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作者：Francia, Valentina; Zhang, Yao; Cheng, Miffy Hok Yan; Schiffelers, Raymond M.; Witzigmann, Dominik; Cullis, Pieter R.
作者单位：University of British Columbia; Utrecht University; Utrecht University Medical Center
摘要：Lipid nanoparticle (LNP) formulations are a proven method for the delivery of nucleic acids for gene therapy as exemplified by the worldwide rollout of LNP-based RNAi therapeutics and mRNA vaccines. However, targeting specific tissues or cells is still a major challenge. After LNP administration, LNPs interact with biological fluids (i.e., blood), components of which adsorb onto the LNP surface forming a layer of biomolecules termed the biomolecular corona (BMC) which affects LNP stability, bi...
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作者：Lin, Pei- Yi; Chen, Lulu Y.; Zhou, Peng; Lee, Sung-Jin; Trotter, Justin H.; Sudhof, Thomas C.
作者单位：Stanford University; University of California System; University of California Irvine; Stanford University; Howard Hughes Medical Institute
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作者：Tintori, Sophia C.; Caglar, Derin; Ortiz, Patrick; Chyzhevskyi, Ihor; Mousseau, Timothy A.; Rockman, Matthew, V
作者单位：New York University; New York University; University of South Carolina System; University of South Carolina Columbia
摘要：The 1986 disaster at the Chornobyl Nuclear Power Plant transformed the surrounding region into the most radioactive landscape known on the planet. Whether or not this sudden environmental shift selected for species, or even individuals within a species, that are naturally more resistant to mutagen exposure remains an open question. In this study, we collected, cultured, and cryopreserved 298 wild nematode isolates from areas varying in radioactivity within the Chornobyl Exclusion Zone. We sequ...
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作者：Case, Marshall; Smith, Matthew; Vinh, Jordan; Thurber, Greg; Baker, David
作者单位：University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
摘要：Proteins are a diverse class of biomolecules responsible for wide- ranging cellular functions, from catalyzing reactions to recognizing pathogens. The ability to evolve proteins rapidly and inexpensively toward improved properties is a common objective for protein engineers. Powerful high- throughput methods like fluorescent activated cell sorting and next- generation sequencing have dramatically improved directed evolution experiments. However, it is unclear how to best leverage these data to...
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作者：Chatterjee, Sushmita; Kon, Edo; Sharma, Preeti; Peer, Dan
作者单位：Tel Aviv University; Tel Aviv University; Tel Aviv University; Tel Aviv University
摘要：Lipid nanoparticles (LNPs) have recently emerged as a powerful and versatile clinically approved platform for nucleic acid delivery, specifically for mRNA vaccines. A major bottleneck in the field is the release of mRNALNPs from the endosomal pathways into the cytosol of cells where they can execute their encoded functions. The data regarding the mechanism of these endosomal escape processes are limited and contradicting. Despite extensive research, there is no consensus regarding the compartm...
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作者：Eygeris, Yulia; Gupta, Mohit; Kim, Jeonghwan; Jozic, Antony; Gautam, Milan; Renner, Jonas; Nelson, Dylan; Bloom, Elissa; Tuttle, Adam; Stoddard, Jonathan; Reynaga, Rene; Neuringer, Martha; Lauer, Andreas K.; Ryals, Renee C.; Sahay, Gaurav
作者单位：Oregon State University; Yeungnam University; Oregon Health & Science University; Oregon National Primate Research Center; Oregon Health & Science University; Oregon Health & Science University
摘要：Lipid nanoparticles (LNPs) largely rely on ionizable lipids to yield successful nucleic acid delivery via electrostatic disruption of the endosomal membrane. Here, we report (Thio- lipids). The Thio- lipids can be readily synthesized via the Gewald reaction, allowing for modular lipid design with functional constituents at various positions of the thiophene ring. Through the rational design of ionizable lipid structure, we prepared 47 Thio- lipids and identified some structural criteria requir...
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作者：Guzikowski, Natalie J.; Kavalali, Ege T.
作者单位：Vanderbilt University; Vanderbilt University
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作者：Win, Poe Ei Phyu; Yang, Jiahui; Ning, Shuwang; Huang, Xiang; Fu, Gengtao; Sun, Qiming; Xia, Xing - Hua; Wang, Jiong
作者单位：Soochow University - China; Nanjing Normal University; Southern University of Science & Technology; Soochow University - China; Nanjing University
摘要：Developing cost- effective and high- performance electrocatalysts for oxygen reduction reaction (ORR) is critical for clean energy generation. Here, we propose an approach to the synthesis of iron phthalocyanine nanotubes (FePc NTs) as a highly active and selective electrocatalyst for ORR. The performance is significantly superior to FePc in randomly aggregated and molecularly dispersed states, as well as the commercial Pt/C catalyst. When FePc NTs are anchored on graphene, the resulting archi...
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作者：Xu, Na; Jiang, Xiaoyan; Zhang, Wenting; Shi, Yejie; Leak, Rehana K.; Keep, Richard F.; Ye, Qing; Yang, Tuo; Li, Sicheng; Hu, Xiaoming; Stetler, R. Anne; Bennett, Michael V. L.; Chen, Jun
作者单位：Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; US Department of Veterans Affairs; Veterans Health Administration (VHA); VA Pittsburgh Healthcare System; Geriatric Research Education & Clinical Center; Duquesne University; University of Michigan System; University of Michigan; Yeshiva University; Montefiore Medical Center; Albert Einstein College of Medicine
摘要：The endothelial lining of cerebral microvessels is damaged relatively early after cerebral ischemia/reperfusion (I/R) injury and mediates blood-brain barrier (BBB) disruption, neurovascular injury, and long - term neurological deficits. I/R induces BBB leakage within 1 h due to subtle structural alterations in endothelial cells (ECs), including reorganization of the actin cytoskeleton and subcellular redistribution of junctional proteins. Herein, we show that the protein peroxiredoxin-4 (Prx4)...
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作者：Yu, Yingying; Su, Yang; Wang, Guoxiao; Lan, Miaomiao; Liu, Jin; Martin, Ruben Garcia; Brandao, Bruna Brasil; Lino, Marsel; Li, Lei; Liu, Chang; Kahn, C. Ronald; Meng, Qingyong
作者单位：China Agricultural University; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Joslin Diabetes Center, Inc.; Army Medical University
摘要：Muscle regeneration is a complex process relying on precise teamwork between multiple cell types, including muscle stem cells (MuSCs) and fibroadipogenic progenitors (FAPs). FAPs are also the main source of intramuscular adipose tissue (IMAT). Muscles without FAPs exhibit decreased IMAT infiltration but also deficient muscle regeneration, indicating the importance of FAPs in the repair process. Here, we demonstrate the presence of bidirectional crosstalk between FAPs and MuSCs via their secret...
