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作者：Yamanashi, Taro; Muraoka, Yuki; Furuta, Tadaomi; Kume, Tsukasa; Sekido, Natsuko; Saito, Shunya; Terashima, Shota; Yokoyama, Takeshi; Tanaka, Yoshikazu; Miyamoto, Atsushi; Sato, Kanane; Ito, Tomoyuki; Nakazawa, Hikaru; Umetsu, Mitsuo; Tanudjaja, Ellen; Tsujii, Masaru; Dreyer, Ingo; Schroeder, Julian I.; Ishimaru, Yasuhiro; Uozumi, Nobuyuki
作者单位：Tohoku University; Tohoku University; Universidad de Talca; University of California System; University of California San Diego
摘要：Guard cells, which regulate stomatal apertures in plants, possess a sophisticated mechanism for regulating turgor pressure. The outward- rectifying K+out channel GORK, expressed in guard cells of the plant Arabidopsis thaliana, is a central component that promotes stomatal closure by releasing K+ to the extracellular space, thereby lowering turgor pressure. To date, the structural basis underlying the regulation of the K+ transport activity of GORK is unclear. Using cryo- EM, we determined the...
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作者：Ai, Qian; Bonagiri, Lalith Krishna Samanth; Panse, Kaustubh S.; Kim, Jaehyeon; Zhou, Shan; Zhang, Yingjie; Cui, Yi
作者单位：University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign; University of Illinois System; University of Illinois Urbana-Champaign
摘要：In electrochemical systems, the structure of electrical double layers (EDLs) near electrode surfaces is crucial for energy conversion and storage functions. While the electrodes in real-world systems are usually heterogeneous, to date the investigation of EDLs is mainly limited to planar, homogeneous substrates. To bridge this gap, here we image the EDL structure of an ionic liquid/graphite battery anode system in the initial stage of interfacial nucleation and growth using our recently develo...
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作者：Boyer, Jacob A.; Rosen, Ezra Y.; Sharma, Malvika; Dorso, Madeline A.; Mai, Nicholas; Amor, Corina; Reiter, Jason M.; Kannan, Ram; Gadal, Sunyana; Xu, Jianing; Miele, Matthew; Li, Zhuoning; Chen, Xiaoping; Chang, Qing; Pareja, Fresia; Worland, Stephan; Warner, Douglas; Sperry, Sam; Chiang, Gary G.; Thompson, Peggy A.; Yang, Guangli; Ouerfelli, Ouathek; Drilon, Alexander; de Stanchina, Elisa; Wendel, Hans- Guido; Chandarlapaty, Sarat; Rosen, Neal
作者单位：Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Ludwig Institute for Cancer Research; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Memorial Sloan Kettering Cancer Center; Cornell University; Weill Cornell Medicine
摘要：Most breast cancers depend on hormone-stimulated estrogen receptor alpha (ER) activity and are sensitive to ER inhibition. Resistance can arise from activating mutations in the gene encoding ER (ESR1) or from reactivation of downstream targets. Newer ER antagonists occasionally show efficacy but are largely ineffective as single agents in the long term. Here, we show that ER translation is eIF4E/cap-independent yet sensitive to inhibitors of the translation initiation factor eIF4A. EIF4A inhib...
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作者：Lin, Yiyan; Parajon, Eleana; Yuan, Qinling; Ye, Siyu; Qin, Guanghui; Deng, Yu; Borleis, Jane; Koyfman, Ariel; Iglesias, Pablo A.; Konstantopoulos, Konstantinos; Robinson, Douglas N.; Devreotes, Peter N.
作者单位：Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins University; Microsoft; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine; Johns Hopkins University; Johns Hopkins Medicine
摘要：Ras has traditionally been regarded as a positive regulator and therapeutic target due to its role in cell proliferation, but recent findings indicate a more nuanced role in cell migration, where suppressed Ras activity can unexpectedly promote migration. To clarify this complexity, we systematically modulate Ras activity using various RasGEF and RasGAP proteins and assess their effects on migration dynamics. Leveraging optogenetics, we assess the immediate, nontranscriptional effects of Ras s...
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作者：Soom, Sarah; Moning, Stefan Urs; Cook, Gregory M.; Lingford, James P.; Kropp, Ashleigh; Tran, Sieu; Grinter, Rhys; Greening, Chris; von Ballmoos, Christoph
作者单位：University of Bern; University of Bern; University of Otago; Queensland University of Technology (QUT); Monash University; Monash University; University of Melbourne; Monash University; Monash University
摘要：All cells require a continuous supply of the universal energy currency, adenosine triphosphate (ATP), to drive countless cellular reactions. The universally conserved F1Fo-ATP synthase regenerates ATP from ADP and Pi by harnessing a transmembrane electrochemical proton gradient (pmf). Bacteria have evolved diverse pmf-forming strategies using light, organic, and inorganic energy sources. Recently, we proposed that many bacteria survive using atmospheric trace gases to produce ATP when limited ...
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作者：Tang, Weiwei; Yang, Taimin; Tu, Qing; Calderon, Hector A.; Hernandez, Francisco C. Robles; O'Reilly, Padraic; Jing, Yun; Park, Sung; Rimer, Jeffrey D.
作者单位：University of Houston System; University of Houston; Tianjin University; Stockholm University; Texas A&M University System; Texas A&M University College Station; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory
摘要：Understanding and exploiting material flexibility through phenomena such as the bending and twisting of molecular crystals has been a subject of increased interest owing to the number of applications that benefit from these properties, such as optoelectronics, mechanophotonics, soft robotics, and smart sensors. Here, we report the growth of spontaneously bent and twisted ammonium urate crystals induced by the keto-enol tautomerism of the urate molecule. The major tautomer is native to biogenic...
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作者：Feng, Xiang; Schut, Gerrit J.; Putumbaka, Saisuki; Li, Huilin; Adams, Michael W. W.
作者单位：Van Andel Institute; University System of Georgia; University of Georgia; California Institute of Technology
摘要：Members of the tungsten-containing oxidoreductase (WOR) family, which contain a tungstopyranopterin (Tuco) cofactor, are typically either monomeric (WorL) or heterodimeric (WorLS). These enzymes oxidize aldehydes to the corresponding acids while reducing the redox protein ferredoxin. They have been structurally characterized mainly using WORs from hyperthermophilic archaea. The WORs of some bacteria contain three additional subunits of the BfuABC family and these chimeric WorABCSL enzymes cata...
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作者：Gao, Forson; Ye, Fuzhou; Jovanovic, Milija; Buck, Martin; Zhang, Xiaodong
作者单位：Imperial College London; Imperial College London; University of Belgrade; Francis Crick Institute
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作者：Halford, Julia; Senatore, Amanda J.; Berryman, Sage; Semidey, Destinee; Munoz, Antonio; Doan, Ryan A.; Coombs, Adam M.; Noimany, Brandon; Emberley, Katie; Emery, Ben; Monk, Kelly R.; Murthy, Swetha E.
作者单位：Oregon Health & Science University; Oregon Health & Science University
摘要：Infantile hypomyelinating leukodystrophy 19 (HLD19) is a rare genetic disorder where patients exhibit reduced myelin in central nervous system (CNS) white matter tracts and present with varied neurological symptoms. The causative gene TMEM63A encodes a mechanosensitive ion channel whose role in myelination is largely unexplored. Our study shows that TMEM63A is a major regulator of oligodendrocyte (OL)-dependent myelination in the CNS. In mouse and zebrafish, Tmem63a inactivation led to early d...
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作者：Potapov, Peter; Tyukavina, Alexandra; Turubanova, Svetlana; Hansen, Matthew C.; Giglio, Louis; Hernandez-Serna, Andres; Lima, Andre; Harris, Nancy; Stolle, Fred
作者单位：University System of Maryland; University of Maryland College Park
摘要：Global forests provide key ecosystem services, from climate regulation to biodiversity habitat, but are under increasing pressure from the combined impacts of climate and land use change. Here, we show that forest disturbance due to fire is growing globally, with the most dramatic increases in intact forest landscapes, highlighting an existential threat to remaining high biomass, high biodiversity forests. The global annual area of forest disturbance due to fire for 2023 and 2024 was highest s...
