Proceedings of the National Academy of Sciences of the United States of America

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• Cryo-ET suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules AI翻译
  作者：Chakraborty, Saikat; Martinez-Sanchez, Antonio; Beck, Florian; Toro-Nahuelpan, Mauricio; Hwang, In-Young; Noh, Kyung-Min; Baumeister, Wolfgang; Mahamid, Julia
  作者单位：Max Planck Society; University of Gottingen; UNIVERSITY GOTTINGEN HOSPITAL; University of Gottingen; UNIVERSITY GOTTINGEN HOSPITAL; Max Planck Society; European Molecular Biology Laboratory (EMBL); European Molecular Biology Laboratory (EMBL); European Molecular Biology Laboratory (EMBL); University of Oxford; University of Murcia
  摘要：The functional architecture of the long- lived neuronal microtubule (MT) cytoskeleton is maintained by various MT- associated proteins (MAPs), most of which are known to bind to the MT outer surface. However, electron microscopy (EM) has long ago revealed the presence of particles inside the lumens of neuronal MTs, of yet unknown identity and function. Here, we use cryogenic electron tomography (cryo-ET) to analyze the three- dimensional (3D) organization and structures of MT lumenal particles...
• Microbes could convert industrial wastewater into ecofriendly products From the dairy industry to tech, microbial helpers may offer a sustainable approach to handling waste AI翻译
  作者：Beans, Carolyn
• Monofluorinated acetal electrolyte for high- performance lithium metal batteries AI翻译
  作者：Zhang, Elizabeth; Chen, Yuelang; Holoubek, John; Yu, Zhiao; Zhang, Wenbo; Lyu, Hao; Choi, Il Rok; Kim, Sang Cheol; Serrao, Chad; Cui, Yi; Bao, Zhenan
  作者单位：Stanford University; Stanford University; Stanford University; Stanford University; United States Department of Energy (DOE); SLAC National Accelerator Laboratory
  摘要：High degree of fluorination for ether electrolytes has resulted in improved cycling stability of lithium metal batteries due to stable solid electrolyte interphase (SEI) formation and good oxidative stability. However, the sluggish ion transport and environmental concerns of high fluorination degree drive the need to develop less fluorinated structures. Here, we depart from the traditional ether backbone and introduce bis(2- fluoroethoxy)methane (F2DEM), featuring monofluorination of the aceta...
• Quantifying the genetic origins of body plan scaling AI翻译
  作者：Galstyan, Vahe; ten Wolde, Pieter Rein
  作者单位：AMOLF
• Modular DNA origami-based electrochemical detection of DNA and proteins AI翻译
  作者：Jeon, Byoung-jin; Guareschi, Matteo M.; Stewart, Jaimie Marie; Wu, Emily; Gopinath, Ashwin; Arroyo-Curras, Netzahualcoyotl; Dauphin-Ducharme, Philippe; Plaxco, Kevin W.; Lukeman, Philip S.; Rothemund, Paul W. K.
  作者单位：California Institute of Technology; University of California System; University of California Los Angeles; Massachusetts Institute of Technology (MIT); Johns Hopkins University; University of Sherbrooke; University of California System; University of California Santa Barbara; St. John's University
  摘要：The diversity and heterogeneity of biomarkers has made the development of general methods for single- step quantification of analytes difficult. For individual biomarkers, electrochemical methods that detect a conformational change in an affinity binder upon analyte binding have shown promise. However, because the conformational change must operate within a nanometer- scale working distance, an entirely new sensor, with a unique conformational change, must be developed for each analyte. Here, ...
• Higher- order transient structures and the principle of dynamic connectivity in membrane signaling AI翻译
  作者：Zhang, Yuxi; Mackinnon, Roderick
  作者单位：Rockefeller University; Howard Hughes Medical Institute; Rockefeller University
  摘要：We examine the role of higher- order transient structures (HOTS) in M2R regulation of GIRK channels. Electron microscopic membrane protein location maps show that both proteins form HOTS that exhibit a statistical bias to be near each other. Theoretical calculations and electrophysiological measurements suggest that channel activity is isolated near larger M2R HOTS. By invoking weak interactions that permit transient binding of M2R to M2R and GIRK to GIRK ( i-i interactions) and M2R to GIRK ( ...
• Language models reveal a complex sequence basis for adaptive convergent evolution of protein functions AI翻译
  作者：Cao, Zhenqiu; Zhang, Hongjiu; Zou, Zhengting
  作者单位：Chinese Academy of Sciences; Institute of Zoology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS
  摘要：Convergent evolution, or convergence, refers to repeated, independent emergences of the same trait in two or more lineages of species during evolution, often indicating functional adaptation to specific environmental factors. Many computational methods have been proposed to investigate the genetic basis for organismal functional convergence, as an important way to decode the complex sequence-function map of proteins. These methods mostly focus on the convergence of amino acid states at the lev...
• Evolution under vancomycin selection drives divergent collateral sensitivity patterns in Staphylococcus aureus AI翻译
  作者：Card, Kyle J.; Crozier, Dena; Durmaz, Arda; Gray, Jason; Creary, Justin; Stocks, Amira; Maltas, Jeff; Bonomo, Robert A.; Burke, Zachary D. C.; Scott, Jacob G.
  作者单位：Cleveland Clinic Foundation; Cleveland Clinic Foundation; University System of Ohio; Case Western Reserve University; Washington University (WUSTL); University System of Ohio; Case Western Reserve University; University System of Ohio; Case Western Reserve University; Cleveland Clinic Foundation
  摘要：Staphylococcus aureus bacteremia is typically treated empirically with vancomycin, with therapy later tailored based on susceptibility results. However, these tests occur before vancomycin exposure and do not account for adaptation during empiric treatment that can alter S. aureus' susceptibility to first-line drugs. To investigate these collateral drug responses, we experimentally evolved 18 methicillin-susceptible S. aureus (MSSA) populations under increasing vancomycin concentrations until ...
• A widespread family of ribosomal peptide metallophores involved in bacterial adaptation to metal stress (vol 121, e2408304121, 2024) AI翻译
  作者：Leprevost, Laura; Junge, Sophie; Lippens, Guy; Guillaume, Celine; Sicoli, Giuseppe; Oliveira, Lydie; Falcone, Enrico; de Santis, Emiliano; Rivera-Millot, Alex; Billon, Gabriel; Stellato, Francesco; Henry, Celine; Antoine, Rudy; Zirah, Severine; Dubiley, Svetlana; Li, Yanyan; Jacob-Dubuisson, Francoise
• Optimal transitions between nonequilibrium steady states AI翻译
  作者：Monter, Samuel; Loos, Sarah A. M.; Bechinger, Clemens
  作者单位：University of Konstanz; University of Cambridge; Max Planck Society
  摘要：The optimal control of finite-time processes on the microscale is of significant theoretical and practical interest, particularly for the energy-efficient operation of nanomachines. While previous studies have primarily focused on transitions between equilibrium states, many biologically and technologically relevant processes occur far from equilibrium. In such nonequilibrium settings, memory, a ubiquitous feature in realistic systems, plays an intricate role, as any driving necessarily excite...