31期

最新文章

• PyReconstruct: A fully open- source, collaborative successor to Reconstruct AI翻译
  作者：Chirillo, Michael A.; Falco, Julian N.; Musslewhite, Michael D.; Lindsey, Larry F.; Harris, Kristen M.
  作者单位：University of Texas System; University of Texas Austin
  摘要：As the serial section community transitions to volume electron microscopy, tools are needed to balance rapid segmentation efforts with documenting the fine detail of structures that support cell function. New annotation applications should be accessible to users and meet the needs of the neuroscience and connectomics communities while also being useful across other disciplines. Issues not currently addressed by a single, modern annotation application include 1) built-in curation systems with u...
• Structural basis of auxin binding and transport by Arabidopsis thaliana AUX1 AI翻译
  作者：Jing, Dan; Kong, Fang; Lu, Xiaoli; Huang, Gaoxingyu; Huang, Jing; Wang, Haolin; Shi, Yigong; Wang, Chengcheng
  作者单位：Zhejiang University; Westlake University; Westlake Laboratory; Tsinghua University
  摘要：Indole-3-acetic acid (IAA), the major form of auxin, is essential for plant growth. Auxin resistant 1 (AUX1), the first identified auxin importer, plays a crucial role in polar auxin transport (PAT). Here, we present cryo-EM structures ofArabidopsis thaliana AUX1 in the IAA-free and IAA-bound states. AUX1 exists as a monomer that contains 11 trans-membrane helices (TMs). TMs 1 to 5 and 6 to 10 constitute the two halves of a classic LeuT-fold, and TM11 interacts with both halves at the interfac...
• Physics-tailored machine learning reveals unexpected physics in dusty plasmas AI翻译
  作者：Yu, Wentao; Abdelaleem, Eslam; Nemenman, Ilya; Burton, Justin C.
  作者单位：Emory University; Emory University
  摘要：Dusty plasma is a mixture of ions, electrons, and macroscopic charged particles that is commonly found in space and planetary environments. The particles interact through Coulomb forces mediated by the surrounding plasma, and as a result, the effective forces between particles can be nonconservative and nonreciprocal. Machine learning (ML) models are a promising route to learn these complex forces, yet their structure should match the underlying physical constraints to provide useful insight. ...
• The power of coalescent methods for inferring recent and ancient gene flow in endangered Bactrian camels AI翻译
  作者：Zhu, Tianqi; Wang, Zhen; Yang, Ziheng
  作者单位：Chinese Academy of Sciences; Chinese Academy of Sciences; Shanghai Institute of Nutrition & Health, CAS; University of London; University College London
  摘要：Genomic sequence data harbor valuable information concerning the history of species divergence and interspecific gene flow and may offer important insights into conservation of endangered species. However, extracting such information from genomic data requires powerful statistical inference methods. A recent analysis of genomic sequence data found little evidence for gene flow from domestic Bactrian camels into the endangered wild Bactrian species. Nevertheless, the methods used to infer gene ...
• Active bacterial baths in droplets AI翻译
  作者：Villalobos-Concha, Cristian; Liu, Zhengyang; Ramos, Gabriel; Goral, Martyna; Lindner, Anke; Lopez-Leon, Teresa; Clement, Eric; Soto, Rodrigo; Cordero, Maria Luisa
  作者单位：Universidad de Chile; Centre National de la Recherche Scientifique (CNRS); Universite PSL; Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI); Sorbonne Universite; Universite Paris Cite; Universite PSL; Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI); Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); Universite de Toulouse; Universite Toulouse III - Paul Sabatier; Institut Universitaire de France
  摘要：Suspensions of self-propelled objects represent a novel paradigm in colloidal science. In such active baths, traditional concepts such as Brownian motion, fluctuation-dissipation relations, and work extraction from heat reservoirs, must be extended beyond the conventional framework of thermal baths. Unlike thermal baths, which are characterized by a single parameter, the temperature, the fundamental descriptors of an active bath remain elusive. Particularly relevant are confined environments, ...
• A granulin-positive macrophage subtype in mycobacterial granulomas alleviates tissue damage by limiting excessive inflammation AI翻译
  作者：Luo, Geyang; Wen, Yanling; Wang, Min; Wang, Hao; Li, Duoduo; Liao, Mingfeng; Zeng, Dong; Luo, Sizhu; Niu, Liangfei; Sun, Tao; Sun, Peng; Qin, Liyi; Li, Weimin; Song, Shu; Takiff, Howard E.; Zhang, Shuye; Gao, Qian; Zhang, Zheng; Yan, Bo
  作者单位：Fudan University; Southern University of Science & Technology; Fudan University; Fudan University; Capital Medical University; Venezuelan Institute Science Research; Fudan University; The Third People's Hospital of Shenzhen
  摘要：Granulomas play a crucial role in the pathology of tuberculosis, but the immune environment governing their formation remains largely unknown. To explore the dynamic changes in the immune microenvironment during the formation of tuberculous granulomas, we infected adult zebrafish with Mycobacterium marinum and then examined uninfected and infected kidneys, as well as large and small granulomas in the kidneys. Using single-cell RNA sequencing technology, we identified two major macrophage subpo...
• SARS- CoV-2 uptake and inflammatory response in senescent endothelial cells are regulated by the BSG/VEGFR2 pathway AI翻译
  作者：Sakurai, Yuya; Fujioka, Yoichiro; Maishi, Nako; Takeda, Ryo; Ohba, Yusuke; Sasaki, Michihito; Teshirogi, Takahito; Ito, Wataru; Hida, Yasuhiro; Matsuda, Aya; Kido, Kanta; Orba, Yasuko; Sawa, Hirofumi; Hida, Kyoko
  作者单位：Hokkaido University; Hokkaido University; Hokkaido University; Hokkaido University; Hokkaido University; Hokkaido University; Hokkaido University; Hokkaido University; Fujita Health University; Fujita Health University; Hokkaido University
  摘要：Aging is a risk factor for severe COVID-19, characterized by vascular endothelial dysfunction. Although possible susceptibility of vascular endothelial cells (ECs) to SARS-CoV-2 infection has been suggested, the details of entry into cells have not been clarified. Previously, we reported that in an aged mouse model of severe COVID-19, ECs show a massive viral uptake and inflammatory response. Here, we focused on the endocytic capacity of senescent ECs. We found that the senescent ECs showed hi...
• Nonsubstrate PI(4,5)P2 interacts with the interdomain linker to control electrochemical coupling in voltage-sensing phosphatase (VSP) AI翻译
  作者：Mizutani, Natsuki; Yonezawa, Yasushige; Nakagawa, Atsushi; Okamura, Yasushi
  作者单位：University of Osaka; University of Osaka; Japan Society for the Promotion of Science; University of Osaka; Kindai University (Kinki University); University of Osaka; Jichi Medical University
  摘要：Voltage-sensing phosphatase (VSP) comprises a voltage sensor domain (VSD) and a cytoplasmic catalytic region (CCR), achieving a unique electrochemical signal conversion. Previous studies suggest that phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), a membrane phospholipid known to be critical for activities of diverse voltage-gated ion channels, associates with a linker connecting the VSD with the CCR of VSP and regulates VSD-CCR coupling. However, the details of PI(4,5)P2 interaction with t...
• Ultradian rhythms of CRHPVN neuron activity, behavior, and stress hormone secretion AI翻译
  作者：Zheng, Shaojie; Focke, Caroline M. B.; Young, Calvin K.; Tripp, Isaac; Ganeshan, Dharshini; Power, Emmet M.; Schwenke, Daryl O.; Herbison, Allan E.; Kim, Joon S.; Iremonger, Karl J.
  作者单位：University of Otago; University of Otago; University of Cambridge
  摘要：The stress axis is always active, even in the absence of any threat. This manifests as hourly pulses of corticosteroid stress hormone secretion over the day. Corticotropin- releasing control both the neuroendocrine stress axis as well as stress- associated behaviors. However, it is currently unclear how the resting activity of these neurons is coordinated with both spontaneous behavior and ultradian pulses of corticosteroid secretion. To ity in Crh- Ires- Cre mice and a newly generated line of...
• From biologging to conservation: Tracking individual performance in changing environments AI翻译
  作者：Ellis-Soto, Diego; Flack, Andrea; Strandburg-Peshkine, Ariana; Wild, Timm A.; Williams, Hannah J.; O'Mara, M. Teague
  作者单位：University of California System; University of California Berkeley; Yale University; Max Planck Society; Max Planck Society; University of Konstanz; Max Planck Society; University of Konstanz; University of Louisiana System; Southeastern Louisiana University; Smithsonian Institution; Smithsonian Tropical Research Institute
  摘要：Under an accelerating biodiversity crisis, increased urbanization, habitat fragmentation, and climate change require new approaches to assess conservation impact. We argue that animal biologging is a cost-effective method for monitoring biodiversity at its source, including tracked animals and the habitats they occupy. Biologging, or animal-mounted sensors to record data, can act as a reporting, measurement, and verification system, and deliver direct insights into environments of selection. U...