18期

最新文章

• Structural rearrangements in the nucleus localize latent HIV proviruses to a perinucleolar compartment supportive of reactivation AI翻译
  作者：Kizito, Fredrick; Nguyen, Kien; Mbonye, Uri; Shukla, Meenakshi; Luttge, Benjamin; Checkley, Mary Ann; Agaponova, Anna; Leskov, Konstantin; Karn, Jonathan
  作者单位：University System of Ohio; Case Western Reserve University
  摘要：Using an immunofluorescence assay based on CRISPR - dCas9 - gRNA complexes that selectively bind to the HIV LTR (HIV Cas - FISH), we traced changes in HIV DNA localization in primary effector T cells from early infection until the cells become quiescent as they transition to memory cells. Unintegrated HIV DNA colocalized with CPSF6 and HIV capsid (CA, p24) was found in the cytoplasm and nuclear periphery at days 1 and 3 post infection. From days 3 to 7, most HIV DNA was distributed primarily i...
• Single- chain fluorescent integrators for mapping G- protein- coupled receptor agonists AI翻译
  作者：Kroning, Kayla; Gannot, Noam; Li, Xingyu; Putansu, Aubrey; Zhou, Guanwei; Sescil, Jennifer; Shen, Jiaqi; Wilson, Avery; Fiel, Hailey; Li, Peng; Wang, Wenjing
  作者单位：University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan; University of Michigan System; University of Michigan
  摘要：G protein - coupled receptors (GPCRs) transduce the effects of many neuromodulators including dopamine, serotonin, epinephrine, acetylcholine, and opioids. The localization of synthetic or endogenous GPCR agonists impacts their action on specific neuronal pathways. In this paper, we show a series of single - protein chain integrator sensors that are highly modular and could potentially be used to determine GPCR agonist localization across the brain. We previously engineered integrator sensors ...
• SHP2 regulates GluA2 tyrosine phosphorylation required for AMPA receptor endocytosis and mGluR- LTD AI翻译
  作者：Lee, Sanghyeon; Kim, Jungho; Ryu, Hyun-Hee; Jang, Hanbyu; Lee, Doeun; Lee, Seungha; Song, Jae-man; Lee, Yong-Seok; Suh, Young Ho
  作者单位：Seoul National University (SNU); Seoul National University (SNU); Seoul National University (SNU); Seoul National University (SNU)
  摘要：Posttranslational modifications regulate the properties and abundance of synaptic alpha- amino - 3 - hydroxy - 5 - methyl - 4 - isoxazolepropionic acid (AMPA) receptors that mediate fast excitatory synaptic transmission and synaptic plasticity in the central nervous system. During long - term depression (LTD), protein tyrosine phosphatases (PTPs) dephosphorylate tyrosine residues in the C - terminal tail of AMPA receptor GluA2 subunit, which is essential for GluA2 endocytosis and group I metab...
• Structural and functional reorganization of inhibitory synapses by activity- dependent cleavage of neuroligin-2 AI翻译
  作者：Xu, Na; Cao, Ran; Chen, Si- Yu; Wang, Bin; Luo, Hong- Mei; Gao, Feng; Tang, Ai- Hui
  作者单位：Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Zhengzhou University
  摘要：Recent evidence has demonstrated that the transsynaptic nanoscale organization of synaptic proteins plays a crucial role in regulating synaptic strength in excitatory synapses. However, the molecular mechanism underlying this transsynaptic nanostructure in inhibitory synapses still remains unclear and its impact on synapse function in physiological or pathological contexts has not been demonstrated. In this study, we utilized an engineered proteolysis technique to investigate the effects of ac...
• Exacerbated atherosclerosis in progeria is prevented by progerin elimination in vascular smooth muscle cells but not endothelial cells AI翻译
  作者：Benedicto, Ignacio; Carmona, Rosa M.; Barettino, Ana; Espinos-Estevez, Carla; Gonzalo, Pilar; Nevado, Rosa M.; de la Fuente-Perez, Miguel; Andres-Manzano, Maria J.; Gonzalez-Gomez, Cristina; Rolas, Loic; Dorado, Beatriz; Nourshargh, Sussan; Hamczyk, Magda R.; Andres, Vicente
  作者单位：Consejo Superior de Investigaciones Cientificas (CSIC); CSIC - Centro de Investigaciones Biologicas (CIB); Centro Nacional de Investigaciones Cardiovasculares (CNIC); CIBER - Centro de Investigacion Biomedica en Red; CIBERCV; University of London; Queen Mary University London; University of Oviedo; Instituto Universitario de Oncologia de Asturias
  摘要：Hutchinson-Gilford progeria syndrome (HGPS) is a rare disease caused by the expression of progerin, a mutant protein that accelerates aging and precipitates death. Given that atherosclerosis complications are the main cause of death in progeria, here, we investigated whether progerin - induced atherosclerosis is prevented in HGPSrev - Cdh5 - CreERT2 and HGPSrev - SM22 alpha- Cre mice with progerin suppression in endothelial cells (ECs) and vascular smooth muscle cells (VSMCs), respectively. HG...
• c- di- AMP determines the hierarchical organization of bacterial RCK proteins AI翻译
  作者：Rocha, Rita; Jorge, Joao M. P.; Teixeira-Duarte, Celso M.; Figueiredo-Costa, Ines R.; Cereija, Tatiana B.; Ferreira-Teixeira, Paula F.; Herzberg, Christina; Stuelke, Joerg; Morais-Cabral, Joao H.
  作者单位：Universidade do Porto; i3S - Instituto de Investigacao e Inovacao em Saude, Universidade do Porto; Universidade do Porto; University of Gottingen
  摘要：In bacteria, intracellular K + is involved in the regulation of membrane potential, cytosolic pH, and cell turgor as well as in spore germination, environmental adaptation, cell - to - cell communication in biofilms, antibiotic sensitivity, and infectivity. The second messenger cyclic - di - AMP (c - di - AMP) has a central role in modulating the intracellular K + concentration in many bacterial species, controlling transcription and function of K + channels and transporters. However, our unde...
• The political fallout of air pollution AI翻译
  作者：Bellani, Luna; Ceolotto, Stefano; Elsner, Benjamin; Pestel, Nico
  作者单位：Ulm University; IZA Institute Labor Economics; Centro Euro-Mediterraneo sui Cambiamenti Climatici (CMCC); Universita Ca Foscari Venezia; University College Dublin; University of London; University College London; Maastricht University; Leibniz Association; Ifo Institut
  摘要：This paper studies the effect of air pollution on voting outcomes. We use data from 60 federal and state elections in Germany from 2000 to 2018 and exploit plausibly exogenous fluctuations in ambient air pollution within counties across election dates. Higher air pollution on election day shifts votes away from incumbent parties and toward opposition parties. An increase in the concentration of particulate matter (PM10) by 10 mu g/m 3 -around two within -county SDs-reduces the vote share of in...
• New voices for a better society AI翻译
  作者：Diem, Stephanie J.; Bayon, Baindu L.; Mahmoud, Hussam; Garcia-Cazarin, Mary L.; Martin, Michael J.; Rittschof, Clare C.; Silveyra, Patricia; Boland -Reeves, Alison; Najib, Dalal; Wasson, Flannery
  作者单位：National Academies of Sciences, Engineering & Medicine; University of Wisconsin System; University of Wisconsin Madison; Saint Mary's College of California; Colorado State University System; Colorado State University Fort Collins; University of Kentucky; Indiana University System; Indiana University Bloomington; National Academies of Sciences, Engineering & Medicine
• Cryo- EM reveals a nearly complete PCNA loading process and unique features of the human alternative clamp loader CTF18-RFC AI翻译
  作者：He, Qing; Wang, Feng; O'Donnell, Michael E.; Li, Huilin
  作者单位：Van Andel Institute; Rockefeller University; Howard Hughes Medical Institute; Rockefeller University
  摘要：The DNA sliding clamp PCNA is a multipurpose platform for DNA polymerases and many other proteins involved in DNA metabolism. The topologically closed PCNA ring needs to be cracked open and loaded onto DNA by a clamp loader, e.g., the well - studied pentameric ATPase complex RFC (RFC1 - 5). The CTF18RFC complex is an alternative clamp loader found recently to bind the leading strand DNA polymerase epsilon and load PCNA onto leading strand DNA, but its structure and the loading mechanism have b...
• Experimental evidence for defect tolerance in Pb- halide perovskites AI翻译
  作者：Jasti, Naga Prathibha; Levine, Igal; Feldman, Vishay (Isai); Hodes, Gary; Aharon, Sigalit; Cahen, David
  作者单位：Bar Ilan University; Bar Ilan University; Weizmann Institute of Science; Helmholtz Association; Helmholtz-Zentrum fuer Materialien und Energie GmbH (HZB); Weizmann Institute of Science; Hebrew University of Jerusalem
  摘要：The term defect tolerance (DT) is used often to rationalize the exceptional optoelectronic properties of halide perovskites (HaPs) and their devices. Even though DT lacked direct experimental evidence, it became a fact in the field. DT in semiconductors implies that structural defects do not translate to electrical and optical effects (e.g., due to charge trapping), associated with such defects. We present pioneering direct experimental evidence for DT in Pb- HaPs by comparing the structural q...