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作者：Io, Skyler Wu; Meyer, Austin G.; Clemente, Leonardo; Stolerman, Lucas M.; Lu, Fred; Majumder, Atreyee; Verbeeck, Rudi; Masyn, Serge; Santillana, Mauricio
作者单位：Harvard University; Northeastern University; Baylor College of Medicine; Oklahoma State University System; Oklahoma State University - Stillwater; Harvard University; Harvard T.H. Chan School of Public Health
摘要：Dengue fever, a tropical vector-borne disease, is a leading cause of hospitalization and death in many parts of the world, especially in Asia and Latin America. Where timely dengue surveillance exists, decision-makers can better implement public health measures and allocate resources. Reliable near-term forecasts may help anticipate healthcare demands and promote preparedness. We propose ensemble modeling approaches combining mechanistic, statistical, and machine learning models to forecast de...
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作者：Elboim, Dor; Peres, Yuval; Peretz, Ron
作者单位：Stanford University; Bar Ilan University
摘要：In several settings (e.g., sensor networks and social networks), nodes of a graph are equipped with initial opinions, and the goal is to estimate the average of these opinions using local operations. A natural algorithm to achieve this is the edge-averaging process, where edges are repeatedly selected at random (according to independent Poisson clocks) and the opinions on the nodes of each selected edge are replaced by their average. The effectiveness of this algorithm is determined by its con...
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作者：Sugo, Noriyuki; Uchimura, Arikuni; Matsumoto, Risa; Nakayama, Hiro; Fujimoto, Shota; Mizuno, Saya; Higuchi, Mayumi; Toshishige, Masaaki; Satoh, Yasunari; Wakayama, Sayaka; Wakayama, Teruhiko; Yagi, Takeshi
作者单位：University of Osaka; Radiation Effects Research Foundation - Japan; University of Yamanashi
摘要：Somatic mutations in cortical neurons have been implicated in psychiatric disorders. While endogenous DNA damage and repair errors are potential contributors to these mutations during development, the underlying mutagenic mechanism remains unclear. Here, we investigated somatic mutations in immature cortical neurons using mouse somatic cell nuclear transfer-derived embryonic stem cells and whole-genome sequencing. Insertions and deletions (indels) were commonly observed in both repeat and nonr...
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作者：Brecier, Aurelie; Mailhos, Gaelle; Jarzebowski, Przemyslaw; Li, Yuqi; Paulsen, Ole; Hay, Y. Audrey
作者单位：Universite Jean Monnet; Universite Lyon 1; Centre National de la Recherche Scientifique (CNRS); Institut National de la Sante et de la Recherche Medicale (Inserm); CNRS - National Institute for Biology (INSB); University of Cambridge; University of London; University College London
摘要：Non-rapid eye movement (NREM) sleep facilitates memory consolidation by transferring information from the hippocampus to the neocortex. This transfer is thought to occur primarily when hippocampal sharp-wave ripples (SWRs) and thalamocortical spindles are synchronized. However, the mechanisms underlying this synchronization remain unknown. In this study, we investigated the role of cortical layer 1 neuron-derived neurotrophic factor (NDNF)-expressing (L1 NDNF) interneurons in gating informatio...
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作者：Pallavi, Anuma; Sinha, Neha; ArunKumar, Nagapriya K.; Kothegala, Lakshmi; Gandasi, Nikhil R.
作者单位：Indian Institute of Science (IISC) - Bangalore; University of Gothenburg; Uppsala University
摘要：Glucose transporters (GLUT1/2) facilitate glucose uptake in pancreatic beta cells, triggering insulin secretion. The availability of GLUTs at the plasma membrane (PM) is governed by its expression, delivery to the membrane, and endocytosis. Little is known about the dynamics of GLUT trafficking in response to glucose that triggers glucose-stimulated insulin secretion. In our study, we found that the recruitment of GLUTs to the PM of beta cells correlates with increasing glucose concentrations....
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作者：Frail, Sarah; Steele-Ogus, Melissa; Doenier, Jon; Moulin, Solene L. Y.; Braukmann, Tom; Xu, Shouling; Yeh, Ellen
作者单位：Stanford University; Stanford Medicine; Stanford University; Stanford Medicine; Carnegie Institution for Science; Stanford Medicine; Stanford University; Chan Zuckerberg Initiative (CZI)
摘要：Endosymbiotic gene transfer (EGT) and import of host-encoded proteins have been considered hallmarks of organelles necessary for stable integration of two cells. However, newer endosymbiotic models have challenged the origin and timing of such genetic integration during organellogenesis. Epithemia diatoms contain diazoplasts, obligate endosymbionts derived from cyanobacteria that are closely phylogenetically related to UCYN-A, a recently described nitrogen-fixing organelle. Diazoplasts functio...
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作者：Ogo, Yuko; Kawauchi, Takumi; Mimura, Manaki; Naito, Ken; Itoh, Hironori; Izawa, Takeshi
作者单位：National Agriculture & Food Research Organization - Japan; University of Tokyo; National Agriculture & Food Research Organization - Japan; National Agriculture & Food Research Organization - Japan
摘要：The Ghd7 (Grain number, plant height, and heading date 7)gene integrates red light signals and circadian rhythms to control floral repression under long-day conditions in rice. CRISPR/Cas9 systems were employed to create a series of deletion mutant lines in the upstream regions of Ghd7, covering a 65-kb genomic region from its transcription start site (TSS). These deletions ranged from 2 to 25 kb in size. Three deletion lines, those from 0 to-3 kb (0/-3 K), -20 to-40 kb (-20/-40 K), and-26 to-...
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作者：Bellucci, Michael A.; Amiri, Mehdi; Berryman, Stephen; Moshari, Andia; Owino, Collins Oduor; Luteijn, Rutger D.; Tuthill, Tobias J.; Svitkin, Yuri; Belsham, Graham J.; van Kuppeveld, Frank J. M.; Sonenberg, Nahum
作者单位：McGill University; McGill University; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); Pirbright Institute; Utrecht University; University of Copenhagen
摘要：Viruses have evolved elaborate mechanisms to hijack the host mRNA translation machinery to direct viral protein synthesis. Picornaviruses, whose RNA genome lacks a cap structure, inhibit cap-dependent mRNA translation, and utilize an internal ribosome entry site (IRES) in the RNA 5 ' untranslated region to recruit the 40S ribosomal subunit. IRES activity is stimulated by a set of host proteins termed IRES trans-acting factors (ITAFs). The cellular protein ITAF45 (also known as PA2G4 or EBP1) w...
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作者：Lin, Xiaohe; Cottrell, Christopher A.; Kalyuzhniy, Oleksandr; Tingle, Ryan; Kubitz, Michael; Lu, Danny; Yuan, Meng; Schief, William R.; Wilson, Ian A.
作者单位：Scripps Research Institute; Scripps Research Institute; Scripps Research Institute; Scripps Research Institute
摘要：The development of germline-targeting vaccines represents a potentially transformative strategy to elicit broadly neutralizing antibodies (bnAbs) against HIV and other antigenically diverse pathogens. Here, we report on structural characterization of vaccine-elicited VRC01-class bnAb precursors in the IAVI G001 Phase 1 clinical trial with the eOD-GT8 60mer nanoparticle as immunogen. High-resolution X-ray structures of eOD-GT8 monomer complexed with Fabs of five VRC01-class bnAb precursors with...
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作者：Ota, Naruhisa; Davies, Christopher W.; Kang, Jing; Yan, Donghong; Scherl, Alexis; Wong, Anne; Cook, Ryan; Tao, Xun; Dunlap, Debra; Klabunde, Sha; Mantik, Priscilla; Mohanan, Vishnu; Lin, WeiYu; McBride, Jacqueline; Sadekar, Shraddha; Storek, Kelly M.; Lupardus, Patrick; Ye, Zhengmao; Wallweber, Heidi Ackerly; Kiefer, James R.; Xu, Min; Chan, Pamela; Nagapudi, Karthik; Yi, Tangsheng; Koerber, James T.
