49期

最新文章

• Global hinge sites of proteins as target sites for drug binding AI翻译
  作者：Zhang, Haotian; Gur, Mert; Bahar, Ivet
  作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; State University of New York (SUNY) System; Stony Brook University; State University of New York (SUNY) System; Stony Brook University
  摘要：Hinge sites of proteins play a key role in mediating conformational mechanics. Among them, those involved in the most collective modes of motion, also called global hinges, are of particular interest, as they support cooperative rearrangements that are often functional. Yet, the utility of targeting global hinges for modulating function remains to be established. We present here a systematic study of a series of proteins resolved in drug- bound forms to examine the probabilistic occurrence of ...
• Diversification of pectoral control through motor pool extension AI翻译
  作者：Gutjahr, Ruth; Bothe, Maximilian S.; Jonsson, Thorin; Chagnaud, Boris P.
  作者单位：University of Graz
  摘要：Flexible control of pectoral appendages enables motor behaviors of vastly different strength, speed, and amplitude, as in a human playing the piano or throwing a ball. Such control necessitates a fine- tuned, coordinated activation of motoneurons, which is facilitated by spatially ordered motoneuron pools in mammals. While differently sized neurons are known to contribute to different strengths of pectoral movements, it remains unclear how these pectoral motor pools are organized in less compl...
• Wet-dry cycles cause nucleic acid monomers to polymerize into long chains AI翻译
  作者：Song, Xiaowei; Simonis, Povilas; Deamer, David; Zare, Richard N.
  作者单位：Stanford University; University of California System; University of California Santa Cruz; Vilnius University; Center for Physical Sciences & Technology - Lithuania
  摘要：The key first step in the oligomerization of monomers is to find an initiator, which is usually done by thermolysis or photolysis. We present a markedly different approach that initiates acid- catalyzed polymerization at the surface of water films or water droplets, which is the reactive phase during a wet-dry cycle in freshwater hot springs associated with subaerial volcanic landmasses. We apply this method to the oligomerization of different nucleic acids, a topic relevant to how it might be...
• Proteome- wide bioinformatic annotation and functional validation of the monotopic phosphoglycosyl transferase superfamily AI翻译
  作者：Durand, Theo; Dodge, Greg J.; Siuda, Roxanne P.; Higinbotham, Hugh R.; Arbour, Christine A.; Ghosh, Soumi; Allen, Karen N.; Imperiali, Barbara
  作者单位：Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); Imperial College London; Biogen; Boston University; Boston University
  摘要：Phosphoglycosyl transferases (PGTs) are membrane proteins that initiate glycoconjugate biosynthesis by transferring a phospho-sugar moiety from a soluble nucleoside diphosphate sugar to a membrane- embedded polyprenol phosphate acceptor. The centrality of PGTs in complex glycan assembly and the current lack of functional information make these enzymes high- value targets for biochemical investigation. In particular, the small monotopic PGT family is exclusively bacterial and represents the min...
• A widespread family of ribosomal peptide metallophores involved in bacterial adaptation to metal stress AI翻译
  作者：Leprevosta, Laura; Jungerb, Sophie; Lippensc, Guy; Guillaumeb, Celine; Sicolid, Giuseppe; Oliveirae, Lydie; Oliveirae, Enrico; Falconef, Enrico; de Santis, Emiliano; Millot, Alex Rivera-; Billon, Gabriel; Stellato, Francesco; Henry, Celine; Antoine, Rudy; Zirah, Severine; Dubiley, Svetlana; Li, Yanyan; Jacob- Dubuisson, Francoise
  作者单位：Pasteur Network; Universite de Lille; Institut Pasteur Lille; Institut National de la Sante et de la Recherche Medicale (Inserm); Centre National de la Recherche Scientifique (CNRS); CHU Lille; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Ecology & Environment (INEE); Museum National d'Histoire Naturelle (MNHN); Universite Federale Toulouse Midi-Pyrenees (ComUE); Universite de Toulouse; Institut National des Sciences Appliquees de Toulouse; INRAE; Centre National de la Recherche Scientifique (CNRS); Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); Universite de Lille; AgroParisTech; Universite Paris Saclay; INRAE; Universite Federale Toulouse Midi-Pyrenees (ComUE); Universite de Toulouse; Institut National Polytechnique de Toulouse; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); University of Rome Tor Vergata; Istituto Nazionale di Fisica Nucleare (INFN); University of Quebec; Institut national de la recherche scientifique (INRS)
  摘要：Ribosomally synthesized and posttranslationally modified peptides (RiPPs) are a structurally diverse group of natural products that bacteria employ in their survival strategies. Herein, we characterized the structure, the biosynthetic pathway, and the mode of action of a RiPP family called bufferins. With thousands of homologous biosynthetic gene clusters throughout the bacterial phylogenetic tree, bufferins form by far the largest family of RiPPs modified by multinuclear nonheme iron- depende...
• Chronologically inappropriate morphogenesis (Chinmo) is required for maintenance of larval stages of fall armyworm AI翻译
  作者：Chen, Xien; Koo, Jinmo; Arya, Surjeet Kumar; Palli, Subba Reddy
  作者单位：University of Kentucky; Northwest A&F University - China; University of California System; University of California Riverside
  摘要：Broad complex (Br- C) and eip93F (E93) transcription factors promote insect metamorphosis from larva to pupa and from pupa to adult, respectively. Recently, chronologically inappropriate morphogenesis (Chinmo) has been proposed as a larval specifier in Drosophila melanogaster. However, whether Chinmo is required for larval maintenance in lepidopteran insects, the underlying mechanisms involved in maintaining the larval stage, and its interactions with the JH signaling pathway are not well unde...
• Correlating enzymatic reactivity for different substrates using transferable data- driven collective variables AI翻译
  作者：Das, Sudip; Raucci, Umberto; Neves, Rui P. P.; Ramos, Maria J.; Parrinello, Michele
  作者单位：Istituto Italiano di Tecnologia - IIT; Universidade do Porto
  摘要：Machine learning (ML) is transforming the investigation of complex biological processes. In enzymatic catalysis, one significant challenge is identifying the reactive conformations (RC) of the enzyme:substrate complex where the substrate assumes a precise arrangement in the active site necessary to initiate a reaction. Traditional methods are hindered by the complexity of the multidimensional free energy landscape involved in the transition from nonreactive to reactive conformations. Here, we ...
• Dynamic tuning of neural stability for cognitive control AI翻译
  作者：Xu, Muyuan; Hosokawa, Takayuki; Tsutsui, Ken- Ichiro; Aihara, Kazuyuki
  作者单位：University of Tokyo; Tohoku University
  摘要：The brain is thought to execute cognitive control by actively maintaining and flexibly updating patterns of neural activity that represent goals and rules. However, while actively maintaining patterns of activity requires robustness against noise and distractors, updating the activity requires sensitivity to task-- relevant inputs. How these conflicting demands can be reconciled in a single neural system remains unclear. Here, we study the prefrontal cortex of monkeys maintaining a covert rule...
• Structural basis for the synergetic neutralization of hepatitis E virus by antibody-antibody interaction AI翻译
  作者：Zheng, Minghua; Zhou, Lizhi; Huang, Yang; Zhang, Xiao; Yu, Zihao; Yang, Chengyu; Chen, Yuanzhi; Ying, Dong; Wang, Hong; Chen, Zhenqin; Liu, Chang; Tang, Zimin; Wang, Siling; Wang, Kaihang; Yang, Kaixiang; Lin, Yanqing; Li, Tingting; Zheng, Qingbing; Zheng, Zizheng; Zhang, Jun; Yu, Hai; Li, Shaowei; Gu, Ying; Xia, Ningshao
  作者单位：Xiamen University; Xiamen University; Chongqing Medical University
  摘要：Neutralizing antibodies (nAbs) play a crucial role in virology, antibody drug development, and vaccine research. In this study, we investigated the synergistic effect of two hepatitis E virus (HEV) nAbs, 8H3, and 8C11, which have exhibited enhanced neutralizing activity in a rhesus monkey model. We presented crystal structures of 8H3 Fab alone and a triple complex of 8C11 Fab and 8H3 Fab simultaneously binding to the HEV E2s protein (8C11:E2s:8H3). Through structural analysis, we identified cr...
• Identification of a depupylation regulator for an essential enzyme in Mycobacterium tuberculosis AI翻译
  作者：Kahne, Shoshanna C.; Yoo, Jin Hee; Chen, James; Nakedi, Kehilwe; Iyer, Lakshminarayan M.; Putzel, Gregory; Samhadaneh, Nora M.; Pironti, Alejandro; Aravind, L.; Ekiert, Damian C.; Bhabha, Gira; Rhee, Kyu Y.; Darwin, K. Heran
  作者单位：New York University; Cornell University; Weill Cornell Medicine; National Institutes of Health (NIH) - USA; NIH National Library of Medicine (NLM); Johns Hopkins University
  摘要：In Mycobacterium tuberculosis (Mtb), proteins that are posttranslationally modified with a prokaryotic ubiquitin-like protein (Pup) can be degraded by bacterial proteasomes. A single Pup-ligase and depupylase shape the pupylome, but the mechanisms regulating their substrate specificity are incompletely understood. Here, we identified a depupylation regulator, a protein called CoaX, through its copurification with the depupylase Dop. CoaX is a pseudopantothenate kinase that showed evidence of b...