A widespread family of ribosomal peptide metallophores involved in bacterial adaptation to metal stress

• 成果类型: Article
• 署名作者: Leprevosta, Laura; Jungerb, Sophie; Lippensc, Guy; Guillaumeb, Celine; Sicolid, Giuseppe; Oliveirae, Lydie; Oliveirae, Enrico; Falconef, Enrico; de Santis, Emiliano; Millot, Alex Rivera-; Billon, Gabriel; Stellato, Francesco; Henry, Celine; Antoine, Rudy; Zirah, Severine; Dubiley, Svetlana; Li, Yanyan; Jacob- Dubuisson, Francoise
• 署名单位: Pasteur Network; Universite de Lille; Institut Pasteur Lille; Institut National de la Sante et de la Recherche Medicale (Inserm); Centre National de la Recherche Scientifique (CNRS); CHU Lille; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Ecology & Environment (INEE); Museum National d'Histoire Naturelle (MNHN); Universite Federale Toulouse Midi-Pyrenees (ComUE); Universite de Toulouse; Institut National des Sciences Appliquees de Toulouse; INRAE; Centre National de la Recherche Scientifique (CNRS); Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); Universite de Lille; AgroParisTech; Universite Paris Saclay; INRAE; Universite Federale Toulouse Midi-Pyrenees (ComUE); Universite de Toulouse; Institut National Polytechnique de Toulouse; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Chemistry (INC); University of Rome Tor Vergata; Istituto Nazionale di Fisica Nucleare (INFN); University of Quebec; Institut national de la recherche scientifique (INRS)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10407
• DOI: 10.1073/pnas.2408304121
• 发表日期: 2024-12-03
• 关键词: oxidative stress; natural-products; nmr experiments; copper; biosynthesis; resistance; proteins; n-15; c-13

摘要

Ribosomally synthesized and posttranslationally modified peptides (RiPPs) are a structurally diverse group of natural products that bacteria employ in their survival strategies. Herein, we characterized the structure, the biosynthetic pathway, and the mode of action of a RiPP family called bufferins. With thousands of homologous biosynthetic gene clusters throughout the bacterial phylogenetic tree, bufferins form by far the largest family of RiPPs modified by multinuclear nonheme iron- dependent modification is installed in conjunction with a partner protein of the DUF2063 family. reveal that bufferins are involved in copper homeostasis, and their metal- binding under copper stress by complexing excess metal ions. Our study thus describes a homeostasis mechanism in bacteria.