs also exhibit large tonic length changes in response to sound. These tonic length changes could theoretically arise from asymmetries in the m...
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作者：Fratus, Marco; Alam, Muhammad A.
作者单位：Purdue University System; Purdue University
摘要：Since the 1960s, enzymatic sensors have been vital in healthcare and environmental monitoring due to their high selectivity. Traditionally, their performance is interpreted using the Michaelis-Menten (MM) equation, which assumes idealized, homogeneous, well-mixed laboratory conditions. However, integrating these sensors with microneedle (MN) patches for wearable applications introduces challenges such as spatial and temporal variations and limited reactant availability. Applying the MM model i...
