Proceedings of the National Academy of Sciences of the United States of America

最新文章

• Measuring the selective packaging of RNA molecules by viral coat proteins in cells AI翻译
  作者：Rastandeh, Amineh; Makasarashvili, Nino; Dhaliwal, Herman K.; Baker, Sherry; Subramanian, Sundharraman; Villarreal, Daniel A.; Gamez, Elmer I.; Parent, Kristin N.; Garmann, Rees F.
  作者单位：California State University System; San Diego State University; California State University System; San Diego State University; Michigan State University; California State University System; San Diego State University
  摘要：Some RNA viruses package their genomes with extraordinary selectivity, assembling protein capsids around their own viral RNA while excluding nearly all host RNA. How the assembling proteins distinguish viral RNA from host RNA is not fully understood, but RNA structure is thought to play a key role. To test this idea, we perform in-cellulo packaging experiments using bacteriophage MS2 coat proteins and a variety of RNA molecules in Escherichia coli. In each experiment, plasmid-derived RNA molec...
• Detecting chirality-induced spin selectivity in chromophore-linked DNA hairpins using photogenerated radical pairs AI翻译
  作者：Latawiec, Elisabeth I.; Chiesa, Alessandro; Qiu, Yunfan; Tcyrulnikov, Nikolai A.; Young, Ryan M.; Carretta, Stefano; Krzyaniak, Matthew D.; Wasielewski, Michael R.
  作者单位：Northwestern University; Northwestern University; University of Parma; University of Parma; Consiglio Nazionale delle Ricerche (CNR); Istituto di Scienze e Tecnologie Molecolari (ISTM-CNR); Istituto Nazionale di Fisica Nucleare (INFN)
  摘要：Chirality-induced spin selectivity (CISS) results in spin polarization of electrons transmitted through chiral molecules and materials. Since CISS results in spin polarization even at room temperature, it affords the possibility of using it to develop quantum technologies that can operate under ambient conditions. We have shown previously that photo-driven hole transfer within DNA hairpins provides a facile route to generate spin-correlated radical pairs (SCRPs). To study the effect of CISS on...
• Regulation of CD45 isoforms during human effector and memory CD8 T cell differentiation: Implications for T cell nomenclature AI翻译
  作者：McGuire, Donald J.; Akondy, Rama S.; Yang, Shu; Edupuganti, Srilatha; Nagar, Shashi; Michael, Garett; Rosa, Stephen C. De; Newell, Evan W.; Farber, Donna L.; Kissick, Haydn T.; McElrath, M. Juliana; Ahmed, Rafi
  作者单位：Emory University; Emory University; Ashoka University; Tianjin Medical University; Tianjin Medical University; ICON plc; Fred Hutchinson Cancer Center; Columbia University; NewYork-Presbyterian Hospital; NewYork-Presbyterian Hospital; Columbia University; Emory University
  摘要：This study examines the expression of CD45 isoforms on human yellow fever virus vaccine (YFV-17D) specific CD8 T cells longitudinally after vaccination. As expected, effector CD8 T cells at day 14 express CD45RO but within 4 to 6 wk these virus-specific CD8 T cells become CD45RA positive and remain CD45RA for >10 y. The journey for these YFV-specific CD8 T cells goes from naive (CD45RA+ CCR7+) to effector/ effector memory (CD45RO+ CCR7-) to Temra (CD45RA+ CCR7-) to stem-cell memory (CD45RA+ CC...
• Computational investigation of water glasses using machine-learning potentials AI翻译
  作者：Szukalo, Ryan J.; Giovambattista, Nicolas; Debenedetti, Pablo G.
  作者单位：Princeton University; City University of New York (CUNY) System; Brooklyn College (CUNY); Princeton University
  摘要：The molecular origins of water's anomalous properties have long been a subject of scientific inquiry. The liquid-liquid phase transition hypothesis, which posits the existence of distinct low-density and high-density liquid states separated by a first-order phase transition terminating at a critical point, has gained increasing experimental and computational support and offers a thermodynamically consistent framework for many of water's anomalies. However, experimental challenges in avoiding c...
• From biologging to conservation: Tracking individual performance in changing environments AI翻译
  作者：Ellis-Soto, Diego; Flack, Andrea; Strandburg-Peshkine, Ariana; Wild, Timm A.; Williams, Hannah J.; O'Mara, M. Teague
  作者单位：University of California System; University of California Berkeley; Yale University; Max Planck Society; Max Planck Society; University of Konstanz; Max Planck Society; University of Konstanz; University of Louisiana System; Southeastern Louisiana University; Smithsonian Institution; Smithsonian Tropical Research Institute
  摘要：Under an accelerating biodiversity crisis, increased urbanization, habitat fragmentation, and climate change require new approaches to assess conservation impact. We argue that animal biologging is a cost-effective method for monitoring biodiversity at its source, including tracked animals and the habitats they occupy. Biologging, or animal-mounted sensors to record data, can act as a reporting, measurement, and verification system, and deliver direct insights into environments of selection. U...
• Cryo-EM structure and polar assembly of the PS2 S-layer of Corynebacterium glutamicum AI翻译
  作者：Sogues, Adria; Sleutel, Mike; Petit, Julienne; Megrian, Daniela; Bayan, Nicolas; Wehenkel, Anne Marie; Remaut, Han
  作者单位：Flanders Institute for Biotechnology (VIB); Vrije Universiteit Brussel; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; Pasteur Network; Institut Pasteur de Montevideo; Universite Paris Saclay; Centre National de la Recherche Scientifique (CNRS); CEA
  摘要：The polar-growing Corynebactertales have a complex cell envelope architecture charac-terized by the presence of a specialized outer membrane composed of mycolic acids. In some Corynebacteriales, this mytomembrane is further supported by proteinaceous surface layer or S-layer, whost function, structure, and mode of assembly remain largely enigmatic. Here, we isolated ex vivo PS2 S-layers from the industrially impor-tant Corynebacterium glutamicuu and determined its atomic structure by 3D cryo-E...
• Optimal kinetics for catalytic cycles from a single path-sampling simulation AI翻译
  作者：Bolhuis, Peter G.
  作者单位：University of Amsterdam
  摘要：A catalyst's efficiency for accelerating a reaction rate is determined by its molecular structure and interactions with the substrate. While one can predict kinetics for a particular molecular model, tuning the (potentially many) model parameters to reach a desired or optimal kinetics for a catalytic cycle is usually considered computationally prohibitively expensive, especially in solvated systems. Here, we show for a simple model representing a minimal catalytic cycle that such optimization ...
• Stress- induced translocation of the endoplasmic reticulum chaperone GRP78/BiP and its impact on human disease and therapy AI翻译
  作者：Lee, Amy S.
  作者单位：University of Southern California; University of Southern California
  摘要：Since their discoveries in the 1960s as a family of proteins produced by cells in response to stress, molecular chaperones are increasingly recognized as major regulators of cellular homeostasis in health and disease. Among the heat shock protein 70 family, the 78-kDa glucose-regulated protein (GRP78), also referred to as BiP and encoded by the HSPA5 gene, contains a signal peptide targeting it into the endoplasmic reticulum (ER). Through its interaction with the transmembrane ER stress sensor...
• Mechanistic insights into the iron-sulfur cluster- dependent interaction of the autophagy receptor NCOA4 with the E3 ligase HERC2 AI翻译
  作者：Liu, Haobo; Shen, Liqiang; Gong, Xinyu; Zhou, Xindi; Huang, Yichao; Zhou, Yuqian; Guo, Zhenpeng; Guo, Hanbo; Wang, Shichao; Pan, Lifeng
  作者单位：Chinese Academy of Sciences; Shanghai Institute of Organic Chemistry, CAS; University of Chinese Academy of Sciences, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS
  摘要：NCOA4, a dedicated autophagy receptor for mediating selective autophagy of ferritin (ferritinophagy), plays a vital role in maintaining cellular iron homeostasis. The cellular abundance of NCOA4 is regulated by the E3 ligase HERC2 that can specifically tar- get NCOA4 for proteasomal degradation under iron-- replete conditions. However, the detailed molecular mechanism governing the iron-- dependent recognition of NCOA4 by HERC2 remains elusive. Here, using multidisciplinary approaches, we syst...
• Soil nitrogen drives inverse acclimation of xylem growth cessation to rising temperature in Northern Hemisphere conifers AI翻译
  作者：Zhang, Yaling; Huang, Jian- Guo; Wang, Minhuang; Wang, Wenjin; Yang, Feiyu; Deslauriers, Annie; Fonti, Patrick; Liang, Eryuan; Maekinen, Harri; Oberhuber, Walter; Rathgeber, Cyrille B. K.; Tognetti, Roberto; Treml, Vaclav; Yang, Bao; Zhai, Lihong; Antonucci, Serena; Butto, Valentina; Camarero, J. Julio; Campelo, Filipe; Cufar, Katarina; De Luis, Martin; Fajstavr, Marek; Giovannelli, Alessio; Gricar, Jozica; Gruber, Andreas; Gryc, Vladimir; Gueney, Aylin; Jyske, Tuula; Kaspar, Jakub; King, Gregory; Krause, Cornelia; Lemay, Audrey; Lombard, Fabio; Castillo, Edurne Martinez del; Morin, Hubert; Nabais, Cristina; Noejd, Pekka; Peters, Richard L.; Prislan, Peter; Saracinoa, Antonio; V. Shishovbb, Vladimir; Vavrciks, Hanus; Vieirac, Joana; Zengd, Qiao; Rossi, Sergio
  作者单位：Chinese Academy of Sciences; South China Botanical Garden, CAS; Zhejiang University; Guangdong Academy of Sciences; Institute of Eco-environmental & Soil Sciences, Guangdong Academy of Sciences; University of Quebec; University of Quebec Chicoutimi; Swiss Federal Institutes of Technology Domain; Swiss Federal Institute for Forest, Snow & Landscape Research; Chinese Academy of Sciences; Institute of Tibetan Plateau Research, CAS; Natural Resources Institute Finland (Luke); University of Innsbruck; Universite de Lorraine; AgroParisTech; Free University of Bozen-Bolzano; Charles University Prague; Nanjing University; University of Molise; University of Quebec; University Quebec Abitibi-Temiscamingue; Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Instituto Pirenaico de Ecologia (IPE); Universidade de Coimbra; University of Ljubljana; Johannes Gutenberg University of Mainz; Mendel University in Brno; Slovenian Forestry Institute; Izmir Katip Celebi University; University of Alberta; Universita Mediterranea di Reggio Calabria; Technical University of Munich; University of Naples Federico II; Siberian Federal University; Guangdong Academy of Sciences; Guangzhou Institute of Geography, Guangdong Academy of Sciences
  摘要：Controlled experiments suggest that the seasonal build-up of nitrogen (N) limitation constrains the responses of forest autumn phenology to elevated temperatures. Therefore, rising soil N is expected to increase the delaying effects of elevated temperature on the end of the season, i.e., leaf senescence. However, the interactive effects of temperature, soil N, and aridity on xylem autumn phenology remain unknown. We conducted a wide spatial analysis from 75 conifer sites in the Northern Hemisp...