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作者：Boston, Rebecca S.; Marks, M. David; Cohen, Jerry D.
作者单位：North Carolina State University; University of Minnesota System; University of Minnesota Twin Cities; University of Minnesota System; University of Minnesota Twin Cities
摘要：Brian Larkins died in January 2025 at the age of 78 leaving a legacy of seminal scientific contributions that were fundamental to the establishment of the field of plant molecular biology. He elucidated the key processes in maize endosperm development and delivered technical breakthroughs in isolation of polysomes and translation of associated messenger RNAs. His models for protein body structure and his work to understand the molecular basis for restoring transparency to mutant maize seeds pr...
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作者：Brodsky, Nina N.; Chakder, Monisha; Kumar, Dinesh Babu Uthaya; Barmada, Anis; Wang, Julia; Gu, Weihong; Olaloye, Oluwabunmi; Ramaswamy, Anjali; Wats, Aanchal; Konnikova, Liza; Lucas, Carrie L.
作者单位：Yale University; Yale University; Yale University; Yale University
摘要：Neonates and infants are distinct in their clinical and cellular responses to viral infections, with neonatal CD8+ T cells displaying innate-like characteristics and a low threshold for T cell receptor activation. However, specific molecular programs that drive these unique responses are incompletely understood, particularly in humans, and targetable pathways to modulate viral illness in this vulnerable population remain to be elucidated. Early-life immune responses may be developmentally prog...
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作者：Tobias, Joseph A.; Bullock, James M.; V. Dicks, Lynn; Forester, Brenna R.; Razgour, Orly
作者单位：Imperial College London; UK Centre for Ecology & Hydrology (UKCEH); University of Cambridge; United States Department of the Interior; US Fish & Wildlife Service; University of Exeter
摘要：Conservation science and policy are geared primarily toward the preservation of species and habitats, with priority often given to the rarest, most vulnerable or most charismatic forms. This pattern-based approach has broad appeal and offers a pragmatic short-cut for targeting conservation action. However, the long-term efficacy of species and landscape conservation programs remains highly uncertain, amid growing evidence that sustainable conservation action requires an increased emphasis on p...
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作者：Hardwick, Jack S.; Dhir, Somdutta; Kirchner, Angie; Simeone, Angela; Flynn, Sean M.; Edgerton, James M.; Tavares, Rafael de Cesaris Araujo; Garcia, Isabel Esain -; Tannahill, David; Golder, Paula; Monahan, Jack M.; Gosal, Walraj S.; Balasubramanian, Shankar
作者单位：University of Cambridge; CRUK Cambridge Institute; University of Cambridge; Cancer Research UK; University of Cambridge; University of Bristol; University of Exeter
摘要：In mammalian genomes, cytosine modifications form a layer of regulatory information alongside the genetic code. Decoding this information is crucial to our understanding of biology and disease. Established sequencing methods cannot simultaneously resolve cytosine's three most common forms-cytosine (C), 5-methylcytosine (mC), and 5-hydroxymethylcytosine (hmC)-across both strands of the DNA double helix. Thus, how epigenetic information is distributed in DNA remains unclear. Here, we present Str...
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作者：Malatesta, Marco; De Rito, Carlo; Gasparini, Francesca; Merici, Giovanni; Dell'Accantera, Davide; Quilici, Giacomo; Sansone, Francesco; Percudani, Riccardo
作者单位：University of Parma; University of Parma
摘要：Excretion of L-xylulose is the hallmark of pentosuria, the fourth of Garrod's inborn errors of metabolism, yet the molecular basis for L-xylulose formation remains unknown. Here, by projecting coevolutionary data for 511,114 orthogroups across 1,929 eukaryotic genomes onto metabolic maps, we screen for unmapped genes in human metabolism. Among these, we show that the DUF1907 domain of C11orf54 catalyzes formation of L-xylulose by establishing a zinc-coordinated Michaelis complex with beta- ket...
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作者：Jang, Sung-Soo; Agranonik, Nicole; Huguenard, John R.
作者单位：Stanford University
摘要：Carbamazepine (CBZ) is a widely used antiepileptic drug effective in managing partial and generalized tonic-clonic seizures. Despite its established therapeutic efficacy, CBZ has been reported to worsen seizures in another form of epilepsy, generalized absence seizures, in both clinical and experimental settings. In this study, we focused on thalamic reticular (RT) neurons, which regulate thalamocortical network activity in absence seizures, to investigate whether CBZ alters their excitability...
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作者：Gabard-Durnam, Laurel J.; McCormick, Sarah A.; Underwood, Ellen; Tao, Alice; Kim, Isabelle; Coffman, Siobhan; Benster, Lindsay; Graziano, Maria; Nelson, Charles A.; Berde, Charles B.; Cornelissen, Laura; Hensch, Takao K.
作者单位：Northeastern University; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Boston Children's Hospital; University of Tokyo; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Boston Children's Hospital; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Boston Children's Hospital; Harvard University
摘要：How human brain function is established through protracted trajectories of development is not yet fully understood. Maturation of gamma- aminobutyric acid (GABA) circuits drives critical periods of cortical development in animal models. Whether early functional inhibition similarly impacts the pace of human brain development remains unknown. Here, in a longitudinal study of 93 infants across a range of repeated exposures to general anesthesia shortly after birth, we observed a dramatically acc...
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作者：Lahiri, Hiya; Israeli, Eitan; Krugliak, Miriam; Basu, Kingshuk; Britan-Rosich, Yelena; Yaish, Tamar Ravins; Arkin, Isaiah T.
作者单位：Hebrew University of Jerusalem; Hebrew University of Jerusalem; Hebrew University of Jerusalem; City University of Hong Kong; City University of Hong Kong
摘要：Influenza represents a significant threat with seasonal epidemics that can transition to global pandemics, and cross-species infection presenting a continuous challenge. While vaccines and several antiviral drugs are available, constant genetic changes vitiate these prevention and treatment options. Consequently, we decided to search for inhibitors against one of the virus's validated drug targets, its M2 channel that is blocked by aminoadamantanes. Regrettably, widespread mutations in M2 abol...
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作者：Rose, Kevin; Herrmann, Eric; Kakudji, Eve; Lizarrondo, Javier; Celebi, A. Yasemin; Wilfling, Florian; Lewis, Samantha C.; Hurley, James H.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; Max Planck Society; University of California System; University of California Berkeley
摘要：Defective mitochondrial quality control in response to loss of mitochondrial membrane polarization is implicated in Parkinson's disease by mutations in PINK1 and PRKN. Parkin-expressing U2 osteosarcoma (U2OS) cells were treated with the depolarizing agents oligomycin and antimycin A (OA) and subjected to cryo-focused ion beam milling and in situ cryo-electron tomography. Mitochondria were fragmented and devoid of matrix calcium phosphate crystals. Phagophores were visualized, with bridge-like ...
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作者：Sivelli, Giulia; Barakat, Arthur; Marable, Kathryn B.; Gruet, Guillaume; Bitetti, Serena L.; Behr, Barry; Lodde, Valentina; Luciano, Alberto Maria; Herrera, Carolina; Blom, Michelle; Grisi, Marco
作者单位：Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; Stanford University; Stanford University; University of Milan; University of Lausanne; Centre Hospitalier Universitaire Vaudois (CHUV)
摘要：Analyzing cellular health and metabolism without compromising cell integrity is a major challenge. We present a noninvasive technique using micro magnetic resonance spectroscopy (micro MRS) for nondestructive metabolic fingerprinting at the single-cell scale. This is an application of micro MRS to bovine preimplantation embryos (similar to 8 cells) and oocytes (single cell), with measurements performed on a total of over 150 samples. Among various applications, this method holds significant po...
