Micro magnetic resonance spectroscopy for noninvasive metabolic screening of mammalian embryos and oocytes

• 成果类型: Article
• 署名作者: Sivelli, Giulia; Barakat, Arthur; Marable, Kathryn B.; Gruet, Guillaume; Bitetti, Serena L.; Behr, Barry; Lodde, Valentina; Luciano, Alberto Maria; Herrera, Carolina; Blom, Michelle; Grisi, Marco
• 署名单位: Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne; Stanford University; Stanford University; University of Milan; University of Lausanne; Centre Hospitalier Universitaire Vaudois (CHUV)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12187
• DOI: 10.1073/pnas.2424459122
• 发表日期: 2025-08-05
• 关键词: fatty-acids; visible lipids; beta-oxidation; maturation; disease; h-1

摘要

Analyzing cellular health and metabolism without compromising cell integrity is a major challenge. We present a noninvasive technique using micro magnetic resonance spectroscopy (micro MRS) for nondestructive metabolic fingerprinting at the single-cell scale. This is an application of micro MRS to bovine preimplantation embryos (similar to 8 cells) and oocytes (single cell), with measurements performed on a total of over 150 samples. Among various applications, this method holds significant potential for assisted reproductive technologies (ART), where metabolic assessments of preimplantation embryos could improve treatment outcomes. Early results indicate that classification models using micro MRS data effectively distinguish embryos with high developmental potential and show correlation with oocytes maturity. Furthermore, a multigenerational safety study in a mouse model revealed no adverse effects from embryo exposure to static magnetic field. These findings indicate that micro MRS is a promising, safe tool for assessing embryo metabolism, potentially improving the efficiency and outcomes of ART.