31期

最新文章

• Combining MicroED and native mass spectrometry for structural discovery of enzyme-small molecule complexes AI翻译
  作者：Vlahakis, Niko W.; Flowers, Cameron W.; Liu, Mengting; Agdanowski, Matthew P.; Johnson, Samuel; Summers, Jacob A.; Jacobs, Lian M. C.; Keyser, Catherine; Russell, Phoebe; Rose, Samuel L.; Orlans, Julien; Adhami, Nima; Chen, Yu; Sawaya, Michael R.; Basu, Shibom; de Sanctis, Daniele; Chen, Yu; Wakatsuki, Soichi; Nelson, Hosea M.; Loo, Joseph A.; Tang, Yi; Rodriguez, Jose A.
  作者单位：University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; National Science Foundation (NSF); NSF - Science and Technology Centers (STCs); University of California System; University of California Los Angeles; University of California System; University of California Los Angeles; California Institute of Technology; Stanford University; Stanford University; United States Department of Energy (DOE); SLAC National Accelerator Laboratory; State University System of Florida; University of South Florida; European Molecular Biology Laboratory (EMBL)
  摘要：With the goal of accelerating the discovery of small molecule-protein complexes, we leverage fast, low-dose, event-based electron counting microcrystal electron diffraction (MicroED) data collection and native mass spectrometry. This approach, which we term electron diffraction with native mass spectrometry (ED-MS), allows assignment of protein target structures bound to ligands with data obtained from crystal slurries soaked with mixtures of known inhibitors and crude biosynthetic reactions. ...
• Absence of phonon softening across a charge density wave transition due to quantum fluctuations AI翻译
  作者：Chen, Yubi; Kongruengkit, Terawit; Salinas, Andrea Capa; Yang, Runqing; Quan, Yujie; Zhang, Fanghao; Pokharel, Ganesh; Kautzsch, Linus; Wilson, Stephen D.; Mu, Sai; Harter, John W.; Liao, Bolin
  作者单位：University of California System; University of California Santa Barbara; University of California System; University of California Santa Barbara; University of California System; University of California Santa Barbara; University System of Georgia; University of West Georgia; University of South Carolina System; University of South Carolina Columbia
  摘要：Kagome metals have emerged as a frontier in condensed matter physics due to their potential to host exotic quantum states. Among these, CsV3Sb5 has attracted significant attention for the unusual coexistence of charge density wave (CDW) order and unconventional superconductivity, presenting an ideal system for exploring the emergent phenomena from the interplay of phonons, electronic fluctuations, and topological effects. The nature of CDW formation in CsV3Sb5 is unconventional and has sparked...
• Semantic change in adults is not primarily a generational phenomenon AI翻译
  作者：Kamath, Gaurav; Yang, Michelle; Reddy, Siva; Sonderegger, Morgan; Card, Dallas
  作者单位：McGill University; Mila Quebec Artificial Intelligence Institute; McGill University; University of Michigan System; University of Michigan
  摘要：A central question in the study of language change is whether or not such change is generational. If a language changes over time generation-by-generation, the process looks as follows: New generations of speakers introduce innovations, while older speakers conserve their usage patterns, and the language changes as new generations replace older ones. At the opposite extreme, language change could be a zeitgeist phenomenon, in which changes are universally adopted by speakers simultaneously, re...
• Generation of actionable, cancer-specific neoantigens from KRAS(G12C) with adagrasib AI翻译
  作者：Maso, Lorenzo; Rajak, Epsa; Hattori, Takamitsu; Hu, Zhengshan; Koide, Akiko; Neel, Benjamin G.; Koide, Shohei
  作者单位：New York University; New York University; New York University
  摘要：Effective immune therapy against cancer ideally should target a cancer-specific antigen, an antigen that is present exclusively in cancer cells. However, there is a paucity of cancer-specific antigens that are endogenously produced. HapImmuneTM technology utilizes covalent inhibitors directed to an intracellular cancer driver to create cancer-specific neoantigens in the form of drug-peptide conjugates presented by class I MHC molecules. Our previous study with sotorasib, an FDA-approved covale...
• Fossil samples archive functional diversity in marine ecosystems: An empirical test from a present-day coastal environment AI翻译
  作者：Tyler, Carrie L.; Kowalewski, Micha
  作者单位：Nevada System of Higher Education (NSHE); University of Nevada Las Vegas; State University System of Florida; University of Florida
  摘要：The fossil record of functional diversity is increasingly used to study ecosystem evolution, extinction recovery, and factors affecting long-term trends in biodiversity. In addition, the youngest fossil record (late Quaternary) can provide insights into the natural range of functional variability of present-day ecosystems, providing a historical framework for conservation and restoration. However, the reliability of common functional diversity measures derived from fossils is uncertain. If fos...
• Synergies between speciation and conservation science yield novel insights for mitigating the biodiversity crisis of the Anthropocene AI翻译
  作者：Wang, Silu; Yoder, Anne D.
  作者单位：State University of New York (SUNY) System; University at Buffalo, SUNY; Duke University
• DICER-LIKE 5 loss causes thermosensitive male sterility in durum wheat and reveals an AU-rich motif guiding 24-nt phasiRNA biogenesis AI翻译
  作者：Belanger, Sebastien; Martin, Azahara C.; Marchant, D. Blaine; Zhan, Junpeng; McGregor, Madison; Smedley, Mark; Hayta, Sadiye; Moore, Graham; Meyers, Blake C.
  作者单位：Donald Danforth Plant Science Center; James Hutton Institute; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); John Innes Center; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Instituto de Agricultura Sostenible (IAS); University of Missouri System; University of Missouri Saint Louis; Huazhong Agricultural University; Huazhong Agricultural University; Hubei Hongshan Laboratory; University of California System; University of California Davis; University of California System; University of California Davis
  摘要：Reproductive, male-enriched small RNAs are present in flowering plants and animals, yet their role in plants remains underexplored. We generated dicer-like 5 (dcl5) mutants in durum wheat (Triticum turgidum ssp. durum 2n = 4x = 28; AABB), revealing temperature-sensitive genic male sterility. Loss of DCL5 depleted premeiotic and meiotic 24-nt phasiRNA production, correlating with sterility under standard growth conditions and partial fertility recovery at higher temperatures. A single functiona...
• Foraging ants as liquid brains: Movement heterogeneity shapes collective efficiency AI翻译
  作者：Fernandez-Lopez, Pol; Oro, Daniel; Lloret-Cabot, Roger; Genovart, Meritxell; Garriga, Joan; Bartumeus, Frederic
  作者单位：Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Centre d'Estudis Avancats de Blanes (CEAB); Universite de Toulouse; Universite Toulouse III - Paul Sabatier; Centre National de la Recherche Scientifique (CNRS); ICREA; Centro de Investigacion Ecologica y Aplicaciones Forestales (CREAF-CERCA)
  摘要：Liquid brains conceptualize living systems that operate without central control, where collective outcomes emerge from local and dynamic interactions. This concept extends beyond ants and other social insects to include immune systems, slime molds, and microbiomes. In such systems, connectivity scales with population density, facilitating more efficient information transfer as group size increases. However, in sparse conditions, where fewer individuals interact, movement likely plays a crucial...
• Surface delivery quantification reveals distinct trafficking efficiencies among clustered protocadherin isoforms AI翻译
  作者：May, Elizabeth J.; Gaudet, Rachelle
  作者单位：Harvard University; University of California System; University of California Berkeley
  摘要：Proteins that transmit molecules and signals across the plasma membrane are crucial in cell biology because they enable cells to sense and respond to their surroundings. A major challenge for studying cell surface proteins is that often they do not fold or traffic properly to the plasma membrane when produced in heterologous cells. We developed a strategy for quantifying surface localization from fluorescence microscopy images of surface-stained cells. Using clustered protocadherins, a protein...
• Rare variants in BMAL1 are associated with a neurodevelopmental syndrome AI翻译
  作者：Cuddapah, Vishnu Anand; Chen, Dechun; Cho, Bumsik; Moore, Rebecca; Suri, Mohnish; Safraou, Hana; Tran-Mau-Them, Frederic; Wilson, Ashley; Odgis, Jacqueline; Rehman, Atteeq U.; Saunders, Carol; Ganesan, Shiva; Jobanputra, Vaidehi; Scherer, Stephen W.; Helbig, Ingo; Sehgal, Amita
  作者单位：Baylor College of Medicine; Baylor College Medical Hospital; Baylor College of Medicine; Howard Hughes Medical Institute; University of Pennsylvania; University of Pennsylvania; Nottingham University Hospital NHS Trust; Universite Bourgogne Europe; CHU Dijon Bourgogne; Institut National de la Sante et de la Recherche Medicale (Inserm); Universite Bourgogne Europe; Icahn School of Medicine at Mount Sinai; University of Missouri System; University of Missouri Kansas City; Children's Mercy Hospital; University of Missouri System; University of Missouri Kansas City; University of Pennsylvania; Pennsylvania Medicine; Childrens Hospital of Philadelphia; NewYork-Presbyterian Hospital; Columbia University; University of Toronto; Hospital for Sick Children (SickKids); University of Toronto; University of Toronto; University of Pennsylvania
  摘要：Through international gene- matching efforts, we identified 10 individuals with ultrarare heterozygous variants, including 5 de novo variants, in BMAL1, a core component of the molecular clock. Instead of an isolated circadian phenotype seen with disease- causing variants in other molecular clock genes, all individuals carrying BMAL1 variants surprisingly share a clinical syndrome manifest as developmental delay and autism spectrum disorder, with variably penetrant sleep disturbances, seizures...