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作者：Michaowski, Michal A.; Terejko, Katarzyna; Gos, Michalina; Izykowska, Ilona; Czyzewska, Marta M.; Kopotowski, Karol; Kaczor, Przemysaw T.; Brzostowicz, Aleksandra; Puzek, Estera; Migdaek, Monika; Mozrzymas, Jerzy W.
作者单位：Wroclaw Medical University; University of Wroclaw; Wroclaw University of Environmental & Life Sciences; Polish Academy of Sciences; Hirszfeld Institute of Immunology & Experimental Therapy of the Polish Academy of Sciences
摘要：gamma- aminobutyric acid type A receptor (GABAAR) is a pentameric ligand-gated ion channel that plays a crucial role in inhibition in the adult brain. Structural and electrophysiological studies have provided numerous insights into the receptor's functioning but the complete molecular mechanism of GABAAR action remains elusive. Herein, we used high-resolution single-channel recording to analyze point-mutated alpha 1 beta 2 gamma 2 receptors and applied so called Phi value (REFER, rate-equilibr...
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作者：Junker, Robert R.; Farwig, Nina
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作者：Kim, David E.; Watson, Joseph L.; Juergens, David; Majumder, Sagardip; Sonigra, Ria; Gerben, Stacey R.; Kang, Alex; Bera, Asim K.; Li, Xinting; Baker, David
作者单位：University of Washington; University of Washington Seattle; University of Washington; University of Washington Seattle; Howard Hughes Medical Institute; University of Washington; University of Washington Seattle
摘要：Francis Crick's global parameterization of coiled coil geometry has been widely useful for guiding design of new protein structures and functions. However, design guided by similar global parameterization of beta barrel structures has been less successful, likely due to the deviations from ideal barrel geometry required to maintain inter-strand hydrogen bonding without introducing backbone strain. Instead, beta barrels have been designed using two-dimensional structural blueprints; while this ...
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作者：Mitani, Takahiro; Takeda, Shuichi; Oda, Toshiro; Narita, Akihiro; Maeda, Yuichiro; Honda, Hajime; Fujiwara, Ikuko
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作者：Slaughter, Isaac; Peytavin, Axel; Ugander, Johan; Saveski, Martin
作者单位：University of Washington; University of Washington Seattle; Stanford University; Yale University
摘要：Social networks scaffold the diffusion of information on social media. Much attention has been given to the spread of true vs. false content on online social platforms, including the structural differences between their diffusion patterns. However, much less is known about how platform interventions on false content alter the engagement with and diffusion of such content. In this work, we estimate the causal effects of Community Notes, a novel fact-checking feature adopted by X (formerly Twitt...
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作者：Chen, Xiangjun; Hwang, Hee Seung; Li, Bihua; Zhao, Yanhua; Ghosh, Koushik; Deng, Lei; Creasey, Elizabeth A.; Ashenberg, Orr; Graham, Daniel B.; Xavier, Ramnik J.
作者单位：Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Massachusetts Institute of Technology (MIT); Broad Institute; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Brigham & Women's Hospital; Harvard University; Harvard Medical School
摘要：Nuclear receptors (NRs) are crucial to integrate metabolite sensing and immune responses in the gut. NR- interacting protein 1 (NRIP1) is an important coregulator of various NRs that has been implicated in inflammatory bowel disease risk, but mechanistic details of how NRIP1 controls NR activities mediating immune homeostasis and inflammation remain elusive. We demonstrate that a missense risk tory cytokine production, ultimately leading to exacerbated intestinal inflammation. Mechanistically,...
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作者：Tewary, Aniruddha; Sengupta, Shreya; Mukherjee, Soumya; Hazra, Nilanjana; Ebright, Yon W.; Ebright, Richard H.; Mukhopadhyay, Jayanta
作者单位：Department of Science & Technology (India); Bose Institute; Rutgers University System; Rutgers University New Brunswick; Rutgers University System; Rutgers University New Brunswick; University System of Ohio; University of Toledo; University System of Ohio; University of Toledo
摘要：A 6 cycle in which the initiation factor 6 associates with RNA polymerase (RNAP) core enzyme to permit transcription initiation and dissociates from RNAP core enzyme to permit transcription elongation, has been proposed to occur and to be an essential step for 6-exchange, with all principal 6 factors from all bacteria. These proposals were based on studies of the principal 6 factor of Escherichia coli, 670, which generally, albeit not obligatorily, is released from RNAP upon the transition fro...
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作者：Weed, Lara; Zeitzer, Jamie M.
作者单位：Stanford University; Stanford University; Stanford Medicine
摘要：Seasonal changes in time policy, such as switching between Standard Time (SDT) and Daylight Saving Time (DST), have been adopted by many countries, including the United States. While transitioning between SDT and DST has notable acute negative population health impacts, the chronic impact of these time policies on health has not been well evaluated. To estimate the impact of permanent SDT or DST on health, we modeled the circadian impact of SDT, DST, and Biannual Shifting (BAS) across a year i...
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作者：Bentley, Kirsten; Statkute, Evelina; Murrell, Isa; Fielding, Ceri A.; Antrobus, Robin; Preston, Hannah; Jones, Lauren Kerr -; Cochrane, Daniel; Brizic, Ilija; Lehner, Paul J.; Wilkinson, Gavin W. G.; Wang, Eddie C. Y.; Graham, Stephen C.; Weekes, Michael P.; Stanton, Richard J.
作者单位：University of Cambridge; University of Rijeka; University of Cambridge; University of Cambridge
摘要：Human cytomegalovirus (HCMV) is a clinically important herpesvirus that has coevolved for millions of years with its human host, and establishes lifelong persistent infection. A substantial proportion of its 235 kb genome is dedicated to manipulating host immunity through targeting antiviral host proteins for degradation or relocalization. Quantitative proteomics of the infected cell has extensively characterized these processes, but the cell-free virion has been less well studied. We therefor...
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作者：Subramanian, Bhuvaneshwaran; Johnson, Shedreanna; Narayanan, Akshaya; Wang, Wei; Seaberg, Bonnie L.; Ball, Jillian; Mares, Ilse M. Paredes; Majumder, Ahana; Aceves, Alexandria; Frazier, Sarah E.; Rutledge, Alexis; Griffin, John F.; Pal, Soumiya; Zawieja, Scott; Davis, Michael J.; Rutkowski, Joseph M.; Nghiem, Peter P.; Rimer, Mendell; Muthuchamy, Mariappan
作者单位：Texas A&M University System; Texas A&M University College Station; Texas A&M University System; Texas A&M University College Station; Texas A&M University System; Texas A&M University College Station; University of Missouri System; University of Missouri Columbia
摘要：Duchenne muscular dystrophy (DMD) is a severe muscle-wasting disorder characterized by progressive muscle weakness and inflammation caused by mutations in the DMD gene. Chronic inflammation in DMD exacerbates the complications associated with disease progression. Since the lymphatic system plays a crucial role in regulating and resolving inflammation, our primary goal was to investigate whether lymphatics were dysregulated in skeletal muscle of DMD animals. We used the D2.mdx murine and golden...
