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作者：Luan, Qing; Clark, Patricia L.
作者单位：University of Notre Dame
摘要：Our current understanding of protein folding is based predominantly on studies of small (<150 aa) proteins that refold reversibly from a chemically denatured state. However, as protein length increases so does the competition between off-pathway misfolding and on-pathway folding, creating a more complex energy landscape (folding funnel). Little is known about how intermediates populated during the folding of larger proteins affect navigation of this more complex landscape. Previously, we repor...
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作者：Ferraro, Kristy M.; Ferraro, Anthony L.; Monk, Julia D.; Forbes, Elizabeth S.
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作者：Sonani, Ravi R.; Liu, Ying; Xiang, Jialin; Cvirkaite-Krupovic, Virginija; Du, Shishen; Chen, Xiangdong; Krupovic, Mart; Egelman, Edward H.
作者单位：University of Virginia; Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; Wuhan University; Wuhan University; Wuhan University
摘要：Diverse extracellular filaments present on the surface of archaea mediate multiple key processes, such as motility, adhesion, and biofilm formation. Although several archaeal filament types have been characterized in considerable detail, many remain understudied, particularly those utilizing noncanonical secretion systems. Here, we describe the Tafi bundling pilus that facilitates biofilm formation in the haloarchaeon Natrinema sp. J7-2. Unlike previously characterized archaeal pili, Tafi is s...
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作者：Cypranowska, Caroline A.; Feldthouse, Maya; Choi, Yoon Gi; Bakshinska, Dariya; Li, Rachel; Newman, Zachary L.; Isacoff, Ehud Y.
作者单位：University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; Francis Crick Institute; University of California System; University of California San Diego; University of Minnesota System; University of Minnesota Twin Cities
摘要：Weakening of synaptic transmission at the Drosophila larval neuromuscular junction triggers two forms of homeostatic compensation, one that increases the probability of glutamate release per action potential (Pr) and another that increases motoneuron (MN) activity. We investigated the molecular changes in MNs that underlie the increase in MN activity. RNA sequencing (RNA-seq) analysis on MNs whose glutamate release is weakened by knockdown of components of the MN transmitter release machinery ...
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作者：Dvir, Gony; Xing, Zenan; Beldman, Irina; Rivera, Andres; Wheeldon, Ian; Cutler, Sean R.
作者单位：University of California System; University of California Riverside; University of California System; University of California Riverside; University of California System; University of California Riverside; Universidad Nacional Autonoma de Mexico; University of California System; University of California Riverside; University of California System; University of California Riverside
摘要：Methods for fast and inexpensive gene synthesis from oligonucleotide pools enable rapid iteration of genetic designs. Here, we describe iggypop (indexed Golden Gate gene assembly from PCR- amplified oligonucleotide pools), a simple computational-experimental pipeline that allows for low- cost design and synthesis of hundreds of genes from oligonucleotide pools using Golden Gate assembly methods. We used iggypop to synthesize a series of single- transcript autonomously bioluminescent reporters ...
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作者：Hoskova, Michaela; Kotov, Oleg, V; Kucukoz, Betul; Murphyc, Catherine J.; Shegaia, Timur O.
作者单位：Chalmers University of Technology; Brno University of Technology; University of Illinois System; University of Illinois Urbana-Champaign
摘要：Self-assembly (SA) plays a pivotal role in nanotechnology, offering cost-effective methods for bottom-up fabrication and providing versatile model systems for investigating fundamental interactions in various bioinspired systems. However, current methods for investigating and quantifying the dynamics of SA systems are limited in their applicability to planar interfaces, particularly in liquid environments. These methods typically rely on analyzing the collective behavior of particle suspension...
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作者：Huang, Yida; Lin, Huimin; Shi, Jiahao; Chen, Yifan; Sang, Qi; Wang, Ruimin; Liu, Wanshan; Wu, Jiao; Li, Yanyan; Xu, Xiaoyu; Ding, Chunmeng; Yang, Shouzhi; Zhang, Juxiang; Jia, Renbing; Fan, Xianqun; Qian, Kun; Zhou, Yixiong
作者单位：Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University; Shanghai Jiao Tong University
摘要：Ocular adnexal lymphoma (OAL) is the most common orbital malignancy in adults. Advanced tools for precise diagnosis and prognosis of OAL are in demand. Here, the nanoparticle-enhanced laser desorption/ionization mass spectrometry was applied for the construction of OAL-associated serum metabolic patterns (SMPs) from 239 participants (104 OAL and 135 non-OAL). Through machine learning, the diagnostic performance with an area-under-the-curve (AUC) of 0.901 was achieved for OAL based on SMPs. Fur...
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作者：Owens, Gregory L.; Cai, Zhe; Bercovich, Natalia; Todesco, Marco; Lee-Yaw, Julie A.; Rieseberg, Loren H.
作者单位：University of Victoria; University of British Columbia; University of British Columbia; University of British Columbia; University of British Columbia; University of Ottawa
摘要：The cytoplasmic genomes of plants and animals often fail to track species boundaries. However, the mechanisms responsible for such patterns are poorly understood, in part because few studies have linked cytoplasmic variation to phenotypic traits or environmental differences. Here, we use 1,554 previously published and 185 new whole genome sequences representing 14 taxa from the sunflower genus Helianthus, 91 phenotypic traits measured in common gardens, and 39 environmental variables to test f...
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作者：Jarvela, Matti; Kananen, Janne; Helakari, Heta; Korhonen, Vesa; Huotari, Niko; Vayrynen, Tommi; Hautamaki, Katariina; Raitamaa, Lauri; Tuunanen, Johanna; Kallio, Mika; Piispala, Johanna; Ansakorpi, Hanna; Kiviniemi, Vesa
作者单位：University of Oulu; University of Oulu; University of Oulu; University of Oulu; University of Oulu; University of Oulu
摘要：Sleep promotes cerebrospinal fluid (CSF) to interstitial fluid (ISF) exchange in the brain facilitated by brain pulsations. Especially brain vasomotion and arterial pulsations modulated by noradrenaline drive the intracranial fluid dynamics. Narcolepsy type 1 (NT1) entails lessened orexinergic output to wake- promoting systems including the noradrenergic locus coeruleus. As arousal state and noradrenergic signaling affect CSF- ISF clearance, we chose patients with NT1 as a human orexin- target...
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作者：Murphy, Daniel M.; Abou-Ghanem, Maya; Ahern, Adam T.; Brock, Charles A.; Cziczo, Daniel J.; Hintsa, Eric J.; Jacquot, Justin L.; Lawler, Michael J.; Lyu, Ming; Moore, Fred L.; Robinson, Michael A.; Roberts, James M.; Schill, Gregory P.; Shen, Xiaoli; Thornberry, Troy D.; Veres, Patrick R.
作者单位：National Oceanic Atmospheric Admin (NOAA) - USA; Purdue University System; Purdue University; University of Colorado System; University of Colorado Boulder; National Oceanic Atmospheric Admin (NOAA) - USA; TOFWERK AG; National Science Foundation (NSF); National Center Atmospheric Research (NCAR) - USA
摘要：Perchlorate is a toxic, regulated contaminant in drinking water. According to previous isotopic studies, much of the perchlorate deposited to the Earth's surface is formed in the atmosphere, with 36Cl suggesting a large contribution from the stratosphere. Here, we present measurements of perchlorate in stratospheric aerosol particles and confirm that the stratosphere is an important source of perchlorate, whereas we did not observe production in the troposphere. Mass mixing ratios of aerosol p...
