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作者：Gkragkopoulou, Polyniki; Garrett, Stephen R.; Shah, Prakhar Y.; Grebenc, Dirk W.; Klein, Timothy A.; Kim, Youngchang; Whitney, John C.
作者单位：McMaster University; McMaster University; United States Department of Energy (DOE); Argonne National Laboratory; McMaster University
摘要：To survive in highly competitive environments, bacteria use specialized secretion systems to deliver antibacterial toxins into neighboring cells, thereby inhibiting their growth. In many Gram-positive bacteria, the export of such toxins requires a membrane-bound molecular apparatus known as the type VIIb secretion system (T7SSb). Recently, it was shown that toxin recruitment to the T7SSb requires a physical interaction between a toxin and two or more so-called targeting factors, which harbor k...
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作者：Hagerman, Paul J.
作者单位：University of California System; University of California Davis
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作者：Kubatova, Nina; Schmidt, Thomas; Wang, Quan; Clore, G. Marius
作者单位：National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
摘要：G protein-coupled receptors (GPCRs) comprise a large class of membrane proteins that mediate cellular responses to a wide range of external signals and as such constitute major drug targets. While oligomerization has been shown to play a well-established role in modulating signaling for class C GPCRs (e.g., the glutamate and GABA receptors), the functional relevance of oligomerization for class A receptors, such as the beta(1)-adrenergic receptor (beta(1)AR), remains unclear. Here, we have exa...
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作者：Zhou, Yu; Wei, Chen; Jin, Lihua
作者单位：University of California System; University of California Los Angeles
摘要：Liquid crystal elastomers (LCEs) are anisotropic, viscoelastic materials integrating polymer networks and liquid crystals. While their mechanical responses have been extensively studied, their fracture behavior remains largely unexplored. Specifically, the effect of the deformation-director coupling on LCE fracture paths is unknown, and fracture criteria for LCEs are not yet established. To address this gap, we combine experimental and theoretical approaches to investigate fracture propagation...
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作者：Brott, Barbara K.; Raissi, Aram J.; Micheva, Kristina D.; Vielmetter, Jost; Mendes, Monique S.; Baccus, Caroline J.; Huang, Jolie; Shatz, Carla J.
作者单位：Stanford University; Stanford University; Stanford University; California Institute of Technology
摘要：Synapse pruning sculpts neural circuits throughout life. The human Leukocyte immunoglobulin-like receptor type B2 (LilrB2)/murine Paired immunoglobulin receptor B (PirB) receptors expressed in neurons and complement protein C4 have been separately implicated in pruning. Here, we report that C4d, a C4 cleavage product with unknown function, binds LilrB2/PirB with nanomolar affinity. C4d and LilrB2 colocalize at excitatory synapses in the human cerebral cortex as well as with beta amyloid in Alz...
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作者：Mausz, Michaela A.; Murphy, Andrew R. J.; Ferretjans, Maria del Mar Aguilo-; Hitchcock, Andrew; Moran, Mary Ann; Scanlan, David J.; Chen, Yin; Lidbury, Ian D. E. A.
作者单位：University of Warwick; University of Sheffield; University of Sheffield; University System of Georgia; University of Georgia; University of Birmingham
摘要：Across all domains of life, cobalamin-dependent methyltransferases have diversified to perform a range of crucial functions, such as methionine synthesis and the demethylation of various reduced nitrogen and sulfur compounds. These large modular enzymes typically possess three substrate-binding domains, two binding either the methyl donor or methyl acceptor, as well as a cobalamin-binding domain. Here, by challenging the current paradigm of glycine betaine (GBT) catabolism, we have identified ...
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作者：Hong, Hu; Wei, Zhiquan; Wang, Yiqiao; Yang, Xinru; Guo, Xun; Nian, Qingshun; Li, Xinliang; Li, Qing; Wang, Shixun; Li, Shimei; Zhang, Dechao; Xiong, Qi; Huang, Zhaodong; Zhi, Chunyi
作者单位：City University of Hong Kong; The University of Hong Kong Shenzhen Institute of Research & Innovation; University of Hong Kong; University of Hong Kong; Zhengzhou University; University of Macau; University of Hong Kong; University of Hong Kong
摘要：Solid-state electrolytes (SSEs) are being extensively researched as replacements for liquid electrolytes in future batteries. Despite significant advancements, there are still challenges in using SSEs, particularly in extreme conditions. This study presents a hydrated metal-organic ionic cocrystal (HMIC) solid-state ion conductor with a solvent-assisted ion transport mechanism suitable for extreme operating conditions. Through crystal engineering strategies, the adsorption capacity of HMIC for...
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作者：McKay, Conor E.; Deans, Miles; Connor, Jack; Saunders, Janet C.; Lloyd, Christopher; Radford, Sheena E.; Brockwell, David J.
作者单位：University of Leeds; University of Leeds; AstraZeneca
摘要：Deep mutational scanning (DMS) assays provide a powerful method to generate large-scale datasets essential for advancing AI-driven predictions in biology. The tripartite beta- lactamase assay (TPBLA), in which a protein of interest is inserted between two domains of beta- lactamase, has previously been reported as capable of detecting and quantitating the aggregation of proteins and biologics in the oxidizing periplasm of Escherichia coli and used as a platform for identifying small molecule i...
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作者：Sefik, Esen; Philbrick, William; Zhang, Fengrui; Agrawal, Kriti; Van Lee, Brian; Sam, Johannes; Karatepe, Kutay; Zheng, Yunjiang; Liang, Kaixin; Peng, Sophia; Mirza, Haris; Rangavajhula, Athreya; Simon, Perrine; Arun, Neha; Babu, Priyanka; Eynon, Elizabeth; Chiorazzi, Michael; Shan, Liang; Halene, Stephanie; Luo, Hongbo R.; Rongvaux, Anthony; Kluger, Yuval; Flavell, Richard A.
作者单位：Yale University; Yale University; Yale University; Roche Holding; Yale University; Yale University; Yale University; Saint Louis University; Washington University (WUSTL); Yale University; Harvard University; Harvard University Medical Affiliates; Brigham & Women's Hospital; Fred Hutchinson Cancer Center; Yale University; Howard Hughes Medical Institute; Yale University
摘要：Murine and human immune systems differ significantly, particularly within the myeloid lineage. Humanized mice, generated by transplanting human hematopoietic stem, progenitor cells into genetically modified mice, are invaluable to study human immune development and function in vivo. However, a major limitation of current models is suboptimal myelopoiesis, particularly lack of functional human neutrophils, hampering the modeling of human immune responses and chronic diseases. Here, we describe ...
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作者：Tobias, Joseph A.; Bullock, James M.; Dicks, Lynn V.; Forester, Brenna R.; Razgour, Orly
